Abstract: A method of depositing single particles onto a target includes loading a particle suspension to a droplet dispenser having a suspension reservoir and a nozzle section, detecting particles in the nozzle section, testing a single particle condition of the droplet dispenser, and determining whether an ejection region of the nozzle section includes one single particle. The method further includes operating the droplet dispenser for dispensing a droplet such that the droplet is dispensed onto the target if the single particle condition is fulfilled, or the droplet is dispensed into a collection reservoir if the single particle condition is not fulfilled. The step of testing the single particle condition further includes determining whether a sedimentation region adjacent to the ejection region is free of particles. A dispenser for performing the method is also provided.

Abstract: A method of electron microscopy imaging of samples, using an electron microscope (100) having a microscope column (10) and a transfer device (11) with a grid carriage (12), comprises the steps of preparing multiple samples (1) on a single electron microscopy grid (2), including dispensing the samples (1) with a dispenser device (30) on distinct positions on the grid (2), introducing the grid (1) with the transfer device (11) into the microscope column (10), and electron microscopy imaging of the samples (1), wherein the preparing step includes holding the grid (2) on the grid carriage (12) of the transfer device (11) or on a grid holder device (20) provided at the electron microscope (100) and dispensing the samples (1) on the grid (2) while holding it on the grid carriage (12) or on the grid holder device (20). Furthermore, an electron microscope (100) for electron microscopy imaging of samples is described.

Abstract: A method of depositing single particles onto a target comprises the steps of loading a particle suspension to a droplet dispenser having a suspension reservoir and a nozzle section, detecting particles in the nozzle section, testing a single particle condition of the droplet dispenser, wherein it is determined whether an ejection region of the nozzle section includes one single particle, and operating the droplet dispenser for dispensing a droplet, wherein the droplet is dispensed onto the target, if the single particle condition is fulfilled, or the droplet is dispensed into a collection reservoir, if the single particle condition is not fulfilled, wherein the step of testing the single particle condition further includes determining whether a sedimentation region adjacent to the ejection region is free of particles. Furthermore, a dispenser apparatus dispensing single particles onto a target is described.

Abstract: A method of depositing at least one substance on a target substrate (1) comprises the step of operating at least one droplet dispenser (21) such that droplets (2) including the at least one substance are deposited on the target substrate (1), wherein the target substrate (1) has a substrate surface including spatially delimited receptacle sections (3) being arranged for accommodating the droplets (2), and the at least one droplet dispenser (21) is controlled in dependency on the locations of the receptacle sections (3) such that the droplets (2) are directed onto the receptacle sections (3). Furthermore, a dispenser device (100) for depositing at least one substance on a target substrate (1) is described.

Abstract: A method for depositing samples, in which at least one sample (10) is arranged on a substrate (30), comprises the following steps: positioning a sample dispenser (20) above the substrate (30), and actuating the sample dispenser (20) so that the sample (10) is moved from the sample dispenser (20) along a trajectory (11) to a predefined deposition position (32) on the substrate (30), wherein at least part of the trajectory (11) is shielded against electrical interference fields. Also described is a substrate (30) for receiving samples (10), comprising a substrate body (31), on the surface of which at least one deposition position (32) is provided, and a shielding electrode (40), which is designed so as to electrostatically shield the space above the at least one deposition position (32) against electrical interference fields.

Abstract: A process for the production of a reaction chamber assembly, wherein a flat substrate and bottomless reaction chambers are provided, the substrate is first loaded with a biological agent and then the bottomless reaction chambers are bonded glue-free to the substrate, in particular through laser bonding, and liquid-tight reaction chambers, for instance individual wells, individually connected wells, such as strips, or wells in the form of a microtiter plate, are obtained. The present invention further provides a kit comprising a substrate suitable for being loaded with at least one biological agent and at least one bottomless reaction chamber, wherein the kit is suitable for glue-free bonding of the bottomless reaction chamber to the substrate.

Abstract: The invention relates to a microdispenser (1) for dispensing a liquid sample in a dispensing device, with a sample container (2) for receiving the liquid sample, and with a nozzle (7) for dispensing the sample located in the sample container (2). The microdispenser (1) with the filled sample container (2) can in this case be stored independently of and fluidically separately from the dispensing device, without the sample escaping from the sample container (2) during storage.

Abstract: The invention relates to a microdispenser (1) for dispensing a liquid sample in a dispensing device, with a sample container (2) for receiving the liquid sample, and with a nozzle (7) for dispensing the sample located in the sample container (2). The microdispenser (1) with the filled sample container (2) can in this case be stored independently of and fluidically separately from the dispensing device, without the sample escaping from the sample container (2) during storage.

Abstract: The invention relates to a microdispenser (1) for dispensing a liquid sample in a dispensing device, with a sample container (2) for receiving the liquid sample, and with a nozzle (7) for dispensing the sample located in the sample container (2). The microdispenser (1) with the filled sample container (2) can in this case be stored independently of and fluidically separately from the dispensing device, without the sample escaping from the sample container (2) during storage.