Abstract: The present invention is directed to a process for increasing the free pool lysine content of individual maize seeds and for increasing the average free pool lysine content of seeds produced by a maize plant. The process, in general, comprises initiating callus from maize tissue, selecting for callus tissue resistant to S-2-aminoethyl-L-cysteine, and regenerating plants from the selected callus. In addition, the callus may be maintained before or during the selection scheme, or in place of the selection scheme.

Abstract: The invention is directed to a process for regenerating plants from corn tissue. The process comprises the steps of in vitro conditioning of the corn tissue, culturing the corn tissue on a callus induction medium to induce callus, and regenerating plants from the callus. The callus may optionally be maintained prior to regeneration and the plants may optionally be cultured on an establishment medium prior to transfer to the soil.

Abstract: The present invention relates to the regeneration of soybeans. The process comprises the steps of inducing callus and embryoid formation from tissue of a soybean plant, maturing the embryoids, germinating the embryoids, and forming roots on the germinated embryoids.

Abstract: The present invention relates to the regeneration of corn. The process comprises the steps of:(a) culturing tissue obtained from a corn plant on a first medium which comprises mineral salts, vitamins sucrose and a hormone for callus formation;(b) subculturing the calli on a second medium which comprises mineral salts, vitamins, sucrose and a hormone for callus maintenance;(c) subculturing the calli on a third medium which comprises mineral salts, vitamins, sucrose and optionally a hormone for shoot and root formation; and(d) optionally subculturing said shoots on a fourth medium which comprises mineral salts, vitamins, sucrose and optionally a hormone for plantlet maturation including additional root formation, whereby plants are obtained.

Abstract: The invention is directed to a process for regenerating plants from mature corn embryos. The process comprises the steps of culturing the mature embryo on a callus induction medium to induce callus, subculturing the callus on a maintenance medium to maintain the callus, and subculturing the maintained callus on a regeneration medium to produce plants.

Abstract: The present invention relates to the regeneration of soybeans. The process comprises the steps of:(a) culturing tissue obtained from a soybean plant on a first medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure callus and embryoid formation;(b) subculturing the embryoids on a second medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure embryoid maturation;(c) subculturing the embryoids on a third medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure shoot formation, and(e) subculturing the shoots on a fourth medium comprising mineral salts, vitamins and sucrose in an amount sufficient to ensure root formation.

Abstract: The present invention relates to the regeneration of sunflowers via organogenesis. The process comprises the steps of:(a) culturing tissue obtained from a sunflower plant on a first medium which comprises mineral salts, vitamins, amino acids, sucrose and a hormone in an amount sufficient to ensure callus formation;(b) subculturing said callus on a second medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure shoot formation, and(c) subculturing said shoot on a third medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure root formation, whereby plants are obtained.

Abstract: The present invention relates to the regeneration of sunflowers via embryogenesis and organogenesis.

Abstract: The present invention relates to the regeneration of sunflowers via embryogenesis. The process comprises the steps of:(a) culturing tissue obtained from a sunflower plant on a first medium which comprises mineral salts, vitamins, amino acids, sucrose and a hormone in an amount sufficient to ensure embryogenic callus formation;(b) subculturing said callus on a second medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure embryo formation and germination of said embryo, and(c) subculturing said embryo or said germinated embryo on a third medium which comprises mineral salts, vitamins and sucrose, whereby plants are obtained.

Abstract: The present invention relates to the regeneration of cereals including barley, corn, wheat, rice and sorghum. The process comprises the steps of:(a) culturing tissue obtained from a cereal plant on an induction medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure callus formation,(b) culturing the callus on a series of media, said series comprising at least one medium and each medium comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to insure differentiating to plantlets having shoots and roots at the completion of the series, and(c) culturing the plantlets on an establishment medium comprising mineral salts, vitamins and sucrose, whereby the plantlets are established so that they can be transplanted to soil.

Abstract: The present invention relates to the regeneration of corn. The process comprises the steps of:(a) culturing tissue obtained from a corn plant on a first medium which comprises mineral salts, vitamins sucrose and a hormone in an amount sufficient to ensure callus formation;(b) subculturing the calli on a second medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure callus maintenance;(c) subculturing the calli on a third medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure shoot and root formation; and(d) optionally subculturing said shoots on a fourth medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficent to ensure plantlet maturation including additional root formation, whereby plants are obtained.