Abstract: An alignment guide for hypodermic syringes for facilitating the withdrawal of fluid samples from one or more bottles. The guide assures perpendicular puncturing of a serum bottle septum and provides for easy disassembly for cleaning. The syringe alignment guide includes an inner tube concentrically disposed inside of a tubular housing. The inner tube has first and second ends, and an internal bore for receiving a hypodermic syringe barrel. The first end of the inner tube includes a means for mounting a hypodermic syringe and a means for removably securing the inner tube within the tubular housing. The second end of the inner tube includes a means for mounting a fluid-containing bottle which extends co-axially from the second end of the inner tube and comprises a plurality of protrusions.