Abstract: The invention provides methods for determining the identity of an unknown live microorganism in a mixed culture. The microorganism can be a bacterium, fungus, virus, or protozoan. The invention further provides an assay for determining the ability of a selected microorganism in a mixed culture to replicate in the presence of a chemical agent. Kits for determining the identity of a live microorganism in a mixed culture and for determining the ability of a microorganism in a mixed culture to replicate are also provided.

Abstract: This invention provides for a novel amplification procedure for nucleic acid. The method uses a mutant RNA polymerase designed to transcribe deoxynucleotides. Using primers that bind to target nucleic acid the primers form polymerase recognition sites that permit transcription of the target in an isothermal and logrithmic manner, with the added advantage of forming multiple single stranded copies, which are readily detected by hybridization assays.

Abstract: This invention provides for a novel amplification procedure for nucleic acid. The method uses a wild type or mutant RNA polymerase designed to transcribe both deoxyribonucleotides and ribonucleotides.

Abstract: This invention provides for a novel amplification procedure for nucleic acid. The method uses a mutant RNA polymerase designed to transcribe deoxynucleotides. Using primers that bind to target nucleic acid the primers form polymerase recognition sites that permit transcription of the target in an isothermal and logrithmic manner, with the added advantage of forming multiple single stranded copies, which are readily detected by hybridization assays.

Abstract: The invention provides a method for determining the identity of an unknown live microorganism in a mixed culture. The microorganism can be a bacterium, fungus, virus, or protozoan. The invention further provides an assay for determining the ability of a selected microorganism in a mixed culture to replicate in the presence of a chemical agent. Kits for determining the identity of a live microorganism in a mixed culture and for determining the ability of a microorganism in a mixed culture to replicate are also provided.

Abstract: Methods for detecting in a human biological sample the presence of HLA DQB1 alleles 0201/0202, 0302/0303, and/or 0602 which are associated with increased or decreased susceptibility to insulin dependent diabetes mellitus.

Abstract: A method of determining the presence of a nucleic acid analyte from a sample comprising binding a biotinylated capture oligonucleotide to a solid phase non-covalently coated with streptavidin or an avidin derivative. The capture oligonucleotide is contacted with and selectively hybridized to, under stringent hybridization conditions, a nucleic acid analyte to form a detectable hybridization complex.

Abstract: A centrifuge sample tube phase separation plug is disclosed for use in conjunction with sample tube to effect organic solvent separation on a volumetric basis. The centrifuge sample tube is of the type having a conical bottom end closed at the apex with an upward extending cylindrical portion opening to the top of the sample tube. A centrifuge sample tube cap is provided to close the sample tube with the mixed organic solvent and sample. The sample tube cap contains a plug having a central aperture and a tapered profile for fitting into the top of the conical bottom section of the centrifuge sample tube. Presuming that the sample tube is loaded and capped with mixed organic solvent and sample, centrifugation occurs. Into the centrifugation process, the plug releases from the cap and passes from the cap area, through the sample, to the top of the conical portion of the centrifuge sample tube.