Abstract: An operation method of a multiplex slide plate device is provided. First, the multiplex slide plate device is assembled, including a slide plate, a sacrificial layer and a housing. The slide plate has reaction vessels, and the sacrificial layer has a microfluidic channel composed of an injection channel, a main channel and a distal channel. A sample solution is injected to the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into the reaction vessels. Afterwards, an oil is injected to the injection channel, such that the oil flows from the injection channel through the main channel to the distal channel, wherein the oil removes the sample solution not loaded into the reaction vessels. Next, the sacrificial layer is heated to melt, and the melted sacrificial layer is mixed with the oil.

Abstract: An early detection and prediction method of pan-cancer is provided, including the following steps. A microRNA expression profile database of cancer patient populations and healthy populations is established, and an early detection and prediction method of pan-cancer is developed based on the microRNA expression profile database. Next, a microRNA expression profile of a liquid biopsy sample of a subject is analyzed via the early detection and prediction method of pan-cancer, so as to determine whether the subject may already have cancer.

Abstract: The disclosure provides a health risk assessment method including the following steps. A miRNA expression database of a healthy population is established. A miRNA expression in a plasma specimen of a subject is analyzed. After that, miRNA expression data of the subject is compared with the miRNA expression in the miRNA expression database of the healthy population, and the miRNA expression being too high or too low in the plasma specimen of the subject is found out to assess health risk of the subject.

Abstract: A multiplex slide plate device is provided, including a slide plate, a sacrificial layer, and a housing. The slide plate has reaction vessels arranged in an array. The sacrificial layer has a microfluidic channel, which has an injection channel, a main channel, and a distal channel connected to each other. The housing is used to accommodate the slide plate and the sacrificial layer, and is composed of a cover and a tray, and the cover has an injection hole, an exhaust hole, and a storage tank. A sample solution and an oil are injected from the injection hole into the injection channel, wherein the sample solution is pushed by the oil. The sample solution loads into each of the reaction vessels while flowing through the main channel. Excess waste liquid flows from the exhaust hole into the storage tank, and is covered by the oil and cannot reflow.

Abstract: An operation method of a multiplex slide plate device is provided. First, the multiplex slide plate device is assembled, including a slide plate, a sacrificial layer and a housing. The slide plate has reaction vessels, and the sacrificial layer has a microfluidic channel composed of an injection channel, a main channel and a distal channel. A sample solution is injected to the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into the reaction vessels. Afterwards, an oil is injected to the injection channel, such that the oil flows from the injection channel through the main channel to the distal channel, wherein the oil removes the sample solution not loaded into the reaction vessels. Next, the sacrificial layer is heated to melt, and the melted sacrificial layer is mixed with the oil.

Abstract: A multiplex slide plate device and an operation method thereof are provided. The multiplex slide plate device includes a slide plate and a sacrificial layer. The slide plate has reaction vessels arranged in an array, an injection hole and an exhaust hole, wherein each of the reaction vessels has an opening portion and a bottom portion. The sacrificial layer has a microfluidic channel, wherein the microfluidic channel has an injection channel, a main channel and a distal channel connected to each other. The sacrificial layer is assembled to the slide plate, wherein the main channel faces the opening portion. A sample solution is injected into the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into each of the reaction vessels while flowing through the main channel.

Abstract: A measuring method for amplicon length is provided. A qPCR master mix, a forward primer, a reverse primer, a hybridization probe, a double-stranded DNA binding dye, and nucleic acid samples are added into reaction wells for qPCR reaction, and the fluorescence intensity of each of a hybridization probe and a double-stranded DNA binding dye varying with cycle number is respectively measured. Afterwards, amplicon length is obtained by applying a calculating method.

Abstract: A thermal cycler device is provided, including an annular conveying element having a circular conveying path, a plurality of slide plate device holding elements, a plurality of heating blocks, a pressing element and a cooling device. The annular conveying element operates in stages, such that the slide plate device holding elements move along the circular conveying path while carrying a plurality of slide plate devices. When each slide plate device holding element moves to the corresponding heating block, the annular conveying element stops operating and the pressing element performs the pressing process, such that each slide plate device contacts the corresponding heating block for heat transfer.

Abstract: A nucleotide sequence having SEQ ID NO:1, a universal reverse primer having SEQ ID NO:2, and a universal RT primer are provided. In the miRNA detection method, the universal RT primer is used for the cDNA synthesis of a miRNA sample and the universal reverse primer is used for cDNA molecule amplification in qPCR quantitative detection. The universal reverse primer sequence, the nucleotide sequence, and the design rules are used in the method for designing primer, so as to design a primer for qPCR quantitative detection.

Abstract: A digital and quantitative PCR (dqPCR) measuring method for a nucleic acid sample is provided, including providing a testing plate having a plurality of reaction wells, so as to perform a dqPCR reaction on the nucleic acid sample. Especially for the nucleic acid sample to be tested having a plurality of nucleic acid targets with different concentration ranges of wide variations, digital PCR and quantitative PCR can be performed simultaneously, so as to quantify the copy number of nucleic acid targets.

Abstract: A multiplex slide plate device and an operation method thereof are provided. The multiplex slide plate device includes a slide plate and a sacrificial layer. The slide plate has reaction vessels arranged in an array, wherein each of the reaction vessels has an opening portion and a bottom portion. The sacrificial layer has a microfluidic channel, wherein the microfluidic channel has an injection channel, a main channel and a distal channel connected to each other. The sacrificial layer is assembled to the slide plate, wherein the main channel faces the opening portion. A sample solution is injected into the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into each of the reaction vessels while flowing through the main channel.

Abstract: A nucleotide sequence having SEQ ID NO:1, a universal reverse primer having SEQ ID NO:2, a universal RT primer, a method for designing primer, and a miRNA detection method are provided. In the miRNA detection method, the universal RT primer is used for the cDNA synthesis of a miRNA sample and the universal reverse primer is used for cDNA molecule amplification in qPCR quantitative detection. The universal reverse primer sequence, the nucleotide sequence, and the design rules are used in the method for designing primer, so as to design a primer for qPCR quantitative detection.

Abstract: A multiplex slide plate device is provided, including a slide plate, a sacrificial layer, and a housing. The slide plate has reaction vessels arranged in an array. The sacrificial layer has a microfluidic channel, which has an injection channel, a main channel, and a distal channel connected to each other. The housing is used to accommodate the slide plate and the sacrificial layer, and is composed of a cover and a tray, and the cover has an injection hole, an exhaust hole, and a storage tank. A sample solution and an oil are injected from the injection hole into the injection channel, wherein the sample solution is pushed by the oil. The sample solution loads into each of the reaction vessels while flowing through the main channel. Excess waste liquid flows from the exhaust hole into the storage tank, and is covered by the oil and cannot reflow.

Abstract: A nucleotide sequence having SEQ ID NO:1, a universal reverse primer having SEQ ID NO:2, and a universal RT primer are provided. In the miRNA detection method, the universal RT primer is used for the cDNA synthesis of a miRNA sample and the universal reverse primer is used for cDNA molecule amplification in qPCR quantitative detection. The universal reverse primer sequence, the nucleotide sequence, and the design rules are used in the method for designing primer, so as to design a primer for qPCR quantitative detection.