Abstract: The present specification discloses a method capable of automatically recognizing an accurate wound boundary and a method of generating a 3D wound model based on the recognized wound boundary. The method of automatically recognizing a wound boundary according to the present specification is a method of automatically recognizing a wound boundary based on artificial intelligence, and may photograph several frames of the wound to be recognized with an RGB-D camera, separating measurement information in an image, amplifying image data for learning, and passing the amplified image data through an artificial neural network. The method may include generating a three-dimensional (3D) model by performing boundary recognition post-processing on the data passing through the artificial neural network to match a two-dimensional (2D) image with the 3D model.

Abstract: A method of controlling a three-dimensional (3D) bioprinter includes a nozzle end aligning operation (S100) that includes an operation (a) in which a nozzle end alignment sensor is installed at a predetermined position on a bed in a printing chamber and a sensing point of the nozzle end alignment sensor is positioned at an origin position of the bed, an operation (b) in which the bed is moved toward one side in an X-axis direction and the sensing point of the nozzle end alignment sensor is positioned under a nozzle of a first print module, an operation (c) in which the first print module is moved downward and a Z value of a nozzle end of the first print module is measured, an operation (d) in which the first print module is positioned at an original position and the bed is moved toward the other side in the X-axis direction to position the sensing point of the nozzle end alignment sensor under a syringe, which is disposed at a printing position, of a second print module, an operation (e) in which the second p

Abstract: A three-dimensional (3D) bioprinter includes: a case, a printing chamber surrounded by wall surfaces, a moving unit including a horizontal moving unit installed in a space under a bottom surface of the printing chamber and a vertical moving unit installed outside a side surface of the printing chamber, a bed disposed above a bottom surface opening of the bottom surface of the printing chamber, a first bellows which covers a space between the bed and an inner circumferential surface of the bottom surface opening, a first print module provided in the printing chamber and installed to be vertically movable by the vertical moving unit in a Z-axis direction, a second print module provided at one side of the first print module in the printing chamber and installed to be vertically movable by the vertical moving unit in the Z-axis direction, and a controller.

Abstract: The present invention method for manufacturing a tissue regeneration patch includes: A) preparing a micronized adipose tissue extract; B) injecting the micronized adipose tissue extract into a mold of a predetermined shape; C) cooling the mold into which the adipose tissue extract is injected at a temperature between ?25° C. and ?10° C.; and D) removing the mold to obtain a tissue regeneration patch.

Abstract: The present disclosure relates to a bio printer to which a biomaterial freeze-hardening method is applied and a freeze-hardening method thereof.

Abstract: The present invention relates to a 3D bioprinter. The 3D bioprinter, according to the present invention, comprises: a case inside of which a work space is provided; a printing plate installed inside of the case so as slide in the forward, backward, left, and right directions; a first nozzle installed inside the case for dispensing a biomaterial in a solid state on the printing plate; a second nozzle installed inside the case for dispensing a biomaterial in a liquid state on the printing plate; and a control unit for controlling the dispensing by the first nozzle and the second nozzle, wherein the first nozzle and the second nozzle are used to print a single structure by stacking the biomaterial in the solid state and the biomaterial in the liquid state.

Abstract: The present disclosure relates to a bio printer to which a biomaterial freeze-hardening method is applied and a freeze-hardening method thereof.

Abstract: The present invention relates to a chamber environment controlling apparatus for a three-dimensional bioprinter including a printing chamber having an internal space in which printing is performed and which is defined by wall surfaces including sidewalls. The apparatus may include a temperature adjustor including a heater configured to heat the internal space of the printing chamber and an air circulator including an air guide portion extending from an outside of the printing chamber along a sidewall on one side and formed so that air flowing in through an inlet moves along an inside, is heated by the temperature adjustor, and is discharged into the internal space of the printing chamber through an outlet, a circulation fan configured to circulate air along the air guide portion, and a filter configured to filter the air circulating along the air guide portion.

Abstract: The present invention provides a composition for kidney treatment using the omentum, a medical kit for kidney treatment including the composition for kidney treatment using the omentum, and a film for kidney treatment including a cured product of the composition for kidney treatment using the omentum.

Abstract: The disclosure is a three-dimensional bioprinter including a multi-syringe printing module. The multi-syringe printing module includes a packing plated installed inside a hollow cylinder portion and configured to separate an inside of the cylinder portion into a upper zone and a lower zone, a rotary shaft for rotating the packing plate, a plurality of syringe holders disposed below the packing plate and configured to hold each syringe, a syringe holder support portion configured to support the syringe holder; and a rotary shaft driving unit for rotating the rotary shaft. The syringe holder holding the syringe selected for printing is rotated to an output position by the rotation of the rotary shaft by the rotary shaft driving unit. A lift up/lift down unit having a vertical movement unit disposed outside the printing chamber is provided to control lift up/lift down of the syringe printing module.

Abstract: The present invention relates to a method for using a lyophilization hyaline cartilage powder to produce a composition for regenerating cartilage, and a composition for regenerating cartilage produced by using the method, the method comprising: A) a step for preparing hyaline cartilage; B) a step for freeze-drying and crushing the hyaline cartilage, and producing a lyophilization hyaline cartilage powder; C) a step for producing an adipose tissue extract from autologous adipose tissue; and D) a step for producing a composition which is for regenerating cartilage and including the lyophilization hyaline cartilage powder and the adipose tissue extract.

Abstract: A method of controlling a three-dimensional (3D) bioprinter includes a nozzle end aligning operation (S100) that includes an operation (a) in which a nozzle end alignment sensor is installed at a predetermined position on a bed in a printing chamber and a sensing point of the nozzle end alignment sensor is positioned at an origin position of the bed, an operation (b) in which the bed is moved toward one side in an X-axis direction and the sensing point of the nozzle end alignment sensor is positioned under a nozzle of a first print module, an operation (c) in which the first print module is moved downward and a Z value of a nozzle end of the first print module is measured, an operation (d) in which the first print module is positioned at an original position and the bed is moved toward the other side in the X-axis direction to position the sensing point of the nozzle end alignment sensor under a syringe, which is disposed at a printing position, of a second print module, an operation (e) in which the second p

Abstract: A three-dimensional (3D) bioprinter according to the present invention includes a case, a printing chamber surrounded by wall surfaces so that an interior thereof is isolatable from the outside thereof, a moving unit including a horizontal moving unit installed in a space under a bottom surface of the printing chamber and a vertical moving unit installed outside a side surface of the printing chamber, a bed which is disposed above a bottom surface opening of the bottom surface of the printing chamber, a first bellows which covers a space between the bed and an inner circumferential surface of the bottom surface opening to isolate the interior of the printing chamber from the space under the bottom surface of the printing chamber, a first print module provided in the printing chamber and installed to be vertically movable by the vertical moving unit in a Z-axis direction, a second print module provided at one side of the first print module in the printing chamber and installed to be vertically movable by the v

Abstract: The present specification relates to a method for manufacturing a multilayered cell sheet, and a multilayered cell sheet manufactured using same, the method comprising the steps of: (a) forming a first cell layer on a first substrate, which has a melting point or is changed from being hydrophobic to being hydrophilic at any one temperature from 0° C. to 30° C.; (b) forming a second cell layer on a second substrate to be degraded by an enzyme; (c) making the first cell layer and the second cell layer come in contact with each other; (d) selectively removing the first substrate by providing a temperature lower than or equal to the melting point of the first substrate or a temperature at which the first substrate is changed to being hydrophilic; and (e) selectively removing the second substrate by making the second substrate come in contact with a solution containing the enzyme.

Abstract: The specification relates to a method of manufacturing a diabetic foot patient-specific skin regeneration sheet, and a diabetic foot patient-specific skin regeneration sheet.

Abstract: The present specification relates to a bioink composition for cartilage regeneration, a method for manufacturing a customized scaffold for cartilage regeneration using same, and a customized scaffold for cartilage regeneration using said manufacturing method, the bioink composition comprising: a first liquid comprising an adipose tissue-derived stromal vascular fraction, hyaline cartilage powder, and fibrinogen; and a second liquid comprising thrombin.

Abstract: The present invention relates to a bioink composition for a dermis regeneration sheet and a method for manufacturing a customized dermis regeneration sheet using same, the bioink composition comprising: a first liquid comprising an adipose tissue-derived stromal vascular fraction, an extracellular matrix, and fibrinogen; and a second liquid comprising thrombin.

Abstract: Disclosed are a three-dimensional printer of a fused deposition modeling and software setting values thereof, which blocks transmitting calories of a nozzle upwards and simultaneously heats a lower aluminum output plate at a high temperature to locally transfer heat in a sandwich form to where an output proceeds, such that it is possible to realize miniaturization of engineering plastic printing at low energy, prevent the shrinkage of a printed object, and maximize adhesive strength between layers, wherein the three-dimensional printer is further provided with a nozzle made of SUS having excellent heat resistance to use an engineering plastic filament having a high melting temperature and obtain optimal quality within a numerical range of predefined software setting values, wherein the output plate is provided with a hard anodized aluminum plate so that both commodity plastic and engineering plastic material can be used without a need to attach an additional special sheet.

Abstract: Disclosed are a three-dimensional printer of a fused deposition modeling and software setting values thereof, which blocks transmitting calories of a nozzle upwards and simultaneously heats a lower aluminum output plate at a high temperature to locally transfer heat in a sandwich form to where an output proceeds, such that it is possible to realize miniaturization of engineering plastic printing at low energy, prevent the shrinkage of a printed object, and maximize adhesive strength between layers, wherein the three-dimensional printer is further provided with a nozzle made of SUS having excellent heat resistance to use an engineering plastic filament having a high melting temperature and obtain optimal quality within a numerical range of predefined software setting values, wherein the output plate is provided with a hard anodized aluminum plate so that both commodity plastic and engineering plastic material can be used without a need to attach an additional special sheet.

Abstract: The present invention relates to a 3D bioprinter. The 3D bioprinter, according to the present invention, comprises: a case inside of which a work space is provided; a printing plate installed inside of the case so as slide in the forward, backward, left, and right directions; a first nozzle installed inside the case for dispensing a biomaterial in a solid state on the printing plate; a second nozzle installed inside the case for dispensing a biomaterial in a liquid state on the printing plate; and a control unit for controlling the dispensing by the first nozzle and the second nozzle, wherein the first nozzle and the second nozzle are used to print a single structure by stacking the biomaterial in the solid state and the biomaterial in the liquid state.