Abstract: The identification of MreB as essential for bacterial chromosome segregation provides a new target for antibiotic action. The MreB function is useful in the development of screening assays for new antibiotics, which may use, for example, genetic mutants in MreB, tests of MreB mediated chromosome segregation, and the like. In one embodiment of the invention, the antibiotic is an isothiourea compound, which may comprise a polyhalogenated benzyl group, e.g. at the 4 position, the 2,4 position, etc. A pharmaceutical composition comprising an MreB targeted antibiotic as an active agent is administered to a patient suffering from a microbial infection, particularly bacterial infections.

Abstract: The present invention is directed towards an electrosurgical cutting system. The system comprises an electrically conductive blade, having an uninsulated cutting edge that is surrounded by an insulator. A source of pulsed electrical energy may be coupled to the electrically conductive blade to provide a substantially uniform and highly enhanced electric field along a cutting portion of the blade edge. The blade may have a uniform rate of erosion during use, so that both the conductive metal edge and the surrounding insulation layer erode at approximately the same rate. Also described are methods of fabricating insulated cutting electrodes, particularly blade electrodes.

Abstract: Recombinant adeno-associated viral (AAV) capsid proteins are provided. Methods for generating the recombinant adeno-associated viral capsid proteins and a library from which the capsids are selected are also provided.

Abstract: The invention relates to a method and apparatus for cooling multilevel entities such as atoms, ions or molecules as well as entities with no apparent internal structure. Cooling is achieved by coherent scattering, where the frequency of the emitted radiation exceeds the frequency of the illumination radiation. Such coherent scattering is achieved by placing the entities in a resonator containing in which the cavity length and mirror coating are selected to support a resonant radiation. The entities are illuminated with an illumination radiation whose energy is lower than that of the resonant radiation supported by the resonator by a certain detuning energy selected such that coherent scattering of resonant radiation from the entities at a higher frequency than that of the illumination radiation is promoted by the resonator. As a result of the coherent scattering energy is carried away from the entities and they are cooled.

Abstract: A handoff technique for wireless communication systems uses pattern recognition of signal strength data to anticipate handoffs and reduce the total number of handoffs in the system. A criterion for system performance is used in determining the necessity for handoff. A window of signal samples from nearby base stations constitutes a pattern vector which is classified using a probabilistic neural network or other learning machine. The use of averaged signals and the sequencing of classes allow for a small number of training vectors for the pattern classifier. Substantially increased performance requires only one training vector per class. Simulation results indicate that, for a given probability of failure, the pattern recognition based handoff technique yields fewer handoffs than the conventional hysteresis rule.

Abstract: A rapid and sensitive detection method capable of reliably distinguishing fragments identical to a defined sequence from fragments containing a single base pair mismatch relative to the defined sequence. The method employs a peptide analog antisense probe, such as a peptide nucleic acid (PNA) probe, which hybridizes with DNA to forms complexes having higher thermal stability, but greater sensitivity to base pair mismatches, than corresponding DNA/DNA complexes. The method may be used in a number of applications, including genetic screening and hybridization sequencing applications.

Abstract: Neointimal cells are shown to express high levels of an anti-apoptotic gene, bcl-x, while the medial cells of the vessel itself express only low levels. The difference in gene expression exploited to provide for selective deletion of the neointimal cells. Apoptosis is induced by administering anti-sense bcl-x oligonucleotides to the affected vessel. Apoptosis is desirable as a treatment because it does not induce inflammation, further tissue injury or reactive hyperplasia. A significant reduction in lesion size is seen after treatment.