Patents Assigned to Stratagene
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Patent number: 7312052Abstract: The subject invention provides novel compositions containing a mixture of (a) an enzyme that possesses substantial 3?–5? exonuclease activity (b) a DNA polymerase with less 3?–5? exonuclease activity than the enzyme with substantial 3?–5? exonuclease activity. Preferably, the DNA polymerase for inclusion in the compositions are DNA polymerases that substantially lack 3?–5? exonuclease activity. A preferred embodiment of the invention is a composition comprising the Taq DNA polymerase (isolated from Thermus aquaticus) and the Pfu DNA polymerase (isolated from Pyrococcus furiosus). Another aspect of the invention is to provide methods for synthesizing polynucleotides, typically DNA, using compositions comprising an enzyme that possesses substantial 3?–5? exonuclease activity and a DNA polymerase with less 3?–5? exonuclease activity than the enzymes possessing substantial 3?–5? exonuclease activity, preferably a DNA polymerase that substantially lacks 3?–5? exonuclease activity.Type: GrantFiled: September 18, 1995Date of Patent: December 25, 2007Assignee: Stratagene CaliforniaInventors: Joseph A. Sorge, Rebecca Lynn Mullinax
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Publication number: 20070292865Abstract: The invention relates to compositions and methods for generating a signal indicative of the presence of a target nucleic acid in a sample utilizing a cleavage resistant probe.Type: ApplicationFiled: November 30, 2006Publication date: December 20, 2007Applicant: Stratagene CaliforniaInventor: Joseph Sorge
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Patent number: 7309573Abstract: The invention relates to methods and compositions for detecting a target nucleic acid. The methods include subjecting a target, template, cleavage agent, polymerase and first, second and third oligonucleotide to reaction conditions that permit: (1) formation of a duplex between the target and first oligonucleotide; (2) extension of the first oligonucleotide; (3) cleavage of a first cleavage structure; (4) formation of a second duplex with a released flap; (5) extension of the released flap and (6) cleavage of a second cleavage structure within the third oligonucleotide. A signal generated by the release of the third oligonucleotide produces a signal that is detected and indicative of the presence of the target in the sample.Type: GrantFiled: August 5, 2005Date of Patent: December 18, 2007Assignee: Stratagene CaliforniaInventor: Joseph A. Sorge
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Patent number: 7297519Abstract: The invention provides compositions containing a mixture of (a) an enzyme that possesses substantial 3?-5? exonuclease activity (b) a DNA polymerase with less 3?-5? exonuclease activity than the enzyme with substantial 3?-5? exonuclease activity. Preferably, the DNA polymerase for inclusion in the compositions are DNA polymerases that substantially lack 3?-5? exonuclease activity. A preferred embodiment of the invention is a composition comprising Taq DNA polymerase (isolated from Thermus aquaticus) and Pfu DNA polymerase (isolated from Pvrococcus furiosus). Another aspect of the invention is to provide methods for synthesizing polynucleotides using compositions comprising an enzyme that possesses substantial 3?-5? exonuclease activity and a DNA polymerase with less 3?-5? exonuclease activity than the enzymes possessing substantial 3?-5? exonuclease activity, preferably a DNA polymerase that substantially lacks 3?-5? exonuclease activity.Type: GrantFiled: December 13, 2002Date of Patent: November 20, 2007Assignee: Stratagene CaliforniaInventors: Joseph A. Sorge, Rebecca Lynn Mullinax
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Publication number: 20070259371Abstract: The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample.Type: ApplicationFiled: April 11, 2007Publication date: November 8, 2007Applicant: Stratagene CaliforniaInventors: Joseph Sorge, Ronald Davis
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Publication number: 20070248979Abstract: The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample.Type: ApplicationFiled: April 11, 2007Publication date: October 25, 2007Applicant: Stratagene CaliforniaInventors: Joseph Sorge, Ronald Davis
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Publication number: 20070248980Abstract: The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample.Type: ApplicationFiled: April 11, 2007Publication date: October 25, 2007Applicant: Stratagene CaliforniaInventors: Joseph Sorge, Ronald Davis
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Publication number: 20070231815Abstract: The present invention is directed to kits and compositions for generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising a nucleic acid polymerase lacking 5? to 3? exonuclease activity and a thermostable FEN nuclease consisting of a 5? to 3? exonuclease and/or endonuclease activity.Type: ApplicationFiled: November 29, 2006Publication date: October 4, 2007Applicant: Stratagene CaliforniaInventor: Joseph Sorge
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Patent number: 7276597Abstract: The invention relates to nucleic acid compositions for use in sequential amplification detection assays which permit the detection of a nucleic acid target in a sample.Type: GrantFiled: November 5, 2004Date of Patent: October 2, 2007Assignee: Stratagene CaliforniaInventor: Joseph A. Sorge
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Publication number: 20070212722Abstract: The invention relates to compositions and methods of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample.Type: ApplicationFiled: February 13, 2007Publication date: September 13, 2007Applicant: Stratagene CaliforniaInventor: Joseph Sorge
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Publication number: 20070212726Abstract: The invention relates to compositions and methods of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample.Type: ApplicationFiled: February 22, 2007Publication date: September 13, 2007Applicant: Stratagene CaliforniaInventor: Joseph Sorge
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Publication number: 20070212761Abstract: The invention relates to compositions and methods of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample.Type: ApplicationFiled: February 22, 2007Publication date: September 13, 2007Applicant: Stratagene CaliforniaInventor: Joseph Sorge
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Publication number: 20070212724Abstract: The invention relates to compositions and methods of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample.Type: ApplicationFiled: February 16, 2007Publication date: September 13, 2007Applicant: Stratagene CaliforniaInventor: Joseph Sorge
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Publication number: 20070212725Abstract: The invention relates to compositions and methods of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample.Type: ApplicationFiled: February 16, 2007Publication date: September 13, 2007Applicant: Stratagene CaliforniaInventor: Joseph Sorge
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Publication number: 20070207475Abstract: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.Type: ApplicationFiled: November 16, 2006Publication date: September 6, 2007Applicants: Scripps Research Institute, Medical Research Council, StratageneInventors: William Huse, Gregory Winter, Lutz Riechmann, Joseph Sorge, Richard Lerner
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Patent number: 7238478Abstract: The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.Type: GrantFiled: November 13, 2003Date of Patent: July 3, 2007Assignee: Stratagene CaliforniaInventors: Jeffrey C. Braman, Carsten-Peter Carstens, Natalia Novoradovskaya, Rajesh Bagga, Lee Scott Basehore
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Publication number: 20070148671Abstract: The present invention discloses methods of using DNA polymerase fusions at high pH in PCR, DNA sequencing and mutagenesis protocols.Type: ApplicationFiled: November 29, 2006Publication date: June 28, 2007Applicant: Stratagene CaliforniaInventor: Michael Borns
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Patent number: 7235362Abstract: The invention provides for polynucleotides and vectors comprising at least two tag sequences. In particular, preferred vectors are viral vectors. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for methods of using the polynucleotides and vectors of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.Type: GrantFiled: November 12, 2004Date of Patent: June 26, 2007Assignee: Stratagene CaliforniaInventors: Jeffrey Carl Braman, Carsten-Peter Carstens, Natalia Novoradovskaya, Rajesh Bagga, Lee Scott Basehore
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Patent number: 7189841Abstract: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.Type: GrantFiled: August 2, 2004Date of Patent: March 13, 2007Assignees: Scripps Research Institute, Medical Research Council, StratageneInventors: Richard A. Lerner, Joseph A. Sorge, Gregory P. Winter, Lutz Riechmann
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Patent number: 7189508Abstract: The invention relates to generating a signal indicative of a target nucleic acid sequence, comprising forming a complex by incubating a sample comprising a target nucleic acid sequence with a probe comprising a first and second subunit, and a binding moiety, and dissociating the first and second subunit to release the first subunit and generate a signal. The invention also relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising forming a complex by incubating a target nucleic acid sequence, an upstream primer and a probe comprising a first and second subunit, and a binding moiety. The primer is extended with a nucleic acid polymerase to displace a portion of the first subunit from the target nucleic acid strand thereby dissociating the first subunit from the second subunit to release the first subunit and generate a signal.Type: GrantFiled: July 17, 2002Date of Patent: March 13, 2007Assignee: Stratagene CaliforniaInventors: Joseph Sorge, Anne M. Whalen