Abstract: A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

Abstract: A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

Abstract: Adsorptive bed devices include a monolithic scaffold having a stress absorbing rigid structure and open cells filled with adsorptive beads. The monolithic scaffold restricts movement of the plurality of adsorptive beads, absorbs stress induced by a hydraulic pressure gradient along a direction of liquid flow. In one embodiment the adsorptive bed is packed into a chromatography column, and in another embodiment the adsorptive bed is sealed in a monolithic block. In another embodiment, the adsorptive bed device includes an adsorptive block, first and second planar distributors and peripheral seal.

Abstract: A cycling ultra-thin channel filtration (cUTF) method, system and module are disclosed for concentration and purification of biomolecules. In one embodiment, a cUTF system includes a cUTF module and a cycle controller to load a feed aliquot of a feed stream into the cUTF module inducing permeation of the feed stream to build a boundary layer by pressurizing the feed stream, to stop permeation and to recover a retentate aliquot in a repeating cycle.

Abstract: A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

Abstract: A lattice and distribution network for a stackable chromatography cassette comprising: a peripheral seal; at least one screen forming the lattice surrounded by the peripheral seal, each at least one screen comprising a plurality of struts in a latticed arrangement; a first internal distribution network fluidly coupled to the lattice and surrounded by the peripheral seal; a second internal distribution network disposed opposite the first internal distribution network, fluidly coupled to the lattice and surrounded by the peripheral seal; wherein a direction of fluid flow is established from the first internal distribution network through the lattice to the second internal distribution network; and wherein preferential streamlines are minimized.

Abstract: Adsorptive bed devices include a scaffold in housing having a stress absorbing rigid structure and open cells filled with adsorptive beads. The scaffold restricts movement of the plurality of adsorptive beads, absorbs stress induced by a hydraulic pressure gradient along a direction of liquid flow and transfers a portion of the induced compressive stress to the housing. In one embodiment the adsorptive bed is packed into a chromatography column, and in another embodiment the adsorptive bed is sealed in a monolithic block. In another embodiment, the adsorptive bed device includes an adsorptive block, first and second planar distributors and peripheral seal. The adsorptive media includes multiple layers of planarly cohesive media and when operated in a shallow bed configuration possesses significant tensile strength along its planar dimensions enabling it to support the hydraulic pressures that will be generated by the fluids being processed through the adsorptive devices.

Abstract: A cycling ultra-thin channel filtration (cUTF) method, system and module are disclosed for concentration and purification of biomolecules. In one embodiment, a cUTF system includes a cUTF module and a cycle controller to load a feed aliquot of a feed stream into the cUTF module inducing permeation of the feed stream to build a boundary layer by pressurizing the feed stream, to stop permeation and to recover a retentate aliquot in a repeating cycle.

Abstract: A cycling ultra-thin channel filtration (cUTF) method, system and module are disclosed for concentration and purification of biomolecules. In one embodiment, a cUTF system includes a cUTF module and a cycle controller to load a feed aliquot of a feed stream into the cUTF module inducing permeation of the feed stream to build a boundary layer by pressurizing the feed stream, to stop permeation and to recover a retentate aliquot in a repeating cycle.