Abstract: A cryogenic device is disclosed which has a cryogenic chamber, temperature sensor, a liquid cryogen injection zone, a source of heat, and a controller, in response to temperature data obtained from the temperature sensor, the injection of liquid cryogen into the injection zone and the application of heat, to control the temperature in the cryogenic chamber. The device may utilise a second source or a higher temperature gas as the source of heat and may utilise a multilayered plate insulator between the injection sites for cryogen and the cryogenic chamber. The device may also incorporate an overflow monitoring system and/or an automated cryogen filling system.