Abstract: The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.
Type:
Application
Filed:
July 20, 2012
Publication date:
April 25, 2013
Applicant:
Raindance Technolgies, Inc.
Inventors:
Benjamin J. Miller, Qun Zhong, Darren Roy Link
Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
Type:
Application
Filed:
February 16, 2012
Publication date:
August 30, 2012
Applicant:
RAINDANCE TECHNOLGIES, INC.
Inventors:
Michael Samuels, Jeffrey Charles Olson, Andrew Watson, Keith Brown, Darren Roy Link