Abstract: An in-line holographic microscope can be used to analyze a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. An assay using the holographic microscope for holographic particle characterization directly detect viruses, antibodies and related targets binding to the surfaces of specifically functionalized micrometer-scale colloidal probe beads. The system detects binding of targets by directly measuring associated changes in the bead's diameter without the need for downstream labeling and analysis.
Type:
Grant
Filed:
March 9, 2021
Date of Patent:
April 2, 2024
Assignees:
New York University, Spheryx, Inc
Inventors:
David G. Grier, Fook Chiong Cheong, Kaitlynn Snyder, Rushna Quddus, Lauren E. Altman, Kent Kirshenbaum
Abstract: Holographic Video Microscopy analysis of non-spherical particles is disclosed herein. Properties of the particles are determined by application of light scattering theory to holography data. Effective sphere theory is applied to provide information regarding the reflective index of a sphere that includes a target particle. Known particles may be co-dispersed with unknown particles in a medium and the holographic video microscopy is used to determine properties, such as porosity, of the unknown particles.
Type:
Grant
Filed:
December 30, 2022
Date of Patent:
March 5, 2024
Assignees:
New York University, Spheryx, Inc.
Inventors:
David G. Grier, Mary Ann Odete, Fook Chiong Cheong, Annemarie Winters, Jesse J. Elliott, Laura A. Philips
Abstract: Holographic Video Microscopy analysis of non-spherical particles is disclosed herein. Properties of the particles are determined by application of light scattering theory to holography data. Effective sphere theory is applied to provide information regarding the reflective index of a sphere that includes a target particle. Known particles may be co-dispersed with unknown particles in a medium and the holographic video microscopy is used to determine properties, such as porosity, of the unknown particles.
Type:
Application
Filed:
December 30, 2022
Publication date:
July 6, 2023
Applicants:
NEW YORK UNIVERSITY, SPHERYX, INC.
Inventors:
David G. GRIER, Mary Ann ODETE, Fook Chiong CHEONG, Annemarie WINTERS, Jesse J. ELLIOTT, Laura A. PHILIPS
Abstract: A holographic microscopy characterization (HMC) process for utilizing holographic video microscopy to provide an efficient, automated, label-free method of accurately identifying cell viability. Optical properties of a sample of cells are determined by HMC. The optical properties are compared to known samples or compared over time to observe changes in the optical properties, enabling identification of cells as viable or not viable, or as extra-cellular or degraded cellular materials.
Type:
Application
Filed:
October 28, 2022
Publication date:
May 4, 2023
Applicant:
SPHERYX, INC.
Inventors:
Mary Ann ODETE, Rostislav BOLTYANSKIY, Fook Chiong CHEONG, Laura A. PHILIPS
Abstract: Holographic Video Microscopy analysis of non-spherical particles is disclosed herein. Properties of the particles are determined by application of light scattering theory to holography data. Effective sphere theory is applied to provide information regarding the reflective index of a sphere that includes a target particle. Known particles may be co-dispersed with unknown particles in a medium and the holographic video microscopy is used to determine properties, such as porosity, of the unknown particles.
Type:
Grant
Filed:
October 23, 2020
Date of Patent:
January 3, 2023
Assignees:
New York University, Spheryx, Inc.
Inventors:
David G. Grier, Mary Ann Odete, Fook Chiong Cheong, Annemarie Winters, Jesse J. Elliott, Laura A. Philips