Patents Assigned to UpFront Chromatography
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Publication number: 20170318835Abstract: The present invention relates to a method for providing an alpha-lactalbumin fraction and a beta-lactoglobulin fraction from a whey material obtained from milk, the method comprising the steps of: (i) providing the whey material; (ii) contacting the whey material with a chromatographic support allowing beta-lactoglobulin to be retained by the chromatographic support; (iii) obtaining a permeate fraction from the chromatographic support comprising the alpha-lactalbumin fraction; (iv) optionally washing the chromatographic support; and (v) obtaining a retentate fraction from the chromatographic support comprising the beta-lactoglobulin fraction; wherein the whey material provided in step (i) has been depleted, or substantially depleted from at least one whey protein, such as at least 2 whey proteins, e.g. at least 3 whey proteins.Type: ApplicationFiled: August 14, 2015Publication date: November 9, 2017Applicant: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Marie Bendix Hansen
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Publication number: 20170295821Abstract: The present invention relates to a method for separating at least one soluble protein fraction from an aggregated casein-containing material, the method comprises the steps of: (i) providing the aggregated casein-containing material; (ii) Contacting the aggregated casein-containing material with a chromatographic support allowing one or more soluble protein(s) present in the aggregated casein-containing material to be retained by the chromatographic support; (iii) Obtaining a permeate fraction from the chromatographic support comprising aggregated casein; (iv) Optionally washing the chromatographic support; (v) Subjecting the chromatographic support to at least one elution buffer obtaining at least one soluble protein fraction from the chromatographic support; and wherein the chromatographic support comprises one or more mixed-mode ligands capable of binding the soluble proteins from the aggregated casein-containing material.Type: ApplicationFiled: October 6, 2015Publication date: October 19, 2017Applicant: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Marie Bendix Hansen
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Patent number: 9765111Abstract: The invention provides a process for the separation of soy protein. The process begins with an aqueous extract or solution of soy protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of soy protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest (e.g. trypsin inhibitor (TI) protein or beta-conglycinin) are isolated by eluting them from said adsorbent resin. The invention also provides various novel protein compositions obtainable via the method of the invention.Type: GrantFiled: October 23, 2013Date of Patent: September 19, 2017Assignee: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Marie Bendix Hansen, Martin Pontoppidan
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Patent number: 9035031Abstract: The present invention relates to isolation of whey proteins and the preparation of a whey product and a whey isolate. In particular the present invention relates to the isolation of a ?-lactoglobulin product and the isolation of an ?-enriched whey protein isolate from whey obtained from an animal. The ?-enriched whey protein isolate provided by the present invention is besides from being low in ?-lactoglobulin also high in ?-lactalbumin and immunoglobulin G.Type: GrantFiled: September 29, 2009Date of Patent: May 19, 2015Assignee: UPFRONT CHROMATOGRAPHY A/SInventors: Marie Bendix Hansen, Allan Otto Fog Lihme, Malene Fog Lihme
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Patent number: 8815551Abstract: The present invention relates to a method for providing an isolated biofuel and a purified protein product from a raw material suitable for the production of the biofuel or a derivative of said raw material. The method comprises the steps of: (i) subjecting the raw material or a derivative of said raw material to at least one first treatment liberating the biofuel from the raw material or the derivative of said raw material, (ii) isolating the biofuel liberated in step (i) obtaining the isolated biofuel, (iii) subjecting the raw material or a derivative of said raw material to at least one second treatment providing a material suspension, and (iv) subjecting the material suspension from step (iii) to an expanded bed adsorption process obtaining the purified protein product.Type: GrantFiled: January 15, 2008Date of Patent: August 26, 2014Assignee: Upfront Chromatography A/SInventor: Allan Otto Fog Lihme
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Publication number: 20110218327Abstract: The present invention relates to isolation of whey proteins and the preparation of a whey product and a whey isolate. In particular the present invention relates to the isolation of a ?-lactoglobulin product and the isolation of an ?-enriched whey protein isolate from whey obtained from an animal. The ?-enriched whey protein isolate provided by the present invention is besides from being low in ?-lactoglobulin also high in ?-lactalbumin and immunoglobulin G.Type: ApplicationFiled: September 29, 2009Publication date: September 8, 2011Applicant: Upfront Chromatography A/SInventors: Marie Bendix Hansen, Allan Otto Fog Lihme, Malene Fog Lihme
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Patent number: 7956166Abstract: Thus, a primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture wherein the protein-containing mixture is selected from the group consisting of milk, milk derived products, milk derived raw materials, vegetable derived products, vegetable derived extracts, fruit derived products, fruit derived extracts, fish derived products, and fish derived extracts, said method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying said mixture to an adsorption column comprising an adsorbent, said adsorbent comprises a particle with at least one high density non-porous core, surrounded by a porous material, the adsorbent having a particle density of at least 1.5 g/ml and a mean particle size of at most 150 ?m; c) optionally washing the column; d) eluting at least one protein from the adsorbent.Type: GrantFiled: November 21, 2007Date of Patent: June 7, 2011Assignee: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Marie Bendix Hansen, Inga Vaarst Andersen, Morten Aae Olander
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Patent number: 7812138Abstract: Thus, a primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture wherein the protein-containing mixture is selected from the group consisting of milk, milk derived products, milk derived raw materials, vegetable derived products, vegetable derived extracts, fruit derived products, fruit derived extracts, fish derived products, and fish derived extracts, the method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying the mixture to an adsorption column comprising an adsorbent, the adsorbent comprises a particle with at least one high density non-porous core, surrounded by a porous material, the adsorbent having a particle density of at least 1.5 g/ml and a mean particle size of at most 150 ?m; c) optionally washing the column; d) eluting at least one protein from the adsorbent.Type: GrantFiled: May 31, 2002Date of Patent: October 12, 2010Assignee: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Marie Bendix Hansen, Inga Vaarst Andersen, Morten Aae Olander
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Patent number: 7745582Abstract: The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activated agarose matrices, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.Type: GrantFiled: October 31, 2007Date of Patent: June 29, 2010Assignee: Upfront ChromatographyInventors: Allan Otto Fog Lihme, Marie Bendix Hansen
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Patent number: 7442779Abstract: The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activated agarose matricees, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.Type: GrantFiled: April 5, 2005Date of Patent: October 28, 2008Assignee: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Marie Bendix Hansen
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Publication number: 20080182979Abstract: Thus, a primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture wherein the protein-containing mixture is selected from the group consisting of milk, milk derived products, milk derived raw materials, vegetable derived products, vegetable derived extracts, fruit derived products, fruit derived extracts, fish derived products, and fish derived extracts, said method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying said mixture to an adsorption column comprising an adsorbent, said adsorbent comprises a particle with at least one high density non-porous core, surrounded by a porous material, the adsorbent having a particle density of at least 1.5 g/ml and a mean particle size of at most 150 ?m; c) optionally washing the column; d) eluting at least one protein from the adsorbent.Type: ApplicationFiled: November 21, 2007Publication date: July 31, 2008Applicant: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Marie Bendix Hansen, Inga Vaarst Andersen, Morten Aae Olander
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Patent number: 7368141Abstract: A process for isolating lactoferrin from milk or a casein-containing component or product containing lactoferrin, comprising subjecting a precipitate of casein obtained from the milk of the casein-containing component or product, with which precipitate at least part of the milk lactoferrin is associated, to a treatment with an extraction medium whereby at least part of the lactoferrin associated with the precipitate of casein is released to the medium. When lactoferrin has been released, the medium may be subjected to different types of purification operation steps. The purification operations are selected in accordance with the purity requirements of the intended specific application. Furthermore, lactoferrin and/or a composition comprising lactoferrin according to the above-mentioned process is provided.Type: GrantFiled: March 6, 2003Date of Patent: May 6, 2008Assignee: Upfront Chromatography A/SInventor: Allan Otto Fog Lihme
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Patent number: 7226535Abstract: The invention relates to a method of distributing a liquid in the fluid bed of an up-flow or a down-flow fluid bed reactor. The invention provides efficient distribution and plug flow like fluid flow through the fluid bed where turbulence and/or back-mixing of the fluid are minimized. In accordance with the invention a fluid bed system for use in treating a fluid by contacting the fluid with a solid phase media is provided and the system includes a reactor chamber adapted to contain the solid phase media and at least one fluid distribution means adapted to distribute and/or deliver the fluid to be treated among the particles of the medium.Type: GrantFiled: September 13, 2005Date of Patent: June 5, 2007Assignee: Upfront Chromatography A/SInventors: Jurgen Hubbuch, Timothy J. Hobley, Owen R. T. Thomas, Allan Lihme, Marie B. Hansen, Morten A. Olander
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Patent number: 6977046Abstract: The invention relates to a method of distributing a liquid in the fluid bed of an up-flow or a down-flow fluid bed reactor. The invention provides efficient distribution and plug flow like fluid flow through the fluid bed where turbulence and/or back-mixing of the fluid are minimized. In accordance with the invention a fluid bed system for use in treating a fluid by contacting the fluid with a solid phase media is provided and the system includes a reactor chamber adapted to contain the solid phase media and at least one fluid distribution means adapted to distribute and/or deliver the fluid to be treated among the particles of the medium.Type: GrantFiled: May 10, 2001Date of Patent: December 20, 2005Assignee: Upfront Chromatography A/SInventors: Jurgen Hubbuch, Timothy J. Hobley, Owen R. T. Thomas, Allan Lihme, Marie B. Hansen, Morten A. Olander
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Patent number: 6919436Abstract: The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activiated agarose matricees, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.Type: GrantFiled: October 11, 2002Date of Patent: July 19, 2005Assignee: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Marie Bendix Hansen
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Patent number: 6783962Abstract: The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 &mgr;m, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.Type: GrantFiled: November 20, 2001Date of Patent: August 31, 2004Assignee: UpFront ChromatographyInventors: Morten Aae Olander, Allan Otto Fog Lihme, Timothy John Hobley, Marcos Simon, Irini Theodossiou, Owen Robert Tyrynis Thomas
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Publication number: 20030187227Abstract: The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activiated agarose matricees, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.Type: ApplicationFiled: October 11, 2002Publication date: October 2, 2003Applicant: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Marie Bendix Hansen
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Patent number: 6620326Abstract: The present application describes various improvements and further developments to the Expanded Bed Adsorption (EBA) technology. In particular, the application describes an EBA process where elution is performed in fluidized mode, i.e., in expanded bed mode, the so-called All Expanded Process. This improvement which is based on the use of controlled density particles, e.g., particles having a density of at least 1.3 g/ml, provides valuable advantages with respect to use of reduced amounts of elution buffer.Type: GrantFiled: March 12, 2001Date of Patent: September 16, 2003Assignee: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, René Oehlenschlæger, Brian A. Olsen, Elias Zafirakos
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Patent number: 6498236Abstract: The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activated agarose matricees, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.Type: GrantFiled: March 19, 1999Date of Patent: December 24, 2002Assignee: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Marie Bendix Hansen
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Patent number: 6043067Abstract: In a vertical down-flow fluid bed reactor, suspended particles in liquid proximal to an inlet in an uppermost part of the reactor are agitated to form a downward extending turbulent zone having vigorously moving particles and a non-turbulent zone distal to the inlet having essentially stationary particles in liquid below and adjoining the turbulent zone. In a vertical up-flow fluid bed reactor, an upward extending turbulent zone is formed proximal to an inlet in a lowermost part of the reactor and the non-turbulent zone is above the turbulent zone. The downward or upward extend of the turbulent zone is determined by the degree of agitation. The particles may contain an active substance and be in the form of a conglomerate of base particles having a desired density to control floatation or sedimentation. Particles in the turbulent and non-turbulent zones may be different such as having different specific gravities.Type: GrantFiled: January 8, 1998Date of Patent: March 28, 2000Assignee: Upfront Chromatography A/SInventors: Allan Otto Fog Lihme, Claus Schafer Nielsen, Thorkild Christian B.o slashed.g-Hansen