Patents Assigned to Shanghai Pulmonary Hospital
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Patent number: 10503150Abstract: The present invention discloses a method for precise localization and treatment of a target site, which builds a 3D digital model of an anatomical structure of a patient in accordance with tomographic image data of the patient; a structure model for locating a target site which comprises a template model for locating a target site and an angle locating auxiliary unit model is customized, and a position and an angle for loading and treating a target site are designed according to 3D position of the target site; then a target site locating structure is printed by 3D printing technology, and the target site locating structure is utilized to treat the target site.Type: GrantFiled: August 10, 2017Date of Patent: December 10, 2019Assignee: SHANGHAI PULMONARY HOSPITALInventors: Chang Chen, Mu Li, Lei Zhang, Zeyao Li, Long Wang, Donglai Chen, XierMaiMaiTi Kadeer, Yawei Gu, Ziwen Fan
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Patent number: 10278978Abstract: Present invention relates to a human lung tissues-active targeting immune nanoliposome of methylprednisolone, wherein, nanoliposomes loaded with therapy drugs is covalently coupled with nanobodies against human pulmonary surfactant protein A. Wherein, the therapy drug is methylprednisolone sodium succinate, the nanoliposome consists of phospholipids, cholesterols and long cycling materials. The molar ratio of the methylprednisolone sodium succinate to phospholipids within the nanoliposome is 0.30-0.45. Present invention successfully provides a new human lung tissues targeting hormone preparation, wherein, the nanoliposome serves as a carrier, the nanobody against human pulmonary surfactant protein A serves as a specific lung tissue targeting ligand, methylprednisolone sodium succinate serves as a therapy drug. In accordance with present invention, an efficient, stable human lung tissues-active targeting immune nanoliposome, with specific active lung targeting, is prepared.Type: GrantFiled: November 27, 2015Date of Patent: May 7, 2019Assignee: SHANGHAI PULMONARY HOSPITALInventors: Huiping Li, Zhaofang Yin, Xian He, Shanmei Wang
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Publication number: 20180267507Abstract: The present invention discloses a method for precise localization and treatment of a target site, which builds a 3D digital model of an anatomical structure of a patient in accordance with tomographic image data of the patient; a structure model for locating a target site which comprises a template model for locating a target site and an angle locating auxiliary unit model is customized, and a position and an angle for loading and treating a target site are designed according to 3D position of the target site; then a target site locating structure is printed by 3D printing technology, and the target site locating structure is utilized to treat the target site.Type: ApplicationFiled: August 10, 2017Publication date: September 20, 2018Applicant: Shanghai Pulmonary HospitalInventors: CHANG CHEN, Mu Li, Lei Zhang, Zeyao Li, Long Wang, Donglai Chen, XierMaiMaiTi Kadeer, Yawei Gu, Ziwen Fan
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Patent number: 9908934Abstract: The present invention relates to pharmaceutical and medical technologies, and more particularly to novel nanobodies against pulmonary surfactant protein A (SP-A) and their preparation methods. The nanobodies of the present invention comprises an amino acid sequence having certain formula. The present invention also relates to nucleic acid sequences encoding the nanobodies, their preparation method and their applications. Immunohistochemistry and in vivo imaging show that the nanobodies of the present inventions have high lung-targeting specificity.Type: GrantFiled: November 23, 2015Date of Patent: March 6, 2018Assignees: Shanghai Pulmonary Hospital, TONGJI UNIVERSITY SCHOOL OF MEDICINEInventors: Huiping Li, Shanmei Wang
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Patent number: 9879073Abstract: The present invention relates to the field of biochemistry and pharmaceutical technologies. The present invention provides nanobodies that bind to human pulmonary surfactant protein A (SP-A) as well as the preparing methods and use of the same. The nanobody comprise an amino acid sequence having the formula of Q(x)2LVESGG(x)2V(x)2G(x)SL(x)LS(x)24E(x)n2 KG(x)4S(x)n3T(x)2Y(x)C(x)n4S(x)n5V(x)n6R; wherein x is amino acid; n2˜n6 are positive integers; 1?n2?21; 1?n3?19; 1?n4?50; 1?n5?22; 1?n6?8. The present invention take fresh frozen sections of lung as antigen, gene sequences with high affinity with hSP-A were obtained by constructing an SP-A antibody library and affinity selection, and nanobodies with high affinity and small molecular weight were obtained by induced expression of the gene sequences through a prokaryotic expression vector. Immunohistochemistry and in vivo imaging in nude mice showed the nanobodies have high specificity for targeting lung tissue.Type: GrantFiled: April 8, 2015Date of Patent: January 30, 2018Assignee: SHANGHAI PULMONARY HOSPITALInventors: Huiping Li, Xian He, Shanmei Wang
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Publication number: 20170058021Abstract: The present invention relates to the field of biochemistry and pharmaceutical technologies. The present invention provides nanobodies that bind to human pulmonary surfactant protein A (SP-A) as well as the preparing methods and use of the same. The nanobody comprise an amino acid sequence having the formula of Q(x)2LVESGG(x)2V (x)2G(x) SL(x) LS(x)24E (x)n2KG(x)4S(x)n3T(x)2Y(x) C(x)n4S(x)n5V(x)n6R; wherein x is any amino acid; n2˜n6 are positive integers; 1?n2?21; 1?n3?19; 1?n4?50; 1?n5?22; 1?n6?8. The present invention take fresh frozen sections of lung as antigen, gene sequences with high affinity with hSP-A were obtained by constructing an SP-A antibody library and affinity selection, and nanobodies with high affinity and small molecule weight were obtained by induced expression of the gene sequences through a prokaryotic expression vector. Immunohistochemistry and in vivo imaging in nude mice showed the nanobodies have high specificity for targeting lung tissue.Type: ApplicationFiled: April 8, 2015Publication date: March 2, 2017Applicant: Shanghai Pulmonary HospitalInventors: Huiping Li, Xian He, Shanmei Wang
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Patent number: 9228010Abstract: The present invention relates to pharmaceutical and medical technologies, and more particularly to novel nanobodies against pulmonary surfactant protein A (SP-A) and their preparation methods. The nanobodies of the present invention comprises an amino acid sequence having certain formula. The present invention also relates to nucleic acid sequences encoding the nanobodies, their preparation method and their applications. Immunohistochemistry and in vivo imaging show that the nanobodies of the present inventions have high lung-targeting specificity.Type: GrantFiled: August 2, 2013Date of Patent: January 5, 2016Assignee: Shanghai Pulmonary Hospital, Tongji University School of MedicineInventors: Huiping Li, Shanmei Wang
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Publication number: 20140314832Abstract: The present invention relates to pharmaceutical and medical technologies, and more particularly to novel nanobodies against pulmonary surfactant protein A (SP-A) and their preparation methods. The nanobodies of the present invention comprises an amino acid sequence having certain formula. The present invention also relates to nucleic acid sequences encoding the nanobodies, their preparation method and their applications. Immunohistochemistry and in vivo imaging show that the nanobodies of the present inventions have high lung-targeting specificity.Type: ApplicationFiled: August 2, 2013Publication date: October 23, 2014Applicant: Shanghai Pulmonary Hospital, Tongji University School of MedicineInventors: Huiping Li, Shanmei Wang