Abstract: Described are methods for identifying targets in RNA molecules based on differences in RNA secondary structure related to sequence variances between different allelic forms. Also described are methods for identifying or developing small molecules which can be used to modulate a target RNA by creation of protein-small-molecule-RNA complexes, as well as compositions which include such small molecules and methods of using those small molecules and compositions.
Type:
Grant
Filed:
September 26, 2002
Date of Patent:
October 3, 2006
Assignee:
Variagenics, Inc.
Inventors:
Ling X. Shen, Gregory L. Verdine, James P. Basilion, Vincent P. Stanton, Jr.
Abstract: The present invention relates to methods for the detection of polymorphism in polynucleotides by using hybridization of fragments of segments of a polynucleotide suspected of containing a polymorphism with an oligonucleotide having a sequence complementary to a fragment identifying the polymorphism and subsequent detection of incorporated labels in the oligonucleotide-fragment duplex.
Type:
Grant
Filed:
March 22, 2002
Date of Patent:
November 30, 2004
Assignee:
Variagenics, Inc.
Inventors:
Vince P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Gregory L. Verdine, Jeffrey Olson, Colin W Dykes
Abstract: Methods for identifying and utilizing variances in genes relating to efficacy and safety of medical therapy and other aspects of medical therapy are described, including methods for selecting an effective treatment.
Type:
Application
Filed:
March 11, 2004
Publication date:
September 2, 2004
Applicant:
Variagenics, Inc., a Delaware corporation
Abstract: The present invention relates to a method for genotyping a diploid organism by cleaving segments of two alleles such that 7-20 nucleotide fragments that contain a suspected polymorphic locus are produced and comparing the masses of those fragments.
Type:
Grant
Filed:
March 26, 2002
Date of Patent:
August 17, 2004
Assignee:
Variagenics, Inc.
Inventors:
Vincent P. Stanton, Jr., Jeffrey Olson, Jia Liu Wolfe, Martin Zillmann
Abstract: The present invention is concerned generally with the field of identifying an appropriate treatment regimen for a disease based upon genotype in mammals, particularly in humans. It is further concerned with the genetic basis of inter-patient variation in response to therapy, including drug therapy. Specifically, this invention describes the identification of gene sequence variances useful in the field of therapeutics for optimizing efficacy and safety of drug therapy. These variances may be useful during the drug development process and in guiding the optimal use of already approved compounds. DNA sequence variances in candidate genes (i.e., genes that may plausibly affect the action of a drug) are tested in clinical trials, leading to the establishment of diagnostic tests useful for improving the development of new pharmaceutical products and/or the more effective use of existing pharmaceutical products.
Abstract: Disclosed are methods and reagents for detecting nucleotide mismatches (for example, due to sequence variances) in a nucleic acid sample involving the use of a nucleic acid probe derived from a hemizygous cell. Methods for determining the haplotype of a nucleic acid sample are also disclosed. Also disclosed are methods for producing the probe and kits containing the probe.
Abstract: Methods for determing genotypes and haplotypes of genes are described. Also described are single nucleotide polymorphisms and haplotypes in the ApoE gene and methods of using that information.
Type:
Application
Filed:
November 5, 2002
Publication date:
November 27, 2003
Applicant:
Variagenics, Inc. a Delaware corporation
Inventors:
Jeffrey Olson, Martin Zillmann, Vincent P. Stanton
Abstract: The present invention is directed to novel base-modified nucleotides and methods for their use in the preparation and cleavage of modified polynucleotides.
Type:
Grant
Filed:
January 8, 2002
Date of Patent:
August 26, 2003
Assignee:
Variagenics, Inc.
Inventors:
Vincent P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Charles Allerson, Gregory L. Verdine
Abstract: The present invention relates to methods for the analysis of polynucleotides including detection of variance in nucleotide sequence without the need for full sequence determination, full sequence determination of a polynucleotide, genotyping of DNA and labeling a polynucleotide fragment during the process of cleaving it into fragments.
Type:
Grant
Filed:
September 10, 1999
Date of Patent:
May 20, 2003
Assignee:
Variagenics, Inc.
Inventors:
Vincent P. Stanton, Jr., Jia Liu Wolfe, Gregory L. Verdine
Abstract: Described are methods for identifying targets in RNA molecules based on differences in RNA secondary structure related to sequence variances between different allelic forms. Also described are methods for identifying or developing small molecules which can be used to modulate a target RNA by creation of protein-small-molecule-RNA complexes, as well as compositions which include such small molecules and methods of using those small molecules and compositions.
Type:
Application
Filed:
September 26, 2002
Publication date:
April 17, 2003
Applicant:
Variagenics, Inc., a Delaware corporation
Inventors:
Ling X. Shen, Gregory L. Verdine, James P. Basilion, Vincent P. Stanton
Abstract: The present disclosure describes the use of genetic variance information for folate transport or metabolism genes or pyrimidine transport or metabolism genes in the selection of effective methods of treatment of a disease or condition. The variance imformation is indicative of the expected response of a patient to a method of treatment. Methods of determining relevant variance information and additional methods of using such variance information are also described.
Abstract: The present invention relates to methods for the detection of polymorphism in polynucleotides by using hybridization of fragments of segments of a polynucleotide suspected of containing a polymorphism with an oligonucleotide having a sequence complementary to a fragment identifying the polymorphism and subsequent detection of incorporated labels in the oligonucleotide-fragment duplex.
Type:
Grant
Filed:
September 5, 2000
Date of Patent:
December 31, 2002
Assignee:
Variagenics, Inc.
Inventors:
Vince P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Gregory L. Verdine, Jeffrey Olson
Abstract: Methods for determining genotypes and haplotypes of genes are described. Also described are single nucleotide polymorphisms and haplotypes in the ApoE gene and methods of using that information.
Abstract: The present invention relates to a method for genotyping a diploid organism by cleaving segments of two alleles such that 7-20 nucleotide fragments that contain a suspected polymorphic locus are produced and comparing the masses of those fragments.
Type:
Application
Filed:
March 26, 2002
Publication date:
October 17, 2002
Applicant:
Variagenics, Inc.
Inventors:
Vincent P. Stanton, Jeffrey Olson, Jia Liu Wolfe, Martin Zillmann
Abstract: The present invention relates to methods for the analysis of polynucleotides including detection of variance in nucleotide sequence without the need for full sequence determination, full sequence determination of a polynucleotide, genotyping of DNA and labeling a polynucleotide fragment during the process of cleaving it into fragments.
Type:
Grant
Filed:
November 9, 2000
Date of Patent:
October 1, 2002
Assignee:
Variagenics, Inc.
Inventors:
Vincent P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Gregory L. Verdine
Abstract: Disclosed are methods for the treatment of proliferative disorders using compounds and/or environmental conditions which result in a difference in sensitivity of targeted and non-targeted cells. Certain of the methods involve the identification and use of allele-specific inhibitors of conditionally essential genes.
Type:
Application
Filed:
February 14, 2001
Publication date:
September 12, 2002
Applicant:
Variagenics, Inc., a Delaware corporation
Inventors:
David E. Housman, Fred D. Ledley, Vincent P. Stanton
Abstract: The present disclosure describes methods for the computational analysis of genetic variances in the coding and non-coding regions of particular genes.
Type:
Grant
Filed:
October 14, 1999
Date of Patent:
June 4, 2002
Assignee:
Variagenics, Inc.
Inventors:
Vincent P. Stanton, Jr., Robert Mark Adams, David Steffen
Abstract: Disclosed are methods and reagents for detecting nucleotide mismatches (for example, due to sequence variances) in a nucleic acid sample involving the use of a nucleic acid probe derived from a hemizygous cell. Methods for determining the haplotype of a nucleic acid sample are also disclosed. Also disclosed are methods for producing the probe and kits containing the probe.
Abstract: The invention provides methods for treating or identifying subjects having a neurological disease or at risk for a neurological disease by determining the presence of a variant GPIIIa and/or GPIIb allele.