Abstract: A variants of the TM-2-2 protein, conferring recognition of the Movement Protein (MP) of the Tomato Brown Rugose Fruit Virus (ToBRFV), and wherein said variant comprises a tyrosine (Y), a phenylalanine (F) or a tryptophan (W) at the position corresponding to tyrosine 767 of the TM-2-2 protein and at least one of the following mutations: C848R, N822C, N822F, N822M, N822Y, N822W, N825H, N825K and N825T with respect to the TM-2-2 protein, potentially in combination with a F655L mutation. The present invention also relates to genetic sequences encoding such a variant protein, preferably to a mutated Tm-2-2 gene, and to plants, especially Solanum lycopersicum plants comprising in their genome the mutated gene conferring resistance to ToBRFV. The invention is also directed to parts of these plants, as well as progeny, and to the use of these sequences for providing ToBRFV resistance.

Abstract: The invention relates to a Solanum lycopersicum plant comprising in its genome QTLs confering to the plant an improved phenotype corresponding to foliar and/or fruit tolerance and/or resistance to Tomato Brown Rugose Fruit virus, with respect to a corresponding plant devoid of said QTLs, and wherein said QTLs are chosen from those present in the genome of a plant of the seeds HAZTBRFVRES1 NCIMB accession number 42758. The QTL are preferably characterized by defined alleles of different SNPs on chromosome 6, 9 and 11. The invention is also directed to parts of these plants with improved phenotype, as well as progeny, to the use of these plants for introgressing the improved phenotype in another genetic background, as well as to different methods for obtaining tomato plants or seeds with increased foliar and/or fruit tolerance or resistance to Tomato Brown Rugose Fruit virus.

Abstract: The present invention is directed to melon plant and seed, namely of C. melo subsp. melo. which are resistant to ToLCNDV, comprising in their genome introgressed sequences from C. melo subsp. agrestis var. acidulous conferring resistance to said virus, when present homozygously. The introgressed sequences are preferably characterized by defined alleles of SNPs on chromosome 11, inter alia allele A of SNP Melon_sbg_14207_58 (SEQ ID No:9). The introgressed sequences can be chosen from those present in the genome of a plant of ToLR1 accession number NCIMB 42506. The invention is also directed to parts of these resistant plants, as well as progeny, to the use of these plants for introgressing the resistance in another genetic background, as well as to different methods for obtaining resistant melon plants or seeds.

Abstract: The present disclosure provides compositions, methods, and systems for targeted plant genome editing. In some embodiments, the present disclosure provides recombinant self-replicating RNAs derived from a plant virus vector, such as those derived from Tobacco Mosaic Virus (TMV). In some embodiments, the recombinant self-replicating RNAs direct the expression of both a CRISPR endonuclease and a guide RNA capable of directing sequence-specific binding of the CRISPR endonuclease to a target DNA in the genome of plant cell.

Abstract: A Cucumis melo plant, wherein the plant homozygously includes in its genome a mutant allele of the staygreen (sgr) gene on chromosome 9, wherein the mutant allele of the sgr gene includes at least one loss-of-function mutation in comparison to the sequence of a wild-type sgr allele (SEQ ID NO:1) and wherein the mutant allele of the sgr gene confers rind color stability to the fruits of the plant at maturity and/or during post-harvest, in comparison with an isogenic non-long shelf life (non-LSL) Cucumis melo plant which does not include the mutant allele. This further relates to parts, cells and seeds of the plants, as well as related methods and processes.

Abstract: The present invention relates to Cucumis melo (C. melo) plants with resistance to Scab disease, aphids and Powdery Mildew (PM) combined with desirable agronomic traits. The present invention also provides methods of making such plants, and to methods of detecting and/or selecting such plants.

Abstract: A Solanum lycopersicum plant including a QTL giving the plant improved resistance to Tomato Brown Rugose Fruit virus, compared to a plant without the QTL, and wherein the QTL is introgressed from S. pimpinellifolium and is to be found on chromosome 9, within the chromosomal region delimited by the SNP TO-0201220 (SEQ ID NO:1) and the SNP having SEQ ID NO:101 or on chromosome 11, within the chromosomal region delimited by markers having SEQ ID NO:102 and 115. The QTL may be chosen from those present in the genome of a plant of the seeds LVSTBRFVRES2 NCIMB accession number 43591. The QTL includes defined alleles of different SNPs on chromosome 9 or 11.

Abstract: The invention relates to a Solanum lycopersicum plant resistant to Tomato Brown Rugose Fruit virus comprising in its genome the combination of the Tm-1 resistance gene on chromosome 2, and at least one quantitative trait locus (QTL) chosen from QTL3 on chromosome 11, QTL1 on chromosome 6 and QTL2 on chromosome 9, that independently confer to the plant foliar and/or fruit tolerance to TBRFV, wherein said QTLs are present in the genome of a plant of the seeds HAZTBRFVRES1 NCIMB accession number 42758. The combination of at least one of these QTLs with the Tm-1 gene delays, reduces or inhibits the replication or multiplication of the virus in the plants of the invention. The invention is also directed to parts of these plants with TBRFV resistance phenotype, as well as progeny, to the use of these plants for introgressing the resistance in another genetic background, as well as to different methods for obtaining tomato plants or seeds with increased resistance to Tomato Brown Rugose Fruit virus.

Abstract: The invention relates to a lettuce plant (Lactuca sativa L.) which may comprise a resistance allele which confers a broad spectrum resistance to Bremia lactucae, wherein the resistance allele is located on linkage group 9, and which resistance allele is as found in the genome of plants grown from seeds of which a representative sample was deposited under accession number NCIMB 42141. The invention further provides seeds, progeny, propagation material and food products from the plant.

Abstract: The present invention is directed to a commercial tomato, namely S. lycopersicum plant, which is resistant to an arthropod pest comprising in its genome introgressed sequences from S. galapagense conferring resistance to said arthropod pest, wherein the introgressed sequences are chosen from those present in the genome of a plant of the line TUT115 NCIMB accession number 42109. The commercial tomato of the invention is preferably resistant to ToMV (Tomato Mosaic Virus). The introgressed sequences are preferably found at one or more of the loci defined by the following SNP markers: SNP solcap_snp_sl_18619 on chromosome 1 and SNP solcap_snp_sl_12348 on chromosome 1.

Abstract: The invention relates to a Solanum lycopersicum plant comprising in its genome QTLs confering to the plant an improved phenotype corresponding to foliar and/or fruit tolerance and/or resistance to Tomato Brown Rugose Fruit virus, with respect to a corresponding plant devoid of said QTLs, and wherein said QTLs are chosen from those present in the genome of a plant of the seeds HAZTBRFVRES1 NCIMB accession number 42758. The QTL are preferably characterized by defined alleles of different SNPs on chromosome 6, 9 and 11. The invention is also directed to parts of these plants with improved phenotype, as well as progeny, to the use of these plants for introgressing the improved phenotype in another genetic background, as well as to different methods for obtaining tomato plants or seeds with increased foliar and/or fruit tolerance or resistance to Tomato Brown Rugose Fruit virus.

Abstract: The present invention relates to Solanum lycopersicum (S. lycopersicum) plants with resistance to Tomato Yellow Leaf Curl Virus (TYLCV), powdery mildew (PM) and nematodes. According to the invention, the resistances are provided by coupling in cis on the same chromosome the OL4 gene conferring resistance to PM and nematodes and TY1 gene conferring resistance to TYLCV, without coupling the Mi-1 gene conferring resistance to nematodes in cis with said OL4 gene conferring resistance to PM and nematodes and TY1 gene conferring resistance to TYLCV. The genes can be present homozygously or heterozygously in the genome of the S. lycopersicum plants, and they confer resistance to TYLCV, PM and nematodes. The present invention also provides methods for making such plants, and to methods of detecting and/or selecting such plants.

Abstract: Capsicum plants produce fruits which are and remain green at maturity and/or over-maturity, and not olive green like existing evergreen varieties. The plants homozygously include in their genome a mutant allele of the cl gene encoding the Capsicum stay-green (CaSGR) protein and a mutant allele of the wt gene encoding the phytoene synthase (PSY) protein. Further, parts and cells of the plants, in particular pepper fruits are and remain green at maturity and/or over-maturity. Moreover, methods for producing the Capsicum plants and methods identify, detect and/or select the plants.

Abstract: The invention relates to a Solanum lycopersicum plant resistant to Tomato Brown Rugose Fruit virus comprising in its genome the combination of the Tm-1 resistance gene on chromosome 2, and at least one quantitative trait locus (QTL) chosen from QTL3 on chromosome 11, QTL1 on chromosome 6 and QTL2 on chromosome 9, that independently confer to the plant foliar and/or fruit tolerance to TBRFV, wherein said QTLs are present in the genome of a plant of the seeds HAZTBRFVRES1 NCIMB accession number 42758. The combination of at least one of these QTLs with the Tm-1 gene delays, reduces or inhibits the replication or multiplication of the virus in the plants of the invention. The invention is also directed to parts of these plants with TBRFV resistance phenotype, as well as progeny, to the use of these plants for introgressing the resistance in another genetic background, as well as to different methods for obtaining tomato plants or seeds with increased resistance to Tomato Brown Rugose Fruit virus.

Abstract: The invention relates to a Solanum lycopersicum plant comprising in its genome QTLs confering to the plant an improved phenotype corresponding to foliar and/or fruit tolerance and/or resistance to Tomato Brown Rugose Fruit virus, with respect to a corresponding plant devoid of said QTLs, and wherein said QTLs are chosen from those present in the genome of a plant of the seeds HAZTBRFVRES1 NCIMB accession number 42758. The QTL are preferably characterized by defined alleles of different SNPs on chromosome 6, 9 and 11. The invention is also directed to parts of these plants with improved phenotype, as well as progeny, to the use of these plants for introgressing the improved phenotype in another genetic background, as well as to different methods for obtaining tomato plants or seeds with increased foliar and/or fruit tolerance or resistance to Tomato Brown Rugose Fruit virus.

Abstract: A Cucumis sativus var. sativus plant tolerant to Tomato Leaf Curl New Delhi Virus (ToLCNDV) includes in its genome the combination of a first quantitative trait locus (QTL) QTL1 on chromosome 1 and a second QTL, QTL2 on chromosome 2, at least one of QTL1 and QTL2 being homozygous, wherein said combination confers to the plant tolerance to ToLCNDV and said QTLs on chromosomes 1 and 2 are present in the genome of the seeds of plant TOCUR6080, NCIMB accession number 43427. The QTL are preferably characterized by defined alleles of different SNPs on chromosomes 1 and 2. Parts of these plants have ToLCNDV tolerance phenotype, as well as progeny, and can be used for introgressing the tolerance in another genetic background, with different methods for obtaining cucumber plants or seeds with increased tolerance to ToLCNDV and different markers linked to the QTLs used to confer tolerance phenotype.

Abstract: The invention relates to a Solanum lycopersicum plant comprising in its genome, on chromosome 1, introgressed sequences from Solanum habrochaites, wherein said introgressed sequences confer to the plant an improved phenotype corresponding to both an increased yield and an increased amount of soluble sugars allocated to fruits (Brix*Yield), with respect to a corresponding plant devoid of said sequences, and wherein said introgressed sequences are chosen from those present in the genome of a plant of the seeds ToPATYIELD NCIMB accession number 42567. The introgressed sequences are preferably characterized by defined alleles of different SNPs. on chromosome 1, inter alia allele T of SNP IL2_3605 (SEQ ID No. 9) and/or allele A of IL2_6411 (SEQ ID No. 12).

Abstract: An isolated nucleic acid encoding Orf279 protein of amino acid sequence at least 95% identical to SEQ ID NO: 4, methods for detecting orf279 DNA, orf279 RNA or Orf279 protein and method for identifying functional Rf gene encoding a protein able to bind to orf279 RNA.

Abstract: The present invention relates to resistance to Xanthomonascampestris pv. campestris (Xcc) in cauliflower. According to the invention, the resistance is provided by DNA sequences, introgressed from a green cauliflower at specific loci in the genome of a white cauliflower. The introgressed sequences can be present homozygously or heterozygously in the genome of the white cauliflower, and they confer resistance to Xcc. The invention further relates to part of these cauliflowers, to seeds, to the progeny of these cauliflowers, and to method for producing cauliflowers resistant to Xcc.

Abstract: The present invention is directed to melon plant and seed, namely of C. melo subsp. melo, which are resistant to ToLCNDV, comprising in their genome introgressed sequences from C. melo subsp. agrestis var. acidulous conferring resistance to said virus, when present homozygously. The introgressed sequences are preferably characterized by defined alleles of SNPs on chromosome 11, inter alia allele A of SNP Melon_sbg_14207_58 (SEQ ID No:9). The introgressed sequences can be chosen from those present in the genome of a plant of ToLR1 accession number NCIMB 42506. The invention is also directed to parts of these resistant plants, as well as progeny, to the use of these plants for introgressing the resistance in another genetic background, as well as to different methods for obtaining resistant melon plants or seeds.