Abstract: A calibration system characterizes luminescence measurement systems, in particular spectrally resolving, wide-field and/or confocal imaging systems. The calibration system has a baseplate with at least one flow-through channel, wherein the at least one channel is formed as a sample chamber for the luminescence measurement system, at least one reservoir in communication with the at least one channel and adapted to receive a liquid, and at least one focusing device integrated into a baseplate for setting a defined measurement beam focus of the luminescence measurement system to be calibrated by using a focusing surface.
Type:
Grant
Filed:
March 17, 2006
Date of Patent:
January 29, 2013
Assignees:
Bam Bundesanstalf Fuer Materialforschung und - Pruefung, Sigma-Aldrich GmbH
Inventors:
Ute Resch-Genger, Katrin Hoffmann, Dietmar Pfeifer, Roland Nitschke, Pierre Nording
Abstract: The invention is directed to a method and a kit for calibrating a photoluminescence measurement system, in particular a fluorescence measurement system. The kit includes a number of fluorescence standards i and their corrected and certified fluorescence spectra Ii(?), whereby the fluorescence standards i are selected, so that their spectrally corrected fluorescence spectra Ii(?) cover a broad spectral range with high intensity. The standards are characterized by large half-widths FWHMi of their bands of at least 1400 cm?1. According to the method of the invention, partial correction functions Fi(?) are generated by forming the quotient of the measured fluorescence spectra Ji(?) and the corresponding corrected fluorescence spectra Ii(?), which are then combined to form a total correction function F(?) for a broad spectral range.
Type:
Grant
Filed:
March 23, 2010
Date of Patent:
May 24, 2011
Assignees:
Sigma-Aldrich GmbH, BAM Bundesanstalt für Materialforschung und-prüefung
Inventors:
Ute Resch-Genger, Dietmar Pfeifer, Christian Monte, Angelika Hoffmann, Pierre Nording, Bernhard Schönenberger, Katrin Hoffmann, Monika Spieles, Knut Rurack