Abstract: New succinic acid derivatives of the formula ##STR1## wherein A represents hydrogen, a naphthoxyethyl group of formula (b), ##STR2## or a substituted phenoxyethyl group of the formula ##STR3## and in this latter formula R stands for hydrogen or C.sub.1-4 alkyl,R.sup.1 is hydrogen, halo or C.sub.1-4 alkyl, andR.sup.2 and R.sup.3 each stand for hydrogen or halo,and agriculturally acceptable salts thereof are prepared by reacting succinic anhydride with an aryloxyethanol of the formula ##STR4## The new succinic acid drivatives of the formula (I) possess plant growth regulating properties.

Abstract: Glucoamylase is immobilized with a carbodiimide on a polymer having amino or carboxy functional groups. When the polymer contains amino groups, the glucoamylase is treated with the carbodiimide and then mixed with the polymer or the carbodiimide is added to a mixture of the glucoamylase and the polymer. When the polymer contains carboxy groups, the polymer is treated with the carbodimide and then mixed with the glucomylase. The polymer is produced from acrylic acid, metharylic acid, acrylamide, methacrylamide, N-amino-ethyl-acrylamide and/or N-amino ethyl methacrylamide and a cross-linking agent of the acrylic or allylic type.

Abstract: The invention relates to a process for the immobilization of compounds comprising nucleophilic groups.According to the process of the invention a polymer comprising an amido group is swollen in a buffer. For this purpose any buffer is suitable which contains no amino, sulfhydryl and/or hydroxy groups in the molecule. To the swollen polymer a solution of a quinone - preferably p-benzoquinone - in a water miscible organic solvent is added and the mixture is activated at 273.degree.-343.degree. K. for 0.5-48 hours - preferably for 24 hours. The unreacted quinone is washed out with an aqueous solution of a water miscible organic solvent and thereafter water and/or a buffer. To the purified gel at a pH value between 3 and 11 - preferably between 6 and 8 - a buffered solution of a compound comprising a nucleophilic group is added, the suspension is incubated at 260.degree.-313.degree. K. for 24 hours, the gel is separated and the non-bound compound comprising a nucleophilic group is removed by washing.

Abstract: The invention relates to a new polyacrylamide adhesive suitable for the fixing of fine-particle sorbent layers based on organic and/or inorganic compounds, used in overpressured one- and multilayer chromatographic procedures.The said adhesive is prepared by polymerizing the aqueous solutions of acrylamide and N,N'-methylene-bis-acrylamide in the presence of a catalyst pair at from 21.degree. to 30.degree. C., pulpifying the precipitate thus obtained and repeating the precipitation and the pulpification more times.

Abstract: Carboxypeptidase B enzyme is isolated by a process wherein homogenized and autolyzed pancreas is subjected to a thermal treatment, and carboxypeptidase B is separated by fractionation with ammonium sulfate. This process produces carboxypeptidase B having satisfactory specific activity while eliminating the use of pancreas cell fluid or an acetonous powder from pancreas as a starting material. The isolated carboxypeptidase B may be immobilized on a polymer gel containing carboxy groups activated with a carbodiimide.

Abstract: The invention relates to a new dyestuff composition for histological examinations, which comprises(1) a product formed by the interaction of one molar proportion of one or more thionine dyestuffsselected from thionine (Colour Index 52,000), toluidine blue (Colour Index 52,040) and dimethyl thionine (Azur A) with at least 0.2 molar proportion of resorcine at elevated temperature,(2) 0.5 to 2 moles of a dyestuff "A", calculated for one mole of the thionine dyestuff interacted with resorcine, and(3) 0.5 to 2 moles of a dyestuff "B", calculated for one mole of the thionine dyestuff interacted with resorcine, whereindyestuff "A" and dyestuff "B" are alkaline dyestuffs different in colour and pH, and both dyestuff "A" and dyestuff "B" can be mixtures of alkaline dyestuffs similar in colour and pH.

Abstract: The invention relates to a process for the preparation of a cyclodextrine glycosyltransferase enzyme.The immobilization can be carried out by two methods. According to the first method a cyclodextrine glycosyltransferase enzyme is activated with a solution of a carbodiimide compound and the thus treated enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polysaccharide derivative comprising at least 0.1 m-equiv./g of free amino group. According to the second method the enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polymer produced from an acrylic acid and/or methacrylic acid or acrylic amide and/or methacrylic amide monomer by means of a cross-linking agent of the acrylic or allylic type which polymer comprises at least 0.1 m-equiv./g of functional carboxy group, activating the polymer with a solution of a carbodiimide compound and washing and if desired drying the product thus obtained.

Abstract: Immobilized cholinesterase enzyme preparations are prepared by treating a polymeric resin, built up from acrylic acid and/or methacrylic acid and acryl amide and/or methacryl amide monomers with an acryl or allyl type cross-linking agent and containing at least 0.1 meq/g of --COOH functional groups, with a carbodiimide derivative which is soluble in water or is soluble in an organic solvent at temperatures below 0.degree. C., applying a solution of cholinesterase enzyme with a pH of 4.5 to 8.5 to the resulting activated support, washing the resulting product, and drying it if desired.

Abstract: The invention relates to new gonadoliberin derivatives of the formula (I)Glp-His-Trp-Ser-Tyr-X-Leu-Arg-Pro-Y, (I)whereinY represents a glycine-amide group or an -NH-alkyl group having 1 to 4 carbon atoms in the alkyl moiety,X stands for a D-thyroxyl, D-thyronyl or D-4-chlorophenylalanyl group,and acid addition salts and metal complexes thereof.The new nona- and decapeptide derivatives have an excellent luteinizing and folliculus stimulating hormone releasing activity, and they can therefore be used as active ingredients in pharmaceutical compositions for human or veterinary application.

Abstract: Aminoacylase is isolated from mammal kidneys by comminuting and homogenizing mammal kidney in water, centrifuging to form an aqueous extract, heating the aqueous extract at 60.degree. to 80.degree. C. for 5 to 15 minutes, centrifuging, adding a salt such as ammonium sulfate to the resultant supernatant, centrifuging to separate solids, dissolving the solids in water, dialyzing and recovering active aminoacylase from the dialyzed solution. The process produces aminoacylase with high specific activity and the process requires fewer purification steps. The aminoacylase obtained may be utilized directly without any subsequent purification to produce immonobilized aminoacylase. Immobilization can be carried out by covalent bonding of the aminoacylase to a partially hydrolyzed Akrilex P type acryl amide-N,N'-methylene-bis(arylamide) copolymer. The recovered aminoacylase can be subjected to two chromatographic purification steps to produce a very pure aminoacylase.

Abstract: The invention relates to a new method for the preparation of N-aryl-N'-(mono- or disubstituted)-urea derivatives having the general formula (I), ##STR1## wherein Aryl is an optionally substituted phenyl group, andR.sup.1 and R.sup.2 each stand for an optionally substituted alkyl, cycloalkyl, alkoxy or phenyl group, orR.sup.1 and R.sup.2 may form, together with the adjacent nitrogen atom, a nitrogen-containing heterocyclic group which may contain a further hetero atom, orone of R.sup.1 and R.sup.2 may also stand for hydrogen,with the proviso that if one of R.sup.1 and R.sup.2 is an optionally substituted phenyl group, the other may represent only hydrogen atom or an optionally substituted alkyl or alkoxy group,by reacting a carbamate of the general formula (II) with an amine of the general formula (III),R.sup.1 R.sup.2 N--CO--X (II)Aryl--NH.sub.2 (III)or a carbamate of the general formula (IV) with an amine of the general formula (V),Aryl--NH--CO--X (IV)R.sup.1 R.sup.2 NH (V)wherein R.sup.1, R.sup.

Abstract: The invention relates to a new process for the selective hydroxymethylation of substituted ortho- or paranitrotoluenes of the general formula (I) ##STR1## in order to prepare nitrophenylalkanols of the general formula (II) ##STR2## In the above formulae the nitro group is attached to the benzene ring in ortho or para position related to the methyl group or the hydroxyalkyl side chain, respectively, X stands for hydrogen, fluorine or an alkyl, alkoxy or nitro group, and n is 1, 2 or 3.According to the process of the invention the starting substance is hydroxymethylated with an excess of formaldehyde at a temperature above room temperature in dimethyl formamide and in the presence of a strong base. The reaction is performed in dimethyl formamide containing 0.1 to 1% by weight of water, and the reaction is directed to the formation of the required end-product by properly adjusting the temperature and the amount of formaldehyde and the base with respect to the starting compound of the general formula (I).