Abstract: The present disclosure provides methods, device, and system for biochips manufacturing including a wafer processing apparatus such as a spin cell platform, a synthesizer, a wafer handling robot, an ultraviolet aligner, and a wafer centering device. The wafer processing apparatus uses a nozzle in a lid to disperse a solution to the surface of a wafer. Further, the wafer is positioned on top of a vacuum chuck and does not spin while the solution is dispensed over the surface of the wafer via surface tension, thereby permitting the first solution to react with a reagent on the surface. Further, when dispensing the first solution, a separation gap between the lid and the wafer is at a predetermined distance, for example, from about 20 micrometer to about 200 micrometer.

Abstract: The present disclosure relates to methods, processes and systems for enzymatic synthesis of oligonucleotide from a single-stranded, immobilized primer in the presence of a polymerase. Using the disclosed methods single-stranded oligonucleotides can be synthesized enzymatically from a single-stranded, immobilized primer in the presence of deoxyribonucleotide triphosphates or ribonucleotide triphosphates. Dideoxyribonucleotide triphosphates, deoxyribonucleotide triphosphates with reversible terminators, or ribonucleotide triphosphates with reversible terminators can be added enzymatically to the end of the primer or its extension products. According to the disclosed method, a single-stranded primer can bind to a template such that the thus-formed double-stranded structure can allow the polymerase to extend the primer at 3? end.

Abstract: The present disclosure relates to processes for suspending stretched nucleic acids over surface features. These processes can be used to prepare stretched nucleic acids that are more active in enzymatic reactions and other reactions than those laid down on a flat surface. These processes can be achieved by using a photoresist layer on top of a substrate, stretch a nucleic acid on top of the surface, and then remove part of the photoresist to form surface features that suspend the stretched nucleic acid. Furthermore, the formation of a hydrogel layer over the stretched nucleic acid and the surface features can transfer the stretched nucleic acid to the hydrogel for further reactions, including enzymatic reactions.

Abstract: The present invention provides a mutual capacitive touch panel including a first electrode layer and a second electrode layer. The first electrode layer includes a plurality of electrode strings extending along a first direction. The second electrode layer includes a plurality of electrode strips extending along a second direction, in which one of the electrode strips include a plurality of electrode portions connected in series, one of the electrode portions includes a main part and at least branch part, the main part crosses a corresponding one of the electrode strings, the branch part is connected to a side of the main part, and no branch part exists between the branch part and the outer side of the corresponding electrode string. A spacing between a side of the branch part adjacent to the outer side and the outer side is greater than twice a width of the branch part.

Abstract: The present disclosure provides methods of sequencing polynucleotides and compounds, compositions useful for sequencing of polynucleotides. The chemical compounds include nucleotides and their analogs which possess a sugar moiety comprising a cleavable chemical group capping the 3?-OH group and a base that is attached to a label through a cleavable linker comprising a disulfide bond. In addition, both the disulfide bond and the cleavable chemical group are cleavable by a chemical reagent. Furthermore, after the disulfide bond is cleaved by the chemical reagent, there is no free thiol group linked to the base of the nucleotides according to the fragmentation reaction shown below as an example. Example compounds according to the present disclosure are shown as Formula (I): wherein w is 1-5; X is O, S, or BH3; B is a nucleotide base or an analog thereof, L1-3 are linkers; and D1 is a label.

Abstract: The present disclosure relates to processes for derivatizing a surface of a substrate with a covalently bonded thin film of poly(methylsilsesquioxane)-bonded polymers as a platform for the synthesis of a biomolecule array. These processes can also be used to prepare a surface of a substrate for an in situ solid-phase synthesis of biomolecule array.

Abstract: Provided herein are methods and compositions for the sequencing of long nucleic acids, such as DNA. The methods and compositions are suited for the spatial labeling and sequencing of long nucleic acid molecules.

Abstract: A mutual capacitive touch panel includes a first electrode layer and a second electrode layer. The first electrode layer includes a plurality of first electrode series and a plurality of second electrode series. The second electrode layer includes a plurality of electrode strips, and each electrode strip crosses the first electrode series and the second electrode series. Each first electrode series includes a plurality of first electrodes and a plurality of second electrodes electrically connected with each other, and each first electrode and a corresponding one of the second electrodes are disposed abreast and form an electrode set. Each second electrode series includes a plurality of third electrodes electrically connected with each other, and each electrode set and each third electrode are arranged alternately along a direction.

Abstract: The present invention provides methods and systems for sequencing long nucleic acid fragments. In one aspect of the invention, methods, systems and reagent kits are provided for sequencing nucleic acid target sequences. Some embodiments of the methods, systems and reagent kits are particularly suitable for sequencing a large number of fragments, particularly long fragments.

Abstract: The present disclosure relates to processes for inverting oligonucleotide probes in an in situ synthesized array. These processes can be used to reverse the orientation of probes with respect to the substrate from 3?-bound to 5?-bound. These processes can also be used to reduce or eliminate the presence of truncated probe sequences from an in situ synthesized array.

Abstract: The present disclosure describes systems and methods to construct genomic DNA fragments library by stretching genomic DNA over an adapter-modified chip; using an enzyme cocktail comprising a first restriction enzyme, a ligase, and a second restriction enzyme to cut the genomic DNA into fragments and ligate the fragments to the adapters on the chip; and amplifying thus-obtained DNA by PCR amplification.

Abstract: The methods, systems, kits and reagents provided herein relate to the synchronization of dephased/prephased nucleic acid molecules during sequencing reactions. The methods, systems, kits and reagents provided herein can be utilized to improve the efficiency and accuracy of sequencing technologies.

Abstract: Provided herein are methods and compositions for analyzing epigenetic modifications of genomes. The methods and compositions are suited for complete epigenome sequencing of any modification for which an antibody or an affinity binding agent has been developed. In one aspect, provided herein is a method for analyzing epigenetic modification of a genome. In some embodiments of aspects provided herein, the method further comprises sequencing a sequencing library to generate sequence reads, and assembling the sequence reads with aid of a positional barcode sequence information. In some embodiments of aspects provided herein, the method further comprises determining a location of at least two different epigenetic modifications of the nucleic acid. Another aspect of the present disclosure provides a kit for analyzing an epigenetic modification of a nucleic acid.

Abstract: The present disclosure relates to processes for inverting oligonucleotide probes in an in situ synthesized array. These processes can be used to reverse the orientation of probes with respect to the substrate from 3?-bound to 5?-bound. These processes can also be used to reduce or eliminate the presence of truncated probe sequences from an in situ synthesized array.

Abstract: Provided herein are methods and compositions for the fabrication of patterned arrays, such as nucleotide arrays. The methods and compositions are suited for the transfer and reorientation of array components.

Abstract: Provided herein are methods and compositions for coating surfaces with polymers. The methods and compositions are suited for conducting biological reactions.

Abstract: A reagent storage and dispensing apparatus comprises a housing, storage wells disposed within an internal volume of the housing, and a valve. The housing comprises a shared outlet chamber. The wells can be selected to be in fluid communication with the shared outlet channel through individual outlet channels. The valve is coupled to the housing and is rotated to select a well to be in fluid communication with the shared outlet channel while preventing the remaining wells from being in fluid communication. As the valve is rotated, the wells and their individual outlet channels remain stationary. To dispense fluid from or draw fluid into the selected well, positive or negative pressure, respectively, is introduced into the internal volume. Pressurization of the internal volume also pressurizes the plurality of wells, though the valve allows only fluid flow for the selected well.

Abstract: The present disclosure provides methods, device, and system for wafer processing. The wafer processing apparatus uses lid dispenser to disperse at least one reagent to the surface of the wafer. Further, the wafer is positioned on top of a rotatable vacuum chuck configured to spread at least one reagent over the surface of the wafer via a centrifugal force or surface tension, thereby permitting the at least one reagent to react with an additional reagent. Further, when dispensing the at least one reagent, a separation gap between the lid dispenser and the wafer is at a predetermined distance, for example, from 50 ?m to 2 mm.

Abstract: The present invention provides methods and systems for sequencing long nucleic acid fragment. The present invention also provides a method of sequencing a target polynucleotide with fewer probes. Further, the present invention provides a method of sequencing a target polynucleotide with longer reads. Locus-specific, ligation-assisted sequencing/genotyping method and ligation-captured sequencing method are also provided in the present invention. The methods of the present invention allow low-cost, high-throughput and accurate sequencing of nucleic acids.

Abstract: An apparatus includes a carriage, camera, spray tool, and controller. The carriage is movably coupled to a track. The camera and spray tool are coupled to the carriage. The controller moves the carriage along the track away from a starting position based on a first signal from the camera, and as the carriage moves away from the starting position, discharges, through the spray tool, a solution for a first time. The controller moves the carriage towards the starting position after the solution has been discharged for the first time, and based on a second signal from the camera as the carriage moves towards the starting position and before the carriage reaches the starting position, moves the carriage away from the starting position. As the carriage moves away from the starting position, the controller discharges, through the spray tool, the solution for a second time.