Patents Assigned to Third Wave Technologies, Inc.
  • Patent number: 7482118
    Abstract: The present invention provides novel cleavage agents for the cleavage and modification of nucleic acid. The cleavage agents find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, an endonuclease activity is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: February 20, 2004
    Date of Patent: January 27, 2009
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Michael W. Kaiser, Wu-Po Ma, Bruce P. Neri, Victor Lyamichev
  • Patent number: 7482127
    Abstract: The present invention provides methods, compositions, and kits for detecting the presence or absence of target sequences in a sample, where the sample also contains interfering sequences that are similar or identical to the target sequences. In particular, the present invention provides blocker oligonucleotides that at least partially inhibit the formation of invasive cleavage structures with the interfering sequences but do not substantially inhibit the formation of invasive cleavage structures with the target sequences.
    Type: Grant
    Filed: November 3, 2006
    Date of Patent: January 27, 2009
    Assignee: Third Wave Technologies, Inc.
    Inventors: Poonam Agarwal, Robert W. Kwiatowski, Jr.
  • Patent number: 7473773
    Abstract: The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.
    Type: Grant
    Filed: January 7, 2005
    Date of Patent: January 6, 2009
    Assignee: Third Wave Technologies, Inc.
    Inventors: Vecheslav A. Elagin, Scott M. Law, Bjork Hill
  • Patent number: 7462451
    Abstract: The present invention relates to compositions and methods for the preparation of modified nucleic acids. In particular, the present invention provides novel reagents and chemistries for the generation of linkers, modified phosphoramidites, and modified solid supports.
    Type: Grant
    Filed: April 26, 2005
    Date of Patent: December 9, 2008
    Assignee: Third Wave Technologies, Inc.
    Inventors: Zbigniev Skrzypczynski, Sarah R. Wayland
  • Publication number: 20080293046
    Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Application
    Filed: December 19, 2006
    Publication date: November 27, 2008
    Applicant: Third Wave Technologies, Inc.
    Inventors: Hatim Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael Kaiser, Robert W. Kwiatkowski, JR., Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
  • Publication number: 20080268455
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
    Type: Application
    Filed: April 8, 2008
    Publication date: October 30, 2008
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow
  • Publication number: 20080261220
    Abstract: The present invention relates to novel methods of producing oligonucleotides. In particular, the present invention provides an efficient, safe, and automated process for the production of large quantities of oligonucleotides.
    Type: Application
    Filed: October 29, 2007
    Publication date: October 23, 2008
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Raymond F. Cracauer, Craig Luedtke
  • Patent number: 7435390
    Abstract: The present invention relates to nucleic acid synthesizers and methods of using and modifying nucleic acid synthesizers. For example, the present invention provides highly efficient, reliable, and safe synthesizers that find use, for example, in high throughput and automated nucleic acid synthesis, as well as methods of modifying pre-existing synthesizers to improve efficiency, reliability, and safety.
    Type: Grant
    Filed: November 13, 2001
    Date of Patent: October 14, 2008
    Assignee: Third Wave Technologies, Inc.
    Inventors: Raymond F. Cracauer, Witold A. Ziarno
  • Patent number: 7432048
    Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5? nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: December 8, 2000
    Date of Patent: October 7, 2008
    Assignee: Third Wave Technologies, Inc.
    Inventors: Bruce P. Neri, Jeff G. Hall, Victor Lyamichev, Lloyd M. Smith
  • Patent number: 7429455
    Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
    Type: Grant
    Filed: September 5, 2006
    Date of Patent: September 30, 2008
    Assignee: Third Wave Technologies, Inc.
    Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Todd A. Anderson, James E. Dahlberg
  • Publication number: 20080220425
    Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for amplifying a synthetic DNA from a target nucleic acid, forming a nucleic acid cleavage structure on the synthetic DNA, and detecting cleavage of the nucleic acid cleavage structure as an indicator of the preset of the target nucleic acid.
    Type: Application
    Filed: October 28, 2007
    Publication date: September 11, 2008
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: WuPo Ma, Jorge Garces, Jeff G. Hall, Andrew A. Lukowiak
  • Publication number: 20080213767
    Abstract: A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide, for example, a polynucleotide analyte, in the presence of a 5?-nuclease under isothermal conditions. The polynucleotide analyte serves as a recognition element to enable a 5?-nuclease to cleave the oligonucleotide to provide (i) a first fragment that is substantially non-hybridizable to the polynucleotide analyte and (ii) a second fragment that lies 3? of the first fragment (in the intact oligonucleotide) and is substantially hybridizable to the polynucleotide analyte. At least a 100-fold molar excess of the first fragment and/or the second fragment are obtained relative to the molar amount of the polynucleotide analyte. The presence of the first fragment and/or the second fragment is detected, the presence thereof indicating the presence of the polynucleotide analyte.
    Type: Application
    Filed: October 9, 2007
    Publication date: September 4, 2008
    Applicant: Third Wave Technologies, Inc.
    Inventors: Linda M. Western, Samuel J. Rose, Edwin F. Ullman
  • Publication number: 20080199936
    Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Application
    Filed: October 28, 2007
    Publication date: August 21, 2008
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Victor I. Lyamichev, Michael W. Kaiser, Natasha Lyamicheva
  • Publication number: 20080187926
    Abstract: The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
    Type: Application
    Filed: October 30, 2007
    Publication date: August 7, 2008
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: James E. Dahlberg, Hatim T. Allawi, Victor Lyamichev, Bruce P. Neri, Marilyn C. Olson-Munoz, LuAnne Chehak, Sarah M. Olson
  • Publication number: 20080187919
    Abstract: The present invention provides methods, kits, and compositions related to human papillomavirus (HPV) primers, which may be used, for example, in nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays.
    Type: Application
    Filed: September 5, 2007
    Publication date: August 7, 2008
    Applicant: Third Wave Technologies, Inc.
    Inventors: Joseph J. King, Angela Hudson
  • Publication number: 20080188375
    Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5? nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Application
    Filed: October 28, 2007
    Publication date: August 7, 2008
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Bruce P. Neri, Jeff G. Hall, Victor I. Lyamichev, Lloyd M. Smith
  • Patent number: 7407782
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: April 12, 2005
    Date of Patent: August 5, 2008
    Assignee: Third Wave Technologies, Inc.
    Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann Brow, James L. Dahlberg
  • Publication number: 20080182980
    Abstract: The present invention relates to compositions and methods for the preparation of modified nucleic acids. In particular, the present invention provides novel reagents and chemistries for the generation of linkers and modified phosphoramidates.
    Type: Application
    Filed: November 20, 2007
    Publication date: July 31, 2008
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Zbigniev Skrzypczynski, Sarah R. Wayland
  • Publication number: 20080181823
    Abstract: The present invention provides plate spinner systems and devices, and methods of using such systems and devices. In preferred embodiments, the systems and devices contain one or more of the following features: a manual power generating component (e.g. a hand crank power supply); a rotor assembly composed of a rotor with brackets for securing a sample holding device such as a sample processing device or a micro-titer plate; a rotor hub that supports nearly all of the weight of the rotor assembly; a manually operated brake; and a kettle cover containing a rotor tachometer.
    Type: Application
    Filed: November 8, 2006
    Publication date: July 31, 2008
    Applicant: Third Wave Technologies, Inc.
    Inventors: Walter Iszczyszyn, Kurt Schicker, Paul Thompson
  • Publication number: 20080182254
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
    Type: Application
    Filed: October 28, 2007
    Publication date: July 31, 2008
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast