Patents Assigned to The Government of the Republic of Singapore
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Patent number: 7105165Abstract: This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-āSā-133 Oon Strain (Methionine to Threonine) which constituent viral genome is deposited under Accession Nos. P97121501, P97121502 and P97121503 with the European Collection of Cell Culture on 15th Dec. 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than a methionine. This invention also provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. I.D. No. 1 and the purified peptide.Type: GrantFiled: January 20, 2004Date of Patent: September 12, 2006Assignee: Government of Republic of SingaporeInventors: Chong Jin Oon, Gek Keow Lim, Yi Zhao, Wei Ning Chen
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Patent number: 7037682Abstract: The present invention provides an in vitro activity assay for human hepatitis B virus (HBV) DNA polymerase, which comprises using, as the 5? oligonucleotide in PCR amplification of HBV DNA polymerase from a sample, an oligonucleotide into which has been incorporated the SP6 viral polymerase promoter, directly transcribing and translating the PCR products in the presence of a radio-labelled agent and measuring the priming of the HBV DNA polymerase. The present invention also provides the use of such an assay to assay activity of various serum samples, to screen for inhibitors of the HBV DNA polymerase and to test and/or screen potential anti-HBV drugs for their ability to inhibit DNA priming activity of human HBV DNA polymerase.Type: GrantFiled: August 28, 2002Date of Patent: May 2, 2006Assignee: Government of the Republic of SingaporeInventors: Chong Jin Oon, Wei Ning Chen, Gek Keow Lim, Ai Lin Leong
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Patent number: 7038035Abstract: This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-'?-145 Singapore Strain (Glycine to Arginine) which constituent viral genome is deposited under Accession Nos. P97121504, P97121505 and P97121506 with the European Collection of Cell Culture on 15th Dec. 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 145 of such polypeptide is an arginine rather than a glycine, and the purified peptide. This invention further provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. ID. No. 1, and the purified peptide.Type: GrantFiled: June 19, 1998Date of Patent: May 2, 2006Assignee: Government of Republic of SingaporeInventors: Chong Jin Oon, Gek Keow Lim, Ai Lin Leong, Yi Zhao, Wei Ning Chen
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Publication number: 20040209292Abstract: This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-‘S’-133 Oon Strain (Methionine to Threonine) which constituent viral genome is deposited under Accession Nos. P97121501, P97121502 and P97121503 with the European Collection of Cell Culture on 15th Dec. 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than a methionine. This invention also provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. I.D. No. 1 and the purified peptide.Type: ApplicationFiled: January 20, 2004Publication date: October 21, 2004Applicant: GOVERNMENT OF REPUBLIC OF SINGAPOREInventors: Chong Jin Oon, Gek Keow Lim, Yi Zhao, Wei Ning Chen
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Patent number: 6787142Abstract: This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-‘S’-133 Oon Strain (Methionine to Threonine) which constituent viral genome is deposited under Accession Nos. P97121501, P97121502 and P97121503 with the European Collection of Cell Culture on 15th December 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than a methionine. This invention also provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. I.D. No. 1 and the purified peptide.Type: GrantFiled: July 31, 2002Date of Patent: September 7, 2004Assignee: Government of Republic of SingaporeInventors: Chong Jin Oon, Gek Keow Lim, Yi Zhao, Wei Ning Chen
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Patent number: 6759193Abstract: Novel DNA probe sequences for detection, by polymerase chain reaction, of human hepatitis B virus surface antigen mutant 145 (Glycine to Arginine) from serum samples. As a direct application, these specific DNA probes are immobilized on solid glass supports (gene chip) for detection of human hepatitis B virus surface antigen mutant 145 (Glycine to Arginine) by fluorescence.Type: GrantFiled: July 28, 1999Date of Patent: July 6, 2004Assignee: The Government of the Republic of SingaporeInventors: Chong-Jin Oon, Wei-Ning Chen, Ai-Lin Leong, Shiuan Koh
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Patent number: 6593082Abstract: The present invention provides an in vitro activity assay for human hepatitis B virus (HBV) DNA polymerase, which comprises using, as the 5′ oligonucleotide in PCR amplification of HBV DNA polymerase from a sample, an oligonucleotide into which has been incorporated the SP6 viral polymerase promoter, directly transcribing and translating the PCR products in the presence of a radio-labelled agent and measuring the priming of the HBV DNA polymerase. The present invention also provides the use of such an assay to assay activity of various serum samples, to screen for inhibitors of the HBV DNA polymerase and to test and/or screen potential anti-HBV drugs for their ability to inhibit DNA priming activity of human HBV DNA polymerase.Type: GrantFiled: June 18, 2001Date of Patent: July 15, 2003Assignee: Government of the Republic of SingaporeInventors: Chong Jin Oon, Wei Ning Chen, Gek Keow Lim, Ai Lin Leong
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Patent number: 6558675Abstract: An isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-‘S’-133 Oon Strain (Methionine to Threonine) of Cell. Also, an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than methionine.Type: GrantFiled: April 30, 2001Date of Patent: May 6, 2003Assignee: Government of Republic of SingaporeInventors: Chong Jin Oon, Gek Keow Lim, Yi Zhao, Wei Ning Chen
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Publication number: 20030003111Abstract: This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-‘S’-133 Oon Strain (Methionine to Threonine) which constituent viral genome is deposited under Accession Nos. P97121501, P97121502 and P97121503 with the European Collection of Cell Culture on 15th December 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than a methionine. This invention also provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. I.D. No. 1 and the purified peptide.Type: ApplicationFiled: July 31, 2002Publication date: January 2, 2003Applicant: GOVERNMENT OF REPUBLIC OF SINGAPOREInventors: Chong Jin Oon, Gek Keow Lim, Yi Zhao, Wei Ning Chen