Abstract: The present invention is directed to novel methods for assaying for modulators of GADD45 polypeptide activity. The invention also provides means to sensitize a proliferating cell to a DNA base-damaging agent by administration of novel inhibitors of GADD45 polypeptide activity. The invention further provides polypeptides which interfere with the ability of Cdc2/cyclin B1 complexes to cause a pause at the G2/M stage of the cell cycle in response to GADD45, and nucleic acids which encode such polypeptides.

Abstract: An endotracheal tube cleaning apparatus 10 which can be periodically inserted into the inside of an endotracheal tube 30 to shave away mucus deposits. In a preferred embodiment, this cleaning apparatus 10 comprises a flexible central tube 12 with an inflatable balloon 40 at its distal end. Affixed to the inflatable balloon are one or more shaving rings 70, each having a squared leading edge 72, to shave away mucus accumulations 60. In operation, the uninflated cleaning apparatus 10 is inserted into the endotracheal tube. The balloon 40 is then inflated by a suitable inflation device, such as a syringe 14, until the balloon's shaving rings are pressed against the inside surface of the endotracheal tube. The cleaning apparatus is then pulled out of the endotracheal tube to shave off mucus deposits.

Abstract: A vector particle (e.g., a retroviral vector particle) containing a chimeric envelope includes a receptor binding region that binds to a receptor of a target cell. The receptor of the target cell is other than the amphotropic cell receptor. The receptor binding region may be a receptor binding region of a human virus. A portion of the envelope gene may be deleted and the deleted portion is replaced with another receptor binding region or ligand. Such vector particles are targetable to a desired target cell or tissue, and may be administered directly to the desired target cell or tissue as part of a gene therapy procedure, or administered directly into the patient.