Abstract: Method for isolating and purifying nucleic acids and/or oligonucleotides from a biological sample, the use of the isolated or purified nucleic acid and/or oligonucleotide for transfecting cells and also for the production of an agent for the treatment of genetic disorders, a composition suitable for the isolation or purification method and also the use of potassium acetate and a silica gel-like support material for isolating endotoxin-free nucleic acids and/or oligonucleotides or nucleic acids and/or oligonucleotides with reduced endotoxin content.
Abstract: The present invention relates to a method for screening a collection of nucleic acid molecules for a desired property of the nucleic acid or of a (poly)peptide encoded thereof, comprising the steps (a) automated picking of the cell collection containing the collection of nucleic acid molecules with a first robot; (b) automated lysis of the cells with a second robot; (c) automated separation of the cell DNA from the cell debris with a second robot; (d) optionally automated separation of endotoxins from the DNA with the second robot if the cells are bacteria; (e) automated transfection of the cells with the DNA obtained in step (c) or, if the cells are bacteria, with the DNA obtained in step (d) with a third robot; and (f) automated screening for the desired property with a fourth robot.
Type:
Application
Filed:
February 5, 2004
Publication date:
December 30, 2004
Applicant:
Xantos Biomedicine AG
Inventors:
Ulrich Pessara, Michael Kazinski, Johannes Gorl