Abstract: The present invention relates to transfected primary, secondary, and immortalized cells of vertebrate origin particularly mammalian origin, transfected with exogenous genetic material (DNA) which encodes a desired (e.g., a therapeutic) product or is itself a desired (e.g., therapeutic) product, methods by which primary, secondary and immortalized cells are transfected to include exogenous genetic material, including DNA targeting by homologous recombination, methods for the activation and amplification of endogenous cellular genes, methods by which cells useful for large-scale protein production can be obtained, methods of producing clonal cell strains or heterogenous cell strains, and methods of gene therapy in which transfected primary, secondary or immortalized cells are used.
Type:
Application
Filed:
November 18, 2002
Publication date:
August 7, 2003
Applicant:
Transkaryotic Therapies, Inc. a Delaware corporation
Inventors:
Douglas Treco, Michael W. Heartlein, Richard F. Selden
Abstract: The invention provides highly purified &agr;-Gal A, and various methods for purifying it; &agr;-Gal A preparations with altered charge and methods for making those preparations; &agr;-Gal A preparations that have an extended circulating half-life in a mammalian host, and methods for making same; and methods and dosages for administering an &agr;-Gal A preparation to a subject.
Type:
Application
Filed:
June 10, 2002
Publication date:
June 19, 2003
Applicant:
Transkaryotic Therapies, Inc., a Delaware corporation
Inventors:
Richard F. Selden, Marianne Borowski, Carol M. Kinoshita, Douglas A. Treco, Melanie D. Williams, Thomas J. Schuetz, Peter F. Daniel
Abstract: The invention provides highly purified &agr;-Gal A, and various methods for purifying it; &agr;-Gal A preparations with altered charge and methods for making those preparations; &agr;-Gal A preparations that have an extended circulating half-life in a mammalian host, and methods for making same; and methods and dosages for administering an &agr;-Gal A preparation to a subject.
Type:
Application
Filed:
June 7, 2002
Publication date:
April 24, 2003
Applicant:
Transkaryotic Therapies, Inc., a Delaware corporation
Inventors:
Richard F. Selden, Marianne Borowski, Carol M. Kinoshita, Douglas A. Treco, Melanie D. Williams, Thomas J. Schuetz, Peter F. Daniel
Abstract: Chimeric proteins useful in transporting a selected substance present in extracellular fluids, such as blood or lymph, into cells; quantitative assays for the selected substance using chimeric proteins; DNA encoding the chimeric proteins; plasmids which contain DNA encoding the chimeric proteins; mammalian cells, modified to contain DNA encoding the chimeric proteins, which express and, optionally, secrete the chimeric proteins; a method of producing the chimeric proteins; a method of isolating the chimeric proteins; a method of using the chimeric proteins to assay the selected substance; and a method of reducing extracellular levels of the selected substance through administration of the chimeric protein, which results in transport of the selected substance into cells.
Type:
Application
Filed:
January 3, 2001
Publication date:
September 27, 2001
Applicant:
Transkaryotic Therapies, Inc. Delaware Corporation
Inventors:
Michael W. Heartlein, Jeffrey F. Lemontt, Michael F. Concino