Abstract: A kit for the treatment of onychomycosis by nitric oxide, including: a) a pre-treatment part comprising a pharmaceutically acceptable acidifying agent in an amount sufficient to loosen up the superficial outer nail plate layer, b) a treatment part comprising a pharmaceutically acceptable nitrite and at least one polysaccharide or a NO eluting polymer in one container and ascorbic acid in another container in amounts sufficient to produce nitric oxide in an amount that reduces and/or eliminates the onychomycosis upon being mixed and c) at least two devices suitable to apply a) and b) at the treatment site and upon treatment secure that the treatment site is substantially sealed.