Abstract: An baculovirus artificial chromosome is disclosed which has the lef-8 gene inactivated. The baculovirus artificial chromosome allows the cloning and expression of heterologous genes in insect and mammalian cells without killing the host cells. Also disclosed is an infectious baculovirus comprising the artificial chromosome and a cell containing the chromosome. Also disclosed is an insect cell which expresses the LEF-8 gene in the absence of a baculovirus chromosome. Also disclosed is a method to express heterologous proteins in eukaryotic cells using the baculovirus artificial chromosome.

Abstract: An anchorage for a tendon that includes a sleeve having a smooth tapered interior bore and a compressible wedge disposed in the sleeve. The compressible wedge has a smooth exterior tapered surface tapering from a wider end to a narrower end and one or more interior channels for receiving a tendon. The taper angle of the compressible wedge is greater than the taper angle of the bore. Thus, upon insertion of the compressible wedge into the sleeve, the wider end of the compressible wedge forms a wedge contact with the sleeve before the narrower end forms a wedge contact with the sleeve. Preferably, the bore and wedge are conical, and the wedge is formed of several symmetrical pie shaped resilient sections. Corners of the sections abutting at the interior channel are rounded. An inner sleeve is disposed in the interior channel, with the outer diameter of the inner sleeve matching the diameter of the interior channel. The tendon is held by the inner sleeve.

Abstract: A vaccine which provides protective immunity against a bacterial pathogen containing a purified lactoferrin receptor protein is provided.

Abstract: Bacteria are incubated to form a biofilm on projections by providing a flow of liquid growth medium across projections, the direction of the flow of liquid being repeatedly changed, and an assay made of the resulting biofilm. Bacteria are incubated to form a biofilm on projections arranged in rows, with several projections in each row, while providing a flow of liquid growth medium across each row of projections, and an assay made of the resulting biofilm. Sensitivity of the biofilm to antibacterial reagent may be determined by treating the projections with antibacterial reagent before carrying out the assay, by treating each row of projections with a different antibacterial reagent, and each of the projections in a row with a different concentration of antibacterial reagent.

Abstract: A synthetic nuclease resistant antisense oligodeoxynucleotide capable of selectively modulating expression of human tumor necrosis factor-alpha by targeting exon sequences flanking donor splice sites, thereby regulating expression of TNF-.alpha. in a patient in need of such therapy is provided. In an embodiment either AS-ODN having the sequence set forth in SEQ ID No:4 or SEQ ID No:6 or a combination thereof can be used. The AS-ODN is administered either as the active ingredient in a pharmaceutical composition or by utilizing gene therapy techniques as an expression vector.

Abstract: A solid support for oligonucleotide synthesis is disclosed. The solid support has formula (1) wherein: R.sup.8 is selected from the group consisting of a substituted or unsubstituted C.sub.1 -C.sub.20 alkyl group, a substituted or unsubstituted C.sub.5 -C.sub.30 aryl group and a substituted or unsubstituted C.sub.5 -C.sub.40 alkylaryl group; X.sup.3 and X.sup.4 are the same or different and are selected from the group consisting of --O--, --S--, --S(O).sub.2 -- and --N(R.sup.12)--; R.sup.12 is selected from the group consisting of a substituted or unsubstituted C.sub.1 -C.sub.20 alkyl group, a substituted or unsubstituted C.sub.5 -C.sub.30 aryl group and a substituted or unsubstituted C.sub.5 -C.sub.40 alkylaryl group; and Y is selected from the group consisting of: --CH.sub.2 --CH.sub.2 --; --CH.sub.2 --; --CH.sub.2 --O--CH.sub.2 --; --CH.sub.2 --CH.sub.2 --CH.sub.2 --; --CH.dbd.CH--; --CH.dbd.C(CH.sub.3)--; --C(CH.sub.3).dbd.C(CH.sub.3)--; --CH.sub.2 --C(.dbd.CH.sub.2)--; and --CH.sub.2 --S--CH.sub.

Abstract: The invention provides vaccines and methods for preventing or treating intestinal protozoal infections in an animal. In particular, vaccines and methods for prevention or treatment of giardiasis are provided. The invention also encompasses methods of preparing and methods of use of novel toxins, antibodies, vaccine strains and compositions that result from or are used in these methods.

Abstract: An expression cassette useful for the secretion of a heterologous protein from eukaryotic cells comprising a DNA sequence encoding a promoter functionally linked to a DNA sequence coding for the juvenile hormone esterase gene which is linked in frame to a heterologous gene is disclosed. Also disclosed is a method of secreting heterologous proteins in eukaryotic cells.

Abstract: The invention provides novel tumor suppressor genes, methods for making and using these and related tumor suppressor genes and proteins and peptides, and nucleic acids encoding these and related tumor suppressor proteins and peptides.

Abstract: A linker arm for solid support oligonucleotide synthesis, the linker arm comprising formula (1), wherein: X.sup.1 is selected from the group consisting of --O--, --S--, --S(O).sub.2 --, --C(O)-- and --N(R.sup.12)--; R.sup.12 is selected from the group comprising hydrogen, a substituted or unsubstituted C.sub.1 -C.sub.20 alkyl group, a substituted or unsubstituted C.sub.5 -C.sub.30 aryl group and a substituted or unsubstituted C.sub.5 -C.sub.40 alkaryl group, X.sup.3 is --O-- or --N(H)--; R.sup.1, R.sup.2, R.sup.3, R.sup.4 and R.sup.5 are the same or different and are selected from the group consisting of hydrogen, halide, a substituted or unsubstituted C.sub.1 -C.sub.20 alkyl group, a substituted or unsubstituted C.sub.5 -C.sub.30 aryl group and a substituted or unsubstituted C.sub.5 -C.sub.40 alkylaryl group; n is 0, 1 or 2; and one of A' and B' is selected from the group consisting of hydrogen, halide, a substituted or unsubstituted C.sub.1 -C.sub.20 alkyl group, a substituted or unsubstituted C.sub.5 -C.

Abstract: An intermittently operating slow sand filter uses the damming of water in a raised pipe to provide a constant level of water in a slow sand filter container. The container includes a water inlet and outlet, and is partially filled with sand or other filter material. A pipe from the water outlet in the slow sand filter is raised upward to the desired level of water over the sand before descending to a water reservoir. Water backs up in the pipe and the container to the desired level above the top of the sand, even when the flow of water is intermittent. The schmutzdeuke forms at the top of the filter medium. The water level in such a manually operated slow sand filter, using fine quartz sand as the filter medium, and at ambient temperatures (about 21.degree. C.) is maintained above about 1 cm above the top of the filter medium and below about 8 cm above the top of the slow sand filter.

Abstract: Described herein is a method of expressing heterologous proteins in insect cells using an expression cassette comprising a structural gene for a heterologous protein physically attached to an insect cellular promoter and an enhancer. The cells may also express the IE-1 product. Also described herein is a method of killing insects comprising infecting the insects with a recombinant baculovirus comprising a structural gene for an incompatible protein functionally linked to an insect cellular promoter and an enhancer. The invention is also directed towards expression cassettes comprising an insect cellular promoter functionally linked to an enhancer wherein the promoter is capable of directing the expression of a heterologous protein in tissues containing the expression cassette, recombinant expression cassettes containing heterologous proteins, transplacement fragments, vectors and recombinant baculoviruses.

Abstract: The invention provides novel tumor suppressor genes, methods for making and using these and related tumor suppressor genes and proteins and peptides, and nucleic acids encoding these and related tumor suppressor proteins and peptides.

Abstract: A photocatalyst compound comprising: (i) a photocatalyst selected from the group consisting of TiO.sub.2, WO.sub.3 and mixtures thereof, the photocatalyst being substantially free of sharp, narrow band X-ray reflections at angles corresponding those of crystalline TiO.sub.2, WO.sub.3 and mixtures thereof, and (ii) a porous, crystalline, adsorbent support material. A process for producing the photocatalyst compound is also disclosed. The process comprises: (i) hydrolysing a non-ionic titanium compound to produce a colloidal suspension having an average particle size of less than about 250 .ANG.; (ii) contacting a porous, crystalline, adsorbent support material with the colloidal suspension to produce an impregnated support; and (iii) calcining the impregnated support to produce the photocatalyst compound. The photocatalyst compound is useful, in the treatment of a fluid containing an organic pollutant.

Abstract: The invention provides novel tumor suppressor genes, methods for making and using these and related tumor suppressor genes and proteins and peptides, and nucleic acids encoding these and related tumor suppressor proteins and peptides.

Abstract: The invention provides vaccines and methods for preventing or treating intestinal protozoal infections in an animal. In particular, vaccines and methods for prevention or treatment of giardiasis are provided. The invention also encompasses methods of preparing and methods of use of novel toxins, antibodies, vaccine strains and compositions that result from or are used in these methods.

Abstract: The present invention relates to systems and methods for automatically and continuously regulating the amount of mandibular displacement to an optimal value during obstructive sleep apnea treatment. Obstructive sleep apnea therapy is implemented by a device which automatically reevaluates an applied mandibular displacement and continually searches for a minimum displacement required to adequately distend a patient's pharyngeal airway. The minimum optimal displacement varies with body position, stage of sleep throughout the night, the patient's body weight, and whether alcohol or sleeping medicine has been ingested.

Abstract: The invention provides vaccines and methods for preventing or treating intestinal protozoal infections in an animal. In particular, vaccines and methods for prevention or treatment of giardiasis are provided. The invention also encompasses methods of preparing and methods of use of novel toxins, antibodies, vaccine strains and compositions that result from or are used in these methods.

Abstract: Use of cyclin D1 as a negative regulator of cell proliferation is disclosed. Overexpression of cyclin D1 blocks cell growth, while blocking cyclin D1 expression promotes cell proliferation.

Abstract: A reactive, powdered polymeric material, the polymeric material comprising polymer particles (i) having an average particle size less than about 1000 .mu.m; (ii) which are made up of a plurality of smaller particles; and (iii) having energy stored therein. A process for producing the powdered polymeric material is described. The process comprises the steps of: (i) placing polymeric material in a milling chamber equipped with impact means; (ii) accelerating the impact means to at least about 50 ml/s.sup.2 ; (iii) impacting the polymeric material with the impact means; (iv) repeatedly welding and fracturing the polymeric material; and (v) repeating Steps (ii), (iii) and (iv) until a powdered polymeric material is produced having an average particle size less than about 1000 .mu.m. During Steps (iii) and (iv), the temperature of the milling chamber is maintained below the glass transition temperature of the polymeric material.