Abstract: Aspects of the disclosure relate to compositions and methods for delivering a transgene (e.g., a transgene encoding one or more gene products) to a target cell. The disclosure is based, in part, on adeno-associated virus (AAV) capsid protein variants characterized by tropisms for certain cell types (e.g., neurons, muscle cells, bone cells, heart cells, etc.). In some embodiments, recombinant A A Vs (rAAVs) comprising the capsid protein variants (e.g., AAVv66, SEQ ID NO: 1) are more efficiently packaged than rAAVs having certain wild-type AAV capsid proteins. Methods of delivering an rAAV comprising the AAV capsid protein variants are also described by the disclosure.

Abstract: A method for diagnosing and predicting operation conditions of large-scale equipment based on feature fusion and conversion, including: collecting a vibration signal of each operating condition of the equipment, and establishing an original vibration acceleration data set of the vibration signal; performing noise reduction on the original vibration acceleration data set, and calculating a time domain parameter; performing EMD on a de-noised vibration acceleration and calculating a frequency domain parameter; constructing a training sample data set through the time domain parameter and the frequency domain parameter; establishing a GBDT model, and inputting the training sample data set into the GBDT model; extracting a leaf node number set from a trained GBDT model; performing one-hot encoding on the leaf node number set to obtain a sparse matrix; and inputting the sparse matrix into a factorization machine to obtain a prediction result.

Abstract: Disclosed is a method of synthesizing a (1R,2R)-nitroalcohol compound of formula (I), as shown in the following reaction scheme, including: subjecting a compound of formula (II) and a compound of formula (III) to a condensation reaction in an organic solvent in the presence of a copper complex generated in situ from a chiral (1S,2R)-amino alcohol ligand and a cupric salt to produce the (1R,2R)-nitroalcohol compound of formula (I), where R1 and R2 are defined in the same manner as that in the specification. The method involves mild reaction conditions, excellent diastereoselectivity and high chemical yield, and thus it is suitable for industrial applications.

Abstract: A method for fabrication of a 3D printed part with high through-plane thermal conductivity is provided, where pure polymer particles and a carbon-based filler for heat conduction are subjected to milling and mixing in the mechanochemical reactor disclosed in Chinese patent ZL 95111258.9 under the controlled milling conditions including milling pan surface temperature, milling pan pressure, and number of milling cycles; then a resulting mixture is extruded to obtain 3D printing filaments; and finally, the 3D printing filaments are used to fabricate the 3D printed part with high through-plane thermal conductivity through fused deposition modeling (FDM) 3D printing. The fabrication method can realize the fabrication of a 3D printed part with high through-plane thermal conductivity through the FDM 3D printing technology, features simple process, continuous production, etc., and is suitable for the industrial production of thermally-conductive parts with complex structures.

Abstract: Disclosed are a tumor biomarker and application thereof, and a tumor detection kit. Patients suffering from non-small cell lung cancer, containing an EML4-ALK or SLC34A2-ROS1 fusion gene, have a specific circular RNA in blood. The base sequences of the circular RNA are represented by SEQ ID NOS. 1-6. The circular RNA is associated with non-small cell lung cancer, stably and constantly exists in plasma samples, and has high specificity and effectiveness, and thus can be used as a biomarker for tumor detection. Reagents such as primers and probes for detecting the circular RNA can be used for preparing the tumor detection kit.

Abstract: A method for assessing fatigue damage and a fatigue life based on a crystal plastic welding process model. According to the new method, consideration is given to the effects of the crystal slip system and the polycrystal plastic strain on the welding process performance of the material. A welding process damage and fatigue life assessment model is established on the mesoscopic scale. The effect of microscopic characterizations of materials on the welding process performance, as well as on the fatigue damage and life of welded joints, can be studied from the mesoscopic point of view. The relationship between the welding process and the evolution of the material performance can be determined by the macro-mesoscopic coupling calculation model to further determine the effect and degree of welding processes on the fatigue damage and life of materials.

Abstract: The disclosure in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the disclosure relates to gene transfer methods using the recombinant adeno-associated viruses. In some aspects, the disclosure relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.

Abstract: A liquid storage and controlled-release device and a biological detection chip. The liquid storage and controlled-release device comprises a liquid storage capsule with a liquid storage body which is deformable under a pressure, and a sealing layer for sealing the liquid storage body. A support platform is provided right below the liquid storage capsule and tightly connected with the liquid storage capsule, wherein, a directional release chamber is provided at the middle of the support platform, and the directional release chamber is provided with a guiding chamber for collecting the liquid and a sharp edge for inducing break. Compared with the conventional technology, the opening for liquid release is at the end far away from the rotation center, so that the liquid can be released completely by the centrifugal force, ensuring the quantitative release of stored liquid and reducing the influence on the accuracy of the subsequent detection.

Abstract: A valve system for driving fluid and a method for using the same are provided. The valve system includes a fluid unit far away from the rotation center, a fluid unit close to the rotation center, a fluid transferring unit and a gas path pipeline for communicating the fluid unit close to the rotation center with the fluid unit far away from the rotation center. A rotation radius of a fluid outlet of the fluid unit far away from the rotation center is greater than that of a fluid inlet of the fluid unit close to the rotation center. The fluid outlet of the fluid unit far away from the rotation center is located at an end thereof away from the rotation center, and the fluid inlet of the fluid unit close to the rotation center is located at an end thereof close to the rotation center.

Abstract: A vacuum interrupter. The vacuum interrupter comprising at least one anode side contact blade, at least one cathode side contact blade, at least one anode side conducting rod, at least one a cathode side conducting rod, a shielding case and an outer magnet, wherein the shielding case covers the at least one anode side contact blade, the c at least one anode side contact blade, the at least one anode side conducting rod and at least one cathode side conducting rod; and the outer magnet covers the shielding case. According to the vacuum interrupter, the outer magnet is arranged to generate a fixed first longitudinal magnetic field in the interelectrode area of the at least one anode side contact blade and the at least one cathode side contact blade, contraction of arcs during current breaking can be alleviated, the arcs are in a diffusion state, and ablation of the contact in the arcing process is reduced, and breaking capacity of a vacuum circuit breaker is guaranteed.

Abstract: A method for fabrication of a 3D printed part with high through-plane thermal conductivity is provided, where pure polymer particles and a carbon-based filler for heat conduction are subjected to milling and mixing in the mechanochemical reactor disclosed in Chinese patent ZL 95111258.9 under the controlled milling conditions including milling pan surface temperature, milling pan pressure, and number of milling cycles; then a resulting mixture is extruded to obtain 3D printing filaments; and finally, the 3D printing filaments are used to fabricate the 3D printed part with high through-plane thermal conductivity through fused deposition modeling (FDM) 3D printing. The fabrication method can realize the fabrication of a 3D printed part with high through-plane thermal conductivity through the FDM 3D printing technology, features simple process, continuous production, etc., and is suitable for the industrial production of thermally-conductive parts with complex structures.

Abstract: A method for electrolysis-ozone-corrosion inhibitor/electrolysis-ozone-hydrogen peroxide-corrosion inhibitor coupling treatment on toxic and refractory wastewater includes the following steps: adding toxic and refractory wastewater to be treated into a wastewater treatment reaction tank equipped with a plate anode and a plate cathode, and starting a direct current (DC) power supply connected to the plate anode and the plate cathode to treat the toxic and refractory wastewater at an appropriate current density under stirring, during which a corrosion inhibitor and hydrogen peroxide are added to the toxic and refractory wastewater to be treated and ozone is introduced into the toxic and refractory wastewater to be treated through an aeration device. The method can increase the production rate and production quantity of free radicals in a reaction system, effectively improve the treatment efficiency for toxic and refractory wastewater, and reduce the treatment cost.

Abstract: The disclosure provides a superjunction IGBT (insulated gate bipolar transistor) device, wherein a carrier storage layer of a first conductivity type is provided between a voltage sustaining layer and a base region, and a MISFET (metal-insulator-semiconductor field effect transistor) of a second conductivity type is also integrated in a cell, with at least one gate of the MISFET is connected to the emitter contact thereof. The MISFET is turned off at a low forward conduction voltage, helping to reduce the conduction voltage drop. The MISFET can provide a path for carriers of a second conductivity type and prevent the carrier storage layer from suffering a high electric field when the forward conduction voltage is slightly higher or it is at the forward blocking state, helping to improve the reliability.

Abstract: A tanning agent-free leather making method is provided. A raw hide is subjected to controllable dehydration with a molecular sieve-polar organic solvent composite dehydration media to achieve a dehydrated hide, and then a tanning agent-free leather is prepared by increasing the surface roughness of the dehydrated hide and reducing the surface energy of the dehydrated hide. The present disclosure does not use any metal or non-metal tanning agent, which is eco-friendly. The present disclosure breaks the balance of water distribution between the raw hide and the polar organic solvent, which realizes a multi-medium self-driven directional controllable dehydration of the raw hide, effectively solving the problems during dehydration of the raw hide only using polar organic solvent. The molecular sieve-polar organic solvent dehydration system is easy to recover and can be recycled for the controllable dehydration of the raw hide multiple times. Therefore, a brand-new clean leather making technology is provided.

Abstract: A functional ink suitable for 3D printing and a preparation method thereof are provided. The ink includes the following components in parts by weight: 0.5-1.5 parts of a regulator, 1-5 parts of a conductive material, 0.1-0.5 parts of a crosslinking agent, 0.1-0.5 parts of a catalyst, and 10-80 parts of a solvent. The prepared functional ink has a self-healing function at room temperature, eliminating the interface resistance between printing layers and improving the mechanical strength between the layers. Moreover, the prepared functional ink has excellent electrical conductivity and a variety of electrical, magnetic, and electrochemical properties, and can be applied in the fields of functional materials and devices such as energy storage, electromagnetic shielding and stress sensing.

Abstract: The present invention provides use of a Pseudomonas aeruginosa vaccine in the manufacture of a medicament for the prevention and treatment of respiratory system disease. The Pseudomonas aeruginosa vaccine of the present invention can effectively prevent and treat pulmonary infection caused by multidrug-resistant Pseudomonas aeruginosa and COPD complicated with Pseudomonas aeruginosa infection by activating the specific immune response of the body. The Pseudomonas aeruginosa vaccine of the present invention can reduce the bacterial load in the immunized subject through the established immunization procedures, thereby providing a technical solution that can effectively prevent pulmonary infection with Pseudomonas aeruginosa, which avoids the technical problems caused by the use of antibiotics such as poor effectiveness, difficulty in curing and proneness to drug resistance in the prior art to a certain degree.

Abstract: The present invention belongs to the field of microbiology, and particularly relates to membrane vesicles (MVs) isolated from bacteria, and an isolation and preparation system and method for the membrane vesicles, and applications of the membrane vesicles. The bacteria of the present invention comprise Gram-positive bacteria and Gram-negative bacteria. The invention uses ionizing irradiation to irradiate bacteria, and isolates and purifies the produced membrane vesicles. The membrane vesicles prepared can be used as a vaccine, a vaccine adjuvant and/or a pharmaceutical carrier. In addition, the present invention provides a biological composition comprising the membrane vesicles and inactivated bacteria. In addition, the present invention also provides a preparation system, and isolation and purification system for bacterial membrane vesicles and the corresponding method. The membrane vesicles obtained by using the system and method have high yield, high purity and easy to be industrialized.

Abstract: As disclosed herein, a desktop 3D display system is provided. A 2D image display module is used for receiving and displaying an integral imaging source. A viewing angle guide module is used for guiding light emitted from the integral imaging source. A light modulation module is arranged for modulating the light guided by the viewing angle guide module and reconstructing a 3D image. A rotation module is configured to enable synchronous rotation of the 2D image display module, the view angle guide module and the light modulation module, wherein. A rotation angle speed of the synchronous rotation is associated with the switching speed of the integral imaging source of the 2D image display module. For a 3D image reconstructed in a single visual area range, crosstalk created by the diffuse reflective feature of pixels on a 3D image in other visual areas can be eliminated, thereby improving the viewing experience.

Abstract: The present invention belongs to the field of microbiology, and particularly relates to an application of a Pseudomonas aeruginosa vaccine in prevention and treatment of burn and scald complicated with bacterial infection. The burn and scald of the present invention include burns and scalds, and degree of the scalds includes I degree, superficial II degree, deep II degree, or III degree scalds. Site of the scalds includes skin, mucosa or other tissues. The Pseudomonas aeruginosa vaccine of the present invention can effectively prevent and treat burn and scald complicated with Pseudomonas aeruginosa infection caused by multidrug-resistant Pseudomonas aeruginosa by activating the specific immune response of the body.

Abstract: A method for electrolysis-ozone-corrosion inhibitor/electrolysis-ozone-hydrogen peroxide-corrosion inhibitor coupling treatment on toxic and refractory wastewater includes the following steps: adding toxic and refractory wastewater to be treated into a wastewater treatment reaction tank equipped with a plate anode and a plate cathode, and starting a direct current (DC) power supply connected to the plate anode and the plate cathode to treat the toxic and refractory wastewater at an appropriate current density under stirring, during which a corrosion inhibitor and hydrogen peroxide are added to the toxic and refractory wastewater to be treated and ozone is introduced into the toxic and refractory wastewater to be treated through an aeration device. The method can increase the production rate and production quantity of free radicals in a reaction system, effectively improve the treatment efficiency for toxic and refractory wastewater, and reduce the treatment cost.