Abstract: A bioreactor vessel (100) is provided with a rigid ledge projecting to a side thereof and defining fluid conduits (136a-c) between three respective externally-facing ports (132a-c) and a vessel chamber (105). A bioreactor system includes a cell culture module (10) having a receiving station (14) in which the vessel (100) is received, in use. Three fluid connection ports (314a-c) are located adjacent to the receiving station (14) and are in fluid connection with associated gas and/or liquid input lines (302a-c, 316, via a valve assembly (300).

Abstract: Apparatus (10) for separating individual sealed tubes (42) from an array has rack support (30) for supporting an array of tubes with their upper rims (43) disposed in a horizontal plane and all sealed with a sealing membrane (90), and a punch station (60) comprising a punch tool (62) with cutting edge (66). Cutting edge (66) pierces through membrane (90) from above to separate an individual sealed tube (42) from the remainder of the membrane. The tool has multiple cutting edges (66) in a linear array corresponding to a column of a tightly-packed x by y array of tubes in the rack (40) to simultaneously separate the tubes (42) of a whole column. The cutting edge (66) starts piercing membrane (90) at the corners (43a) of each tube rim and progressively shears through the membrane. Ejector pins (68) retain the separated tubes within the rack (40).

Abstract: A bioreactor outlet air conditioning system (10) has a bioreactor vessel (100) and a heat exchanger. The heat exchanger has a fluid flow path (196) from a headspace (108) in the vessel for venting air from the vessel, and a temperature control element (300) in thermal contact with the fluid flow path (196). The fluid flow path (196) may be defined by a disposable portion of the bioreactor vessel (100). A method of controlling evaporation within a bioreactor vessel (100), dependent on the required evaporation rate, adjusts the temperature of the temperature control element (300) to control the rate of evaporation of the liquid media (106) from the vessel (100), and may include monitoring fluids in the fluid flow path (196) to detect at least water content of the fluids exiting the vessel to adjust the temperature and control the rate of evaporation dependent on the detected water content levels.

Abstract: A bioreactor outlet air conditioning system (10) has a bioreactor vessel (100) and a heat exchanger. The heat exchanger has a fluid flow path (196) from a headspace (108) in the vessel for venting air from the vessel, and a temperature control element (300) in thermal contact with the fluid flow path (196). The fluid flow path (196) may be defined by a disposable portion of the bioreactor vessel (100). A method of controlling evaporation within a bioreactor vessel (100), dependent on the required evaporation rate, adjusts the temperature of the temperature control element (300) to control the rate of evaporation of the liquid media (106) from the vessel (100), and may include monitoring fluids in the fluid flow path (196) to detect at least water content of the fluids exiting the vessel to adjust the temperature and control the rate of evaporation dependent on the detected water content levels.

Abstract: Apparatus (10) for separating individual sealed tubes (42) from an array has rack support (30) for supporting an array of tubes with their upper rims (43) disposed in a horizontal plane and all sealed with a sealing membrane (90), and a punch station (60) comprising a punch tool (62) with cutting edge (66). Cutting edge (66) pierces through membrane (90) from above to separate an individual sealed tube (42) from the remainder of the membrane. The tool has multiple cutting edges (66) in a linear array corresponding to a column of a tightly-packed x by y array of tubes in the rack (40) to simultaneously separate the tubes (42) of a whole column. The cutting edge (66) starts piercing membrane (90) at the corners (43a) of each tube rim and progressively shears through the membrane. Ejector pins (68) retain the separated tubes within the rack (40).