Abstract: A process for obtaining a pure preparation of an apo- form of an [Fe2S2] ferredoxin is provided. The process comprising, applying a solution comprising a holo- form of the [Fe2S2] ferredoxin on an anion exchange column; and eluting the [Fe2S2] ferredoxin from the anion exchange column with a solution comprising a salt, thereby obtaining the pure preparation of the apo- form of the [Fe2S2] ferredoxin. Also provided are pure preparations of apo-ferredoxin as well as methods of using same.
Type:
Application
Filed:
December 18, 2007
Publication date:
June 10, 2010
Applicant:
Yissum Research Development Company of the Hebrew University of Jeruslem Ltd.