Abstract: Disclosed herein are systems and methods of obtaining a derivative Raman spectrum using an excitation or Raman pump beam whose wavelength is modulated in any suitable manner such as, for example, stochastically. Shifting the wavelength of the input excitation by a small amount in approaches like SERDS can isolate the Raman scatter from other spectral artifacts and reduce the false detection rate. For example, an input excitation sequence can be correlated with the response of an individual pixel of a detector. From this, pixels that have captured Raman scattered photons can be separated from pixels capturing non-Raman photons. These techniques can be expanded to other fields and/or types of spectroscopies that utilize a dispersive element detector with time-dependent spectral features.

Abstract: A structural electronics wireless sensor node is provided that includes layers of electronic components fabricated from patterned nanostructures embedded in an electrically conductive matrix. In some aspects, the structural electronics wireless sensor node includes a plurality of nanostructure layers that each form individual electronic components of the structural electronics wireless sensor node. In certain embodiments, the structural electronics wireless sensor node includes electronic components such as a resistor, a inductor, a capacitor, and/or an antenna.

Abstract: The invention relates to methods and products associated with in vivo enzyme profiling. In particular, biomarker nanoparticles capable of quantitatively detecting enzymatic activity in vivo are described. These nanoparticles can be used to detect in vivo enzyme activity. The invention also relates to products, kits, and databases for use in the methods of the invention.

Abstract: Provided herein, in some aspects, are methods and compositions for producing single-stranded DNA (ssDNA) having uniform length.

Abstract: Emulsion-based and micromolded (“MM”) or three dimensional printed (“3DP”) polymeric formulations for single injection of antigen, preferably releasing at two or more time periods, have been developed. Formulations are preferably formed of biocompatible, biodegradable polymers. Discrete regions encapsulating antigen, alone or in combination with other antigens, adjuvants, stabilizers, and release modifiers, are present in the formulations. Antigen is preferably present in excipient at the time of administration, or on the surface of the formulation, for immediate release, and incorporated within the formulation for release at ten to 45 days after initial release of antigen, optionally at ten to 90 day intervals for release of antigen in one or more additional time periods. Antigen may be stabilized through the use of stabilizing agents such as trehalose glass. In a preferred embodiment for immunization against polio, antigen is released at the time of administration, and two, four and six months thereafter.

Abstract: The present disclosure, at least in part, provides RNA cleavage based engineered bi-stable toggle switch utilizing the Programmable Endonucleolytic Scission-Induced Stability Tuning (PERSIST) platform. Also provided herein, are vectors encoding the engineered bi-stable toggle switch, and uses thereof.

Abstract: Engineered Streptococcus canis Cas9 (ScCas9) variants include an ScCas9 protein with its PID being the PID amino acid composition of Streptococcus pyogenes Cas9 (SpCas9-NG, an ScCas9 protein having a threonine-to-lysine substitution mutation at position 1227 in its amino acid sequence (Sc+), and an ScCas9 protein having a threonine-to-lysine substitution mutation at position 1227 and a substitution of residues ADKKLRKRSGKLATE [SEQ ID No. 4] in position 365-379 in the ScCas9 open reading frame (Sc++). Also included are CRISPR-associated DNA endonucleases with a PAM specificity of 5?-NG-3? or 5?-NNG-3? and a method of altering expression of a gene product by utilizing the engineered ScCas9 variants.

Abstract: Methods, apparatuses, and systems related to electrochemical capture of Lewis acid gases from fluid mixtures are generally described. Certain embodiments are related to electrochemical methods involving selectively removing a first Lewis acid gas from a fluid mixture containing multiple types of Lewis acid gases (e.g., a first Lewis acid gas and a second Lewis acid gas). Certain embodiments are related to electrochemical systems comprising certain types of electroactive species having certain redox states in which the species is capable of binding a first Lewis acid gas but for which binding with a second Lewis acid gas is thermodynamically and/or kinetically unfavorable. The methods, apparatuses, and systems described herein may be useful in carbon capture and pollution mitigation applications.

Abstract: Compositions, articles, and methods related to hydrogen gas sensors are generally described.

Abstract: The present description provides methods, assays and reagents useful for sequencing proteins. Sequencing proteins in a broad sense involves observing the plausible identity and order of amino acids, which is useful for sequencing single polypeptide molecules or multiple molecules of a single polypeptide. In one aspect, the methods are useful for sequencing multiple polypeptides. The methods and reagents described herein can be useful for high resolution interrogation of the proteome and enabling ultrasensitive diagnostics critical for early detection of diseases.

Abstract: Systems and methods are disclosed herein for quantitatively identifying a patient's sedation level and predicting adverse events, based on one or more capnograms or outputs from a pharmacokinetic, pharmacodynamic, or ventilatory model. A sensor measures a carbon dioxide concentration of air exhaled by a patient into a breath receiver. A processor processes the sensor data to generate a capnogram including one or more respiratory cycles, computes the outputs of pharmacokinetic, pharmacodynamic, or ventilatory models, and extracts one or more of the resulting features from the capnogram and pharmacokinetic, pharmacodynamic, or ventilatory model outputs. A multi-parameter metric is computed based on the one or more extracted features and estimates the current or predicted sedation level of the patient.

Abstract: The present disclosure provides compositions and methods for improved pre-targeted radioimmunotherapeutics (PRIT) to treat various hematological disorders, such as B cell hyperproliferative disorders and solid tumors. The disclosed compositions include bispecific antibody compositions having a first domain that specifically bind to an antigen such as CD38, BCMA, Muc1, GPRC5D, or Slam7, and a second domain that specifically binds to a radioactive ligand. Methods include administering the disclosed bispecific antibody reagent and separately administering the radioactive ligand. In some embodiments, a clearing agent is also administered. In some embodiments, the therapeutic methods comprise administering a combination of two or more bispecific antibody reagents. In some embodiments, an enhancing agent, such as ATRA, gamma secretase inhibitor, or dextramethasone, is also administered to enhance expression of the target antigen on the target cells.

Abstract: Disclosed is a laser system including a first laser and a second laser. The first laser includes a laser cavity, and a gas phase molecular gain medium disposed in the laser cavity, the gain medium having an absorption band. The second laser is a solid state laser configured to be continuously tunable, with respect to an emission wavelength of the second laser, over the absorption band of the gain medium, and the second laser is tuned to pump rotational vibrational transitions in the gain medium to achieve a rotational population inversion.

Abstract: A ruggedized adjustable mounting system is described for adjustably coupling and selectively locking a component supported in a fixture of the mount in a desired orientation. In some embodiments, the mounting system includes one or more kinematic couplings that support a fixture on a base and one or more adjustable couplings that are configured to adjust a separation distance between two opposing portions of the fixture and the base to selectively pivot the fixture about one or more axes of rotation.

Abstract: Chemical reaction devices involving acid and/or base, and related systems and methods, are generally described.

Abstract: Quantum sensors provide excellent performance combining high sensitivity with spatial resolution. Unfortunately, they can only detect signal fields at frequencies in a few accessible ranges, typically low frequencies up to the experimentally achievable control field amplitudes and a narrow window around their resonance frequencies. Fortunately, arbitrary-frequency signals can be detected by using the sensor qubit as a quantum frequency mixer, enabling a variety of sensing applications. The technique leverages nonlinear effects in periodically driven (Floquet) quantum systems to achieve quantum frequency mixing of the signal and an applied AC bias field. The frequency-mixed field can be detected using Rabi and CPMG sensing techniques with the bias field. Frequency mixing can distinguish vectorial components of an oscillating signal field, thus enabling arbitrary-frequency vector magnetometry.

Abstract: Reaction schemes involving acids and bases; reactors comprising spatially varying chemical composition gradients (e.g., spatially varying pH gradients), and associated systems and methods, are generally described.

Abstract: Systems and methods involving nanomaterial-based electrodes, such as supercapacitor and battery electrodes that can be flexible, are described.

Abstract: The systems and methods for monitoring and/or regulating thermal parameters in convective flow batteries is generally described.

Abstract: Electrochemical removal of chemical products in seawater, or other aqueous environments, resulting from increased atmospheric carbon dioxide levels is described.