Patents Assigned to ThermoGenesis Corp.
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Patent number: 11278644Abstract: The present disclosure is directed to a method of preparing an osteogenic bone graft. The method includes introducing an anticoagulant compound into a volume of cellular material including a mononuclear cell population from the intramedullary canal of a long bone; collecting an effluent including the volume of cellular material containing the anticoagulant compound at a collection point; separating a concentrated cell fraction from the effluent, the concentrated cell fraction including the mononuclear cell population and the anticoagulant compound; and preparing an osteogenic bone graft from the concentrated cell fraction.Type: GrantFiled: July 20, 2016Date of Patent: March 22, 2022Assignees: DePuy Synthes Products, Inc., ThermoGenesis Corp.Inventors: Michael Lehmicke, Philip H. Coelho
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Patent number: 10045526Abstract: An apparatus and related method for the optimization of the cryopreservation of various types of cells, including but not limited to hematopoietic and mesenchymal stem and progenitor cells, and endothelial progenitor cells found in normal blood, placental/cord blood, bone marrow or the stromal vascular fraction of adipose tissue. The apparatus for cryopreservation of biological materials in a sterile environment comprises a cryopreservation workstation, a rigid disposable cartridge, and a freezing bag assembly. The apparatus and related method provide for the precise, temperature controlled, homogenously distributed introduction of a cryoprotectant containing dimethyl sulfoxide (DMSO) into a solution containing nucleated cells in a manner that provides a safe increase in osmotic pressure and a controlled release of exothermic heat within the cells as the DMSO penetrates the cell membrane and replaces water molecules prior to the freezing of the cells.Type: GrantFiled: September 9, 2014Date of Patent: August 14, 2018Assignee: Thermogenesis Corp.Inventor: Philip H. Coelho
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Patent number: 8511479Abstract: A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.Type: GrantFiled: January 24, 2012Date of Patent: August 20, 2013Assignee: ThermoGenesis Corp.Inventors: John R. Chapman, Vijay Kumar, Brian K. Cinquini, Phillip D. Kingsley
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Patent number: 8511480Abstract: A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.Type: GrantFiled: March 30, 2012Date of Patent: August 20, 2013Assignee: ThermoGenesis Corp.Inventors: John R. Chapman, Vijay Kumar, Brian K. Cinquini, Phillip D. Kingsley
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Patent number: 8506823Abstract: A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.Type: GrantFiled: January 26, 2012Date of Patent: August 13, 2013Assignee: ThermoGenesis Corp.Inventors: John R. Chapman, Vijay Kumar, Brian K. Cinquini, Phillip D. Kingsley
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Patent number: 8177072Abstract: A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.Type: GrantFiled: December 4, 2008Date of Patent: May 15, 2012Assignee: ThermoGenesis Corp.Inventors: John R. Chapman, Vijay Kumar, Brian K. Cinquini, Phillip D. Kingsley
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Patent number: 8167139Abstract: The invention includes compositions of stem and progenitor cells recovered from bone marrow or cord blood containing most of the viable CD34+ cells and substantially depleted of red blood cells resident in the original sample, without any xenobiotic additives to aid cell separation. The invention also includes a system and method for preparing the compositions. The system includes a bag set and a processing device, which utilizes an optical sensor, microcontroller, servo motor, accelerometer, load cell, and battery. The system and method utilize centrifugation to stratify the cells into layers and then separate and transfer the stem cells into a stem cell bag. The processing device's microcontroller receives input from the device's accelerometer, load cell and optical sensor to direct the metering valve in the bag set to open and close to permit the transfer of as many stems cells as possible with as few red cells as possible.Type: GrantFiled: July 6, 2007Date of Patent: May 1, 2012Assignee: ThermoGenesis Corp.Inventors: Philip H. Coelho, Bruce A. Baker, John R. Chapman, Junzhi Li, Prince Emmanuel, Robert S. Childers
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Patent number: 8066127Abstract: An apparatus and method for collecting whole blood and then separating it into components for subsequent use or storage. A self-contained bag set is used to collect the sample, which may then be placed into a device adapted to fit into a centrifuge for separation of components. Each component is then sequentially extracted according to density, with a sensor present in the device to control the operation of valves directing the collection of each component. The sensor may be reading one or more of the following characteristics: infrared, optics, density, weight, radioactive, fluorescence, color, magnetism, ultrasonic, capacitance wherein the characteristic is inherent in the blood and blood component or is an additive. Each component may then be separated into its own storage container. The preferred sensors include optics and weight.Type: GrantFiled: August 16, 2005Date of Patent: November 29, 2011Assignee: ThermoGenesis Corp.Inventors: Philip H. Coelho, Eric Sommer, Richard Klosinski, Jim Hobbs, Bruce Baker, Brian Malone
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Patent number: 7211191Abstract: An apparatus and method for collecting whole blood and then separating it into components for subsequent use or storage. A self-contained bag set is used to collect the sample, which may then be placed into a device adapted to fit into a centrifuge for separation of components. Each component is then sequentially extracted according to density, with a sensor present in the device to control the operation of valves directing the collection of each component. Each component may then be separated into its own storage container.Type: GrantFiled: September 30, 2004Date of Patent: May 1, 2007Assignee: ThermoGenesis Corp.Inventors: Philip H. Coelho, Eric Sommer, Richard Klosinski, Jim Hobbs
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Patent number: 7182107Abstract: A loading station having a dispensing manifold for loading syringe pairs with thrombin and adhesive and clotting proteins for use as a biological adhesive. The resulting loaded syringe pairs are compatible with a variety of biological adhesive dispensers and may be used in a surgical setting.Type: GrantFiled: December 15, 2003Date of Patent: February 27, 2007Assignee: ThermoGenesis Corp.Inventors: Eric Sommer, Philip Henry Coelho, Phil Kingsley
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Patent number: 7056722Abstract: A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component is harvested simultaneously from the clotting and adhesive proteins component from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.Type: GrantFiled: November 10, 2000Date of Patent: June 6, 2006Assignee: ThermoGenesis Corp.Inventors: Philip Henry Coelho, Phil Kingsley, Jim Brausch, James H. Godsey, Gail Rock
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Patent number: 6808675Abstract: A bag, method of manufacture and process are disclosed for the cryopreservation of thermolabile substances. The bag is characterized as having substantially uniform thickness throughout its length and height. The bag features a radiused peripheral edge wall for stress relief and to provide the constant cross-section. A peripheral flashing circumscribes the radiused edge wall and provides a suitable purchase area for sealing so that the thus formed bag is less susceptible to fracture particularly when exposed to cryogenic temperatures. The uniform thickness of the bag promulgates uniform heat transfer to and from the contents of the bag in relation to any surrounding medium at a different temperature. The bag affords more space for efficient storage and reduces heat invasion into the contents of the bag when a plurality of bags are placed with their larger planar surfaces in contact with each other.Type: GrantFiled: September 28, 2000Date of Patent: October 26, 2004Assignee: ThermoGenesis Corp.Inventors: Philip Henry Coelho, Pablo Rubinstein
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Patent number: 6679300Abstract: A loading station having a dispensing manifold for loading syringe pairs with thrombin and adhesive and clotting proteins for use as a biological adhesive. The resulting loaded syringe pairs are compatible with a variety of biological adhesive dispensers and may be used in a surgical setting.Type: GrantFiled: January 14, 2002Date of Patent: January 20, 2004Assignee: ThermoGenesis Corp.Inventors: Eric Sommer, Philip Henry Coelho, Phil Kingsley
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Patent number: 6472162Abstract: A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component and the clotting and adhesive proteins component are harvested simultaneously from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.Type: GrantFiled: June 4, 1999Date of Patent: October 29, 2002Assignee: ThermoGenesis Corp.Inventors: Philip Henry Coelho, Phil Kingsley, Jim Brausch, James H. Godsey, Gail Rock, Trista K. Madsen, Sona B. Frausto
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Patent number: 6302327Abstract: A system and method for controlled rate freezing and storage of thermolabile substances. The system includes a storage unit for receiving product stored within a bag and an overlying protective canister associated with a robotic arm and reading device which places the canister in the preserving environment. A control system, driven by a computer monitors the ingress, egress and storage location and particularized profiles of the articles being placed in storage.Type: GrantFiled: June 16, 1997Date of Patent: October 16, 2001Assignee: ThermoGenesis, Corp.Inventors: Philip H. Coelho, Pablo Rubinstein, Mark Buckley, Kevin Pelletier
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Patent number: 6274090Abstract: A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component is harvested simultaneously from the clotting and adhesive proteins component from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.Type: GrantFiled: August 5, 1998Date of Patent: August 14, 2001Assignee: ThermoGenesis Corp.Inventors: Philip Henry Coelho, Phil Kingsley, Jim Brausch, James H. Godsey, Gail Rock
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Patent number: 6232115Abstract: A bag, method of manufacture and process are disclosed for the cryopreservation of thermolabile substances. The bag is characterized as having substantially uniform thickness throughout its length and height. The bag features a radiused peripheral edge wall for stress relief and to provide the constant cross-section. A peripheral flashing circumscribes the radiused edge wall and provides a suitable purchase area for sealing so that the thus formed bag is less susceptible to fracture particularly when exposed to cryogenic temperatures. The uniform thickness of the bag promulgates uniform heat transfer to and from the contents of the bag in relation to any surrounding medium at a different temperature. The bag affords more space for efficient storage and reduces heat invasion into the contents of the bag when a plurality of bags are placed, with their larger planar surfaces, in contact with each other.Type: GrantFiled: November 25, 1997Date of Patent: May 15, 2001Assignee: ThermoGenesis Corp.Inventors: Philip Henry Coelho, Pablo Rubinstein
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Patent number: 6146124Abstract: A mold is disclosed for forming a freezable and thawable bag of uniform thickness, especially useful for cryopreservation of thermolabile substances. The mold features one or more recessed planar surfaces, including radiused peripheral walls about the recesses, a planar top surface, and means for conforming a blank sheet to the mold. Two mold halves combine to form a completed bag. The mold halves may be complimentary.Type: GrantFiled: June 25, 1996Date of Patent: November 14, 2000Assignee: ThermoGenesis Corp.Inventors: Philip Henry Coelho, Pablo Rubinstein
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Patent number: 6123696Abstract: A method and apparatus for the sequestration of components of a fluid by forcing heavier fractions of the fluid to pass beyond a yieldable partition integrally formed within a bag. Fluid fractions remain sequestered within bag compartments when forces driving the heavier fraction have been removed.Type: GrantFiled: July 16, 1998Date of Patent: September 26, 2000Assignee: ThermoGenesis Corp.Inventors: Philip Henry Coelho, Pablo Rubinstein
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Patent number: 6077447Abstract: A device for producing fibrinogen includes a platen having a surface configured for heat exchange with a container, which is adhered to the platen by device of a vacuum and heat exchange allowing both cooling and heating to occur along the boundary between the container and the platen. The platen is operatively coupled to a device of rocking the platen about a horizontal axis and the container allows scavenging of a cryoprecipitate fibrinogen from the blood product for subsequent utilization.Type: GrantFiled: January 22, 1999Date of Patent: June 20, 2000Assignee: ThermoGenesis Corp.Inventors: Philip H. Coelho, Terry L. Wolf, Curtis D. Mau, Jeffery D. Arnett, Richard F. Huyser