Abstract: A method of fabricating a device includes fabricating conductive surfaces including one or more capture surfaces and one or more reference surfaces, attaching a first molecular wire to each capture surface, and attaching a second molecular wire to each reference surface. The first and second molecular wires include chiral oligonucleotide multiplexes having identical nucleobase sequences and opposite absolute configuration. The first molecular wire includes a first oligonucleotide strand conjugated to a first functional handle including a sulfur-containing compound, and a second oligonucleotide strand conjugated to a capture agent that interacts with a target entity. The second molecular wire includes a first oligonucleotide strand conjugated to a second functional handle including a sulfur-containing compound, and a second oligonucleotide strand conjugated to a reference compound. The first and second oligonucleotide strands of each molecular wire are complementary and have the same absolute configuration.

Abstract: An apparatus that is ?m- or nm-scale in size and can include integrated circuitry logic based on sub 10 nm SIA transistor nodes, a sensing subsystem, a deciding subsystem, an effecting subsystem, and a power harvesting system is described. The sensing subsystem can identify pathogenic entities, including disease associated cells (for example, cancer cells, autoimmune cells, or pathological microbes) or viruses. The sensing subsystem can include at least one pad constructed of an electrically conductive material and linkers attached to the at least one pad. Each linker can also be attached to a targeting agent (for example, an antibody fragment) or a reference agent. Upon the binding of a targeting agent with an entity of interest, the information is transferred to the logic circuitry, which processes the binding event information and decides whether the entity is disease associated (e.g., a disease associated cell or virus) and requires delivery of therapeutic agents or other treatment.

Abstract: Enantiomeric pairs of molecular wires comprised of oligomeric nucleic acids, wherein the oligomers of each wire possess identical nucleobase pair sequences and thus identical conductivity as between wires, are constructed and used to sense biological or chemical entities of interest at the cellular or molecular level. The oligomeric molecular wires conduct voltage inputs to sensing subsystem integrated circuitry, either from an electrostatic potential arising from a targeting agent (i.e., a capture agent) binding to an intended biological or chemical target molecule, or from an electrostatic potential associated with a reference molecule that has non-specific interactions with the environment. The chirality of the oligomers imparts selectivity to either the targeting agent or the reference molecule during assembly of the sensing subsystem.