Abstract: A two-photon stimulated emission depletion composite microscope using continuous light loss, the microscope comprising: a two-photon imaging unit (100) and an STED imaging unit (200), wherein for thicker samples, the two-photon imaging unit (100) can be used, and for regions of interest on the surface of samples, the STED super-resolution imaging unit (200) can be used. The two-photon stimulated emission depletion composite microscope using continuous light loss integrates two functions of STED imaging and two-photon imaging, so as to provide a powerful tool for cutting-edge biomedical research.

Abstract: A common beam scanning retinal imaging system comprises: a light source module (1), an adaptive optics module (2), a beam scanning module (3), a small field-of-view relay module (5), a large field-of-view relay module (6), a sight beacon module (9), a pupil monitoring module (7), a detection module (8), a control module (10) and an output module (11). The system can perform real-time correction of human eye aberration by adaptive optics technology, and realize the confocal scanning imaging function in a large field of view and the adaptive optics high-resolution imaging function in a small field of view simultaneously by the common beam synchronous scanning configuration combined with the two relay optical path structures for both the small field of view and the large field of view. The system can not only observe disease lesions in a wide range on the retina by the large field-of-view imaging, but also observe fine structures of the lesions by the small field-of-view high-resolution imaging.

Abstract: A structured illumination microscopic imaging system includes a structured illumination source. A beam shaping lens, an excitation optical filter and a dichroic mirror are provided on the emission light path of the structured illumination source in sequence. An objective lens and a sample are provided on the first optical path of the dichroic mirror in sequence. An emission optical filter, a tube lens, and a detector are provided on the second optical path of the dichroic mirror in sequence. The super-resolution microscopic images with a higher signal-to-noise ratio and higher contrast can be obtained under the premise of lowering the installation and processing precision requirements of the structured illumination microscopic imaging system. Compared to a structured light microscopic imaging system based on digital micromirror arrays or gratings, the system cost is reduced and the system stability is higher.

Abstract: A common beam scanning retinal imaging system comprises: a light source module (1), an adaptive optics module (2), a beam scanning module (3), a small field-of-view relay module (5), a large field-of-view relay module (6), a sight beacon module (9), a pupil monitoring module (7), a detection module (8), a control module (10) and an output module (11). The system can perform real-time correction of human eye aberration by adaptive optics technology, and realize the confocal scanning imaging function in a large field of view and the adaptive optics high-resolution imaging function in a small field of view simultaneously by the common beam synchronous scanning configuration combined with the two relay optical path structures for both the small field of view and the large field of view. The system can not only observe disease lesions in a wide range on the retina by the large field-of-view imaging, but also observe fine structures of the lesions by the small field-of-view high-resolution imaging.

Abstract: A two-photon stimulated emission depletion composite microscope, comprising a two-photon imaging unit (100) and an STED imaging unit (200), wherein the two-photon imaging unit (100) can be used for a relatively thick sample, and the STED super-resolution imaging unit can be used for a region of interest on a surface of a sample, and the microscope makes light spots generated by an excitation light and a depletion light after being focused by an objective lens (OL) accurately coincide in a three-dimensional distribution. The two-photon stimulated emission depletion composite microscope (10) integrates two functions of STED imaging and two-photon imaging and makes the two types of light spots generated by an excitation light and a depletion light after being focused by an objective lens accurately coincide in a three-dimensional distribution, thereby providing a powerful tool for cutting-edge biomedical research.

Abstract: The present invention discloses an aspheric lens eccentricity detecting device based on wavefront technology and a detecting method thereof. The device comprises: an upper optical fiber light source, an upper collimating objective lens, an upper light source spectroscope, an upper beam-contracting front lens, an upper beam-contracting rear lens, an upper imaging detector, an upper imaging spectroscope, an upper wavefront sensor, a lens-under-detection clamping mechanism, a lower light source spectroscope, a lower beam-contracting front lens, a lower beam-contracting rear lens, a lower imaging spectroscope, a lower wavefront sensor, a lower imaging detector, a lower collimating objective lens and a lower optical fiber light source.

Abstract: A STED super-resolution microscope capable of quick beam combination is disclosed, which includes a STED imaging unit and a beam combination test unit. The excitation light and the depletion light are accurately combined by the beam combination test unit, so that the imaging light spots of the two light beams passing through the STED imaging unit can accurately coincide with each other, thereby obtaining a better super-resolution imaging effect.

Abstract: A large field-of-view adaptive optics retinal imaging system and method with common optical path beam scanning, the system comprises: a light source module (1), an adaptive optics module (2), a beam scanning module (3), a defocus compensation module (4), a sight beacon module (6), a pupil monitoring module (7), a detection module (8), a control module (9) and an output module (10). The beam scanning module is configured in different scanning modes for carrying out different scanning imaging functions including a large field-of-view imaging function, a small field-of-view high-resolution imaging function and a large field-of-view high-resolution imaging function. The system is simple in structure, and the common optical path structure can obtain three types of retinal imaging images, which meets the requirements of different application scenarios and improves the application range of retinal imaging.

Abstract: A structured illumination microscopic imaging system includes a structured illumination source. A beam shaping lens, an excitation optical filter and a dichroic mirror are provided on the emission light path of the structured illumination source in sequence. An objective lens and a sample are provided on the first optical path of the dichroic mirror in sequence. An emission optical filter, a tube lens, and a detector are provided on the second optical path of the dichroic mirror in sequence. The super-resolution microscopic images with a higher signal-to-noise ratio and higher contrast can be obtained under the premise of lowering the installation and processing precision requirements of the structured illumination microscopic imaging system. Compared to a structured light microscopic imaging system based on digital micromirror arrays or gratings, the system cost is reduced and the system stability is higher.