Abstract: Disclosed is a device and method for preparing composite ice glaze, relating to the field of freshness of aquatic products. The device includes a frame portion, a mother liquid preparation portion, an ice glaze preparation portion, an ice glaze post-processing portion and a controller system; the frame portion includes a shell, a base, and a thermal insulation baffles; the mother liquid preparation portion includes a main water inlet pipe, a water storage tank, mother liquid tanks; the ice glaze preparation portion includes a homogenizing tank, a homogenizing tank inlet pipe, a waste cylinder inlet pipe; the ice glaze post-processing portion includes a reception cylinder, and a waste cylinder. The device provided by the invention is simple to use, and the prepared concentration is accurate and effective, so that the compounding process can be simplified and the compounding efficiency is effectively improved.

Abstract: The present invention discloses a recirculating aquaculture system (RAS) and method for shrimp culture through SBR wastewater treatment. The RAS includes a control cabinet, culture tanks, a clean water tank, a clean water filling pipeline, a drainage pipeline, a buried activated sludge tank and a solid-liquid separation mechanism. The solid-liquid separation mechanism comprises a sludge pump, an input pipe, a plate and frame type filter press and a liquid output pipe. An input end of the sludge pump is immersed in sewage of the buried activated sludge tank; and a clean water output end of the plate and frame type filter press is connected with the buried activated sludge tank through the liquid output pipe.

Abstract: A radio frequency heating method includes: cladding an object to be heated by using a surrounding medium, and performing radio frequency heating operation on the object to be heated clad by the surrounding medium, in which the surrounding medium is an alcoholic solution. In the radio frequency heating method according to the embodiments of the present application, by using the alcoholic solution as the surrounding medium to clad the object to be heated, heating uniformity of the object to be heated in a radio frequency heating process may be improved, edge corner energy concentration effect in the radio frequency heating process may be fully avoided, and then quality of the object to be heated after heating may be ensured.

Abstract: A hydrolysate compound protein for larval largemouth bass (Micropterus salmoides) and use thereof for aquatic animal feeds. The micro-diet was formulated with the following components: 72-74 parts by weight of a hydrolyzed protein pre-mixture, 3-5 parts by weight of mixed fish soluble meal, 4-6 parts by weight of gluten flour, 2-4 parts by weight of sodium caseinate, and 0.4-0.6 parts by weight of a yeast hydrolysate, where the hydrolyzed protein pre-mixture includes white fish meal, fermented soybean meal, shrimp meal and blood meal with the weight ratio of 58-62: 5-7: 4-6: 2-3. The compound protein is obtained by fully pulverizing the foregoing components, passing through a 100-mesh sieve, fully mixing in proportion, and being hydrolyzed with neutral protease and keratinase.

Abstract: Disclosed is an automatic immersion ice-coating machine, including a frame, an ice-coating working pool, a feed port, a conveying device, a control system and a driving system. A first side of the ice-coating working pool communicates with a buffer tank. A bottom of the ice-coating working pool is stepped or inclined, and a drain valve is provided at a bottom of the ice-coating working pool. The conveying device includes a first conveyor belt and a second conveyor belt. The first conveyor belt is located under the feed port, and a lower portion of the first conveyor belt is lower than the liquid level, and the second conveyor belt communicates with the discharge port. The first and second conveyor belts are made of stainless steel mesh belts, and surfaces of the stainless steel mesh belts are provided with separators in a spaced manner.

Abstract: Disclosed is a multi-view fast photographing device for horny jaws of cephalopods. The device includes a cover, a cover pin, a stylus, an image generating device, a data display device, and an image acquisition and processing device; a top of the image generating device is open and provided with mounting holes, and the cover is connected with the mounting holes through the cover pin and movably covers the top of the image generating device. The image acquisition and processing device is fixed at a bottom of the image generating device. The data display device is fastened to a front side wall of the image generating device. A magnetic patch is fixed on a front surface of a baffle of the data display device. The stylus is magnetically attracted to the magnetic patch.

Abstract: This disclosure relates to cold-chain transportation, and more particularly to a refrigerated container refrigeration system capable of preventing freezing of container door, including compressors, oil separators, gas coolers, regenerators, electronic expansion valves, gas-liquid separators, an evaporator, suction pressure regulating valves, oil-level solenoid valves, gas cooler pressure regulating valves, differential pressure regulating valves, an evaporation pressure regulating valve, solenoid valves, check valves, flow meters, pressure sensors, temperature sensors, a door anti-freezing area, a refrigerated container shell, refrigerated container doors, a refrigeration unit, an anti-freezing pipeline and fastening components. Carbon dioxide is selected as refrigerant. A flow two-stage cycle compression refrigeration system with switchable operation pipeline is adopted, and the outlet pipeline of a high-pressure compressor is extended for preventing freezing of container door.

Abstract: A method of predicting a central fishing ground of flying squid family Ommastrephidae, includes three steps of setting spatial and temporal dimension, setting environmental factor, and establishing a central fishing ground prediction model. The spatial and temporal dimension includes three levels of spatial dimensions, and two levels of temporal dimensions of week and month. An SST is selected as a main environmental factor, and two environmental factors, i.e., SSH and Chl-a, are selected as a supplement. The environmental factors include four situations. According to the setting situations of the spatial and temporal dimension and the environmental factor, a set of sample schemes of 24 situations is established using permutation and combination method. An error backward propagation neural network model is established, wherein an input layer inputs data of the sample scheme set, and an output layer outputs a CPUE or a fishing ground grading index converted from the CPUE.

Abstract: A method of preparing a ddx27-deletion zebrafish mutant, including: determining a target of ddx27 knockout on a sixth exon of the ddx27 in a zebrafish and designing a gRNA sequence; using primers T7-ddx27-sfd and tracr rev for PCR amplification with a pUC19-gRNA scaffold plasmid as a template; purifying and transcribing the PCR product obtained in vitro to produce gRNA; introducing the gRNA and a Cas9 protein into the zebrafish; and culturing the zebrafish to obtain a zebrafish ddx27 mutant of stable inheritance. In addition, the application also discloses a phenotype of the ddx27-deletion zebrafish mutant, which plays an important role in investigating the biological function.

Abstract: A mobile quantitative feeding-spraying integrated system and method. The system includes at least one hull equipped with a propeller, a feeder housing fixedly arranged on the hull, a feed box arranged on the feeder housing, a flip assembly provided at an upper end of the feed box, a blanking device arranged in the feeder housing to quantitatively control the bait blanking amount, a casting device and a spraying device. An upper end of the blanking device is a feeding end, which supports and is connected to the lower end of the feed box. A lower end of the blanking device is the discharging end. An upper side of the casting device is connected with the lower end of the blanking device, and the lower side of the casting device is provided with a discharging end facing the aquaculture water body.

Abstract: The present invention relates to refrigerated trucks, more particularly to a front-mounted external cold air intake system for a large refrigerated truck, including a refrigerated compartment, an indoor chiller unit, an outdoor chiller unit, a front-mounted air duct, air outlets, horizontal louvers, folding elbows, windshields, blade control knobs, filters, covers, wind deflectors and blades. In the front-mounted cold air intake system, the front-mounted air duct introduces outdoor cold air into the refrigerated compartment, and transmits and distributes the cold air through the air outlets. Moreover, the air window structures formed by the horizontal shutters, the folding elbows and the windshields are used for discharging air, so as to stabilize the pressure in the refrigerated compartment. The system of the present invention uses outdoor cold air to effectively cool the compartment allowing for energy saving of the refrigerated truck.

Abstract: A method of constructing a zebrafish notch1a mutant using CRISPR/Cas9 technique. The method includes: determining a target for knocking out notch1a; using primers T7-notch1a-sfd and tracr rev for PCR amplification with a pUC19-gRNA scaffold plasmid as a template; transcribing PCR product in vitro followed by purification to obtain gRNA; and microinjecting the gRNA and a Cas9 mRNA into a zebrafish embryo followed by culture to obtain an notch1a mutant of stable inheritance. The invention selects a specific target and utilizes CRISPR/Cas9 technique to knock out the notch1a in the zebrafish without destroying other genes, generating the zebrafish notch1a mutant. Moreover, the invention also discloses the phenotype of the zebrafish notch1a mutant, which plays a significant role in studying the effect of the Notch1a receptor in the Notch signaling pathway.

Abstract: A method for preparing an intelligent antibacterial and antioxidative film involves preparing a PVA solution; adding nano-TiO2 to the PVA solution to obtain a PVA-TiO2 solution; determining the optimal amount of nano-TiO2; preparing a PSPC solution; preparing a PSPC-TiO2-PVA solution; and producing a PSPC/TiO2/PVA film. The film has better mechanical performance than saccharide and protein films. Shelf life of food is prolonged as the film possesses antibacterial and antioxidative properties. Furthermore, the film shows different colors in various pH environments. The film has a wide range of applications in food packaging owing to the integration of color development and antibacterial and antioxidative properties.

Abstract: Disclosed is a pond culture circulation system based on a bio-toilet module including a culture pond and an annular treatment channel surrounding the culture pond. The annular treatment channel is sequentially provided with a sedimentation zone, a biological purification zone and a water storage zone along a direction of water flow. The culture pond includes a plurality of culture pond units. Each of the plurality of culture pond units is provided with a bio-toilet module in communication with the sedimentation zone through a sewage pipe. The water storage zone communicates with each of the plurality of culture pond units through a gravity-flow culvert. The invention further discloses a use method of the pond culture circulation system based on the bio-toilet module.

Abstract: A floating plate type marine refrigerated container system capable of generating electricity by using wave energy that relates to ocean energy, including a machine room, retractable pipes, vertical rise-fall pipes, vertical connecting rods, floating plates, controllers, a rising-falling control button, an indicator light, push-pull handles, grooves, fixed supports, generator sets, wires, a storage battery, a connecting block, a machine room door, a heat dissipation window, sliding areas, a movable plate, a displacement sensor, hinges, sliding shafts, sliding groove groups and the electrical equipment of the refrigerated container. The wave energy in the vertically moving waves is collected by the floating plate, and converted into electric energy. The electric energy are gathered to the storage battery, and finally supplied to the electrical equipment of the refrigerated container.

Abstract: A buoyage for emergency communication and a corresponding rescue method are provided. The buoyage for emergency communication includes: a positioning device, a buoyancy block, a plurality of compression springs, an optical fiber, a reel, a frame, an optical fiber slip ring, a pure iron disk, an optical fiber connector and an oil-filled electromagnet, wherein the positioning device is fixed on a top portion of the buoyancy block, and is configured to emit a position signal of itself when the buoyage returns to a surface of water; the buoyancy block is provided above the frame, wherein the compression springs are provided between the buoyancy block and the frame; the pure iron disk is fixedly on a center of a bottom portion of the buoyancy block. The oil-filled electromagnet is adsorbed to the pure iron disk after being energized to limit a vertical displacement of the buoyancy block.

Abstract: The gene editing technology was used to carry out target knockout of zc3h12b gene of Oryzias latipes, establishing Oryzias latipes missing Zc3h12b protein product. The established Oryzias latipes all show different degrees of liver lesions such as hepatobiliary duct hyperplasia and fusion, hepatocyte steatosis, and fibrosis. With increase of months, significant fatty liver appears with local cyst necrosis, obvious lymphocyte infiltration in liver sinusoids and abnormal increase in number of macrophages. Human tumor markers cytokeratin 19 (CK19), smooth muscle actin (SMA) and glypican-3 (GPC3) positive cells are also detected. It is suggested that ZC3H12B can be used as a treatment target and a biomarker for biliary cystadenoma (BCA), biliary cystadenocarcinoma (BCAC), or fatty liver or liver cancer related to BCA or BCAC. The zc3h12b missing Oryzias latipes can be used as an animal model in researches on pathological processes of BCA, BCAC, or fatty liver or liver cancer related to BCA or BCAC.

Abstract: An elliptical and funnel-shaped jet nozzle structure includes a plurality of elliptical tapered diversion channels, a plurality of elliptical jet nozzles and a conveyer belt. A wall thickness of each of the elliptical tapered diversion channels is 1-5 mm. A wall thickness of each of the elliptical jet nozzles is 1-5 mm. A thickness of the conveyer belt is 1-5 mm. The elliptical tapered diversion channel is a hollow elliptical truncated cone and it includes an upper opening and a lower opening. The upper opening of the elliptical tapered diversion channel is connected to an elliptical opening, and the lower opening of the elliptical tapered diversion channel is connected to an inlet of the elliptical jet nozzle. The elliptical jet nozzle is a hollow elliptical cylinder.

Abstract: A slender and funnel-shaped jet nozzle structure includes a plurality of slender tapered diversion channels, a plurality of slender jet nozzles and a conveyer belt. The wall thickness of the slender tapered diversion channel is 1-5 mm. The wall thickness of the slender jet nozzle is 1-5 mm. A thickness of the conveyer belt is 1-5 mm. The slender tapered diversion channel is a hollow slender truncated cone and includes an upper opening and a lower opening. The upper opening of the slender tapered diversion channel is substantively elliptic, and the lower opening of the slender tapered diversion channel is connected to an inlet of the slender jet nozzle. The slender jet nozzle is a hollow slender elliptic cylinder.

Abstract: A cephalopod fishery forecasting method in northwest African waters based on environmental factors, including the following steps: step 1: acquiring catch production statistical data from cephalopod fisheries in northwest African waters of many years; step 2: acquiring marine environmental data corresponding to the catch production statistical data, the marine environmental data including sea surface temperature (SST) and sea surface height anomaly (SSHA); step 3: studying the relationship between the operating haul, the operating output ratio and the average output per haul in each interval as indexes of a central fishery and the marine environmental data of step 2; and step 4: establishing suitability indexes (SI) of different environmental factors, and calculating habitat suitability indexes (HSI) under different weight cases by using an expert assignment method, thus obtaining distribution waters of the central fishery of cephalopod fisheries in northwest African waters for forecasting the central fishery