Abstract: The present invention provides non-propagating transgenic microalgae expressing at least one heterologous RNAi molecule. The RNAi-expressing non-propagating transgenic microalgae are used for oral delivery of the RNAi molecule to a target organism in its intact and functional form. The heterologous RNAi molecule, present within the microalgae, is characterized by being biologically active, exerting at least one specific effect on the organism consuming the microalgae or on a pathogen of said organism. In particular, the non-propagating transgenic microalgae are used as agents for biological control of animal and plant pests.

Abstract: Transgenic microalgae expressing at least one exogenous biologically active protein. The protein-expressing microalgae are used for the oral delivery of the biologically active protein to the target organism in its intact and functional form. The exogenous protein, expressed in algae, is characterized by being biologically active, exerting at least one specific activity having a beneficial effect on the subject consuming the algae. The transgenic microalgae are used as animal food for aquatic or land animals welfare or as food supplement for human healthcare.

Abstract: The present invention provides non-propagating transgenic microalgae expressing at least one heterologous RNAi molecule. The RNAi-expressing non-propagating transgenic microalgae are used for oral delivery of the RNAi molecule to a target organism in its intact and functional form. The heterologous RNAi molecule, present within the microalgae, is characterized by being biologically active, exerting at least one specific effect on the organism consuming the microalgae or on a pathogen of said organism. In particular, the non-propagating transgenic microalgae are used as agents for biological control of animal and plant pests.

Abstract: Provided are non-propagating transgenic microalgae expressing at least one heterologous RNAi molecule. The RNAi-expressing non-propagating transgenic microalgae are used for oral delivery of the RNAi molecule to a target organism in its intact and functional form. The heterologous RNAi molecule, present within the microalgae, is characterized by being biologically active, exerting at least one specific effect on the organism consuming the microalgae or on a pathogen of said organism. In particular, the non-propagating transgenic microalgae are used as agents for biological control of animal and plant pests.

Abstract: The present invention provides edible vaccines comprising transgenic microalgae expressing at least one exogenous antigen or an intervening organism comprising the transgenic microalgae. The antigen expressing microalgae are used for oral delivery of the antigen to a target organism in its intact and functional form. The exogenous antigen, expressed in the microalgae, is characterized by exerting at least one immunogenic response in the subject consuming the vaccine.

Abstract: Provided are edible vaccines including transgenic microalgae expressing at least one exogenous antigen or an intervening organism including the transgenic microalgae. The antigen expressing microalgae are used for oral delivery of the antigen to a target organism in its intact and functional form. The exogenous antigen, expressed in the microalgae, is characterized by exerting at least one immunogenic response in the subject consuming the vaccine.

Abstract: Provided are non-propagating transgenic microalgae expressing at least one heterologous RNAi molecule. The RNAi-expressing non-propagating transgenic microalgae are used for oral delivery of the RNAi molecule to a target organism in its intact and functional form. The heterologous RNAi molecule, present within the microalgae, is characterized by being biologically active, exerting at least one specific effect on the organism consuming the microalgae or on a pathogen of said organism. In particular, the non-propagating transgenic microalgae are used as agents for biological control of animal and plant pests.

Abstract: Transgenic microalgae expressing at least one exogenous biologically active protein. The protein-expressing microalgae are used for the oral delivery of the biologically active protein to the target organism in its intact and functional form. The exogenous protein, expressed in algae, is characterized by being biologically active, exerting at least one specific activity having a beneficial effect on the subject consuming the algae. The transgenic microalgae are used as animal food for aquatic or land animals welfare or as food supplement for human healthcare.

Abstract: Transgenic microalgae expressing at least one exogenous biologically active protein. The protein-expressing microalgae are used for the oral delivery of the biologically active protein to the target organism in its intact and functional form. The exogenous protein, expressed in algae, is characterized by being biologically active, exerting at least one specific activity having a beneficial effect on the subject consuming the algae. The transgenic microalgae are used as animal food for aquatic or land animals welfare or as food supplement for human healthcare.

Abstract: Provided are edible vaccines including transgenic microalgae expressing at least one exogenous antigen or an intervening organism including the transgenic microalgae. The antigen expressing microalgae are used for oral delivery of the antigen to a target organism in its intact and functional form. The exogenous antigen, expressed in the microalgae, is characterized by exerting at least one immunogenic response in the subject consuming the vaccine.

Abstract: A method for transforming algal or cyanobacterial cells, comprising mixing a polynucleotide for transforming the cells with the polynucleotide; performing microporation by applying a plurality of electrical pulses to the cells with a microporation apparatus; and incubating said polynucleotide with the cells after said applying said electrical pulses.

Abstract: This disclosure provides herbicide resistant algae and cyanobacteria. This disclosure also provides a method to cultivate algae and cyanobacteria in axenic cultures without contaminating species. Moreover, this disclosure provides transgenic algal and cyanobacterial cells that are capable of high production in high light intensities as typically applied in cultivation. Furthermore, a novel transformation method is provided for algal cells.

Abstract: The high-speed centrifugation heretofore required for harvesting micro algae and cyanobacteria cultured for biofuels and other co-products is a major cost constraint. Mixing algae/cyanobacteria at high-density culture with far less alkali than previously assumed is sufficient to flocculate the cells. The amount of flocculant required is a function of the logarithm of cell density, and is not a linear function of cell density as had been thought. The least expensive alkali treatments are with slaked limestone or dolomite (calcium hydroxide and magnesium hydroxides). Further water can be removed from the floc by sedimentation, low speed centrifugation, dissolved air flotation or filtration, prior to further processing to separate oil from valuable co-products.

Abstract: Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of cultivated algae or cyanobacteria to its wild type or to an undesirable, interbreeding related species, as well as preventing the establishment of the transgenic algae or cyanobacteria in natural ecosystems by suppressing the activity of the carbon concentrating mechanism.

Abstract: This disclosure provides a method to improve lysine and methionine content of algae and cyanobacteria through genetic modification in combination with modified expression of high lysine and methionine proteins as sinks for the amino acids. The method of this disclosure is specifically useful in animal feed production.

Abstract: This disclosure provides a method to reduce cell damage caused by near UV light absorption of algal or cyanobacterial cultures. The algal or cyanobacterial cells are transformed to express one or more fluorescent proteins, that absorb the harmful UV or near UV wavelengths and emits wavelengths that are photosynthetically more active. The photosynthetic pigments of the transgenic algal or cyanobacterial cell culture will then absorb the photosynthetically active light emitted by the fluorescent proteins. Accordingly the harmful effects of the UV and near UV radiation are reduced and the photosynthetic activity of the algal or cyanobacterial cells is improved.

Abstract: Algae and cyanobacteria are genetically engineered to have lower RUBISCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) content in order to grow more efficiently at elevated carbon dioxide levels while recycling industrial CO2 emissions back to products, and so as not to be able to grow outside of cultivation.

Abstract: This disclosure provides herbicide resistant algae and cyanobacteria. This disclosure also provides a method to cultivate algae and cyanobacteria in axenic cultures without contaminating species. Moreover, this disclosure provides transgenic algal and cyanobacterial cells that are capable of high production in high light intensities as typically applied in cultivation. Furthermore, a novel transformation method is provided for algal cells.

Abstract: Virus contaminations of bioreactors can cause considerable losses in the industry and prevention of such contaminations is usually a major concern, especially in continuous cultures and particularly in outdoor/uncovered operations such as ponds or “racing ponds”. Use of transgenic algae/cyanobacteria harboring introgressed virus/phage DNA fragments, cultured in these bioreactors/ponds will provide protection to a range of viruses/phages. Molecular mechanisms such as lysogeny and post transcriptional gene silencing (PTGS) are being exploited to produce protected algae/cyanobacteria with cross protective resistance against various viruses/phage, thus gaining bioreactor stability.

Abstract: Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of cultivated algae or cyanobacteria to its wild type or to an undesirable, interbreeding related species. as well as preventing the establishment of the transgenic algae or cyanobacteria in natural ecosystems.