Abstract: An improved polyacrylamide gel in a gel buffer, a system and method for conducting discontinuous sodium dodecyl sulfate electrophoresis is disclosed. The improved gel involves the use of a salt of 2-amino-2-methyl-1,3-propanediol at a pH of from 6.4 to 7.3 The improved process involves the use of the gel in combination with 2-amino-2-methyl-1,3-propanediol taurine as an electrolyte buffer at a pH of about 8.0 to 10.0.
Type:
Grant
Filed:
November 2, 1983
Date of Patent:
November 6, 1984
Assignee:
TechAmerica Group, Inc.
Inventors:
Aurora Fernandez de Castro, Jerry L. Neff
Abstract: A method for separating isoenzymes is disclosed. The method involves polyacrylamide gel electrophoresis in a buffer solution of a salt of 2-amino-2-methyl-1,3-propanediol at a pH of about 6.4 to 7.3 and an electrolyte buffer of 2-amino-2-methyl-1,3-propanediol taurine at a pH of about 8.0 to 10.0. After separation of the isoenzymes, the amount of individual isoenzymes present is determined.