Abstract: A process for the production of micro-organisms in which a methane-utilising micro-organism is grown under aerobic conditions in the presence of (a) one or more methanol-utilising micro-organisms which is/are capable of metabolising methanol produced by the growing methane-utilising micro-organisms, and (b) one or more non-methylotropic micro-organisms which is/are capable of metabolising organic substances produced by the methane and/or methanol-utilising micro-organisms.
Type:
Grant
Filed:
January 6, 1975
Date of Patent:
December 7, 1976
Assignee:
Shell Oil Company
Inventors:
David E. F. Harrison, John H. Harwood, Barry N. Herbert
Abstract: The invention provides a method of enzymatic manufacturing of a starch conversion product having a high maltose content, using as a starting material a solution of a substrate, the method comprising bringing the substrate solution into contact with a matrix to which have been coupled both an .alpha.-1,6-glucosidase and an .alpha.-1,4-glucosidase having .beta.-amylase activity.
Abstract: A method for producing a syrup from which a soft drink containing cow's milk and having stable white turbidity resembling that of cow's milk is produced is disclosed, wherein the syrup is produced by mixing as basal ingredients cow's milk, sugar and an edible acid under a specific condition without use of any stabilizer for the suspension of milk protein.
Abstract: A new microorganism, preliminarily called Methylomonas methanolica NRRL B-5458, is cultivated in a nutrient media containing methanol as sole source of carbon, the biomass being obtained containing protein of high quality and with an improved yield.
Abstract: A process for the determination of creatine phosphokinase MB-isoenzyme (CPK-MB) level in serum in which CPK is determined before and after MB-activation with 1,4-dimercapto-2,3-dihydroxy butane or 2-mercaptoethanol, preferably in an assay system using the ATP/ADP conversion. The difference in the two values found is due to the CPK-MB present. The invention is of particular utility in the diagnosis and treatment of myocardial damage.
Type:
Grant
Filed:
September 26, 1975
Date of Patent:
November 30, 1976
Assignee:
Calbiochem
Inventors:
Parinam Srinivasa Rao, James J. Lukes, Hiltrud S. Mueller
Abstract: Improved method for producing pyruvic acid by fermentation of pyruvic acid producing mutants of the strain Candida lipolytica which require methionine and thiamine for growth.
Abstract: In the salting of vegetables, such as cucumbers, control of fermentation using brine acidity as the monitor and continually and rapidly determining brine acidity with tris(hydroxymethyl)aminomethane as a standard.
Type:
Grant
Filed:
May 11, 1972
Date of Patent:
November 23, 1976
Assignee:
The United States of America as represented by the Department of Agriculture
Inventors:
Thomas A. Bell, John L. Etchells, Raymond E. Kelling, James L. Olsen
Abstract: A texturized protein is formed by a process wherein a cereal grain protein capable of forming microfibrillar aggregates is first dissolved in water, and the pH of the solution is adjusted to 5.0-6.0. Then, the ionic strength of the solution is adjusted to 0.004-0.010 to aggregate the protein molecules into microfibrils, which are subsequently aligned in a parallel arrangement by application of a unidirectional shear thereto. Finally, an oscillating shear is applied to the aligned microfibrils, causing them to collide and thereby become texturized.
Type:
Grant
Filed:
January 15, 1976
Date of Patent:
November 23, 1976
Assignee:
The United States of America as represented by the Secretary of Agriculture
Abstract: A method of making new derivatives of streptomycin by mutational biosynthesis. A mutant of Streptomyces griseus makes streptomycin production dependent on streptidine addition.A new antibiotic, deoxystreptomycin, is disclosed.
Abstract: The enhancement of foodstuffs is effected by the addition of a small but effective amount of a flavor modifying compound from those compounds having the formula: ##SPC1##Wherein R is hydrogen, methyl, ethyl, propyl, butenyl, propenyl and n is 1 or 2; and ##SPC2##Wherein R.sub.1 is hydrogen or methyl; R.sub.2 is methyl, propyl, isopropyl or furfuryl; and n is 0, 1, or 2 with the provision that if R.sub.1 is hydrogen and n is 1, R.sub.2 is neither methyl nor furfuryl; and ##SPC3##Wherein R is methyl or acetyl.
Type:
Grant
Filed:
January 23, 1975
Date of Patent:
November 23, 1976
Assignee:
Firmenich & Cie
Inventors:
Max Winter, Fritz Gautschi, Ivon Flament, Max Stoll, Irving M. Goldman
Abstract: The invention relates to a new and useful antibacterial substance which is of the formula ##EQU1## and to processes for its production and recovery. The invention embraces this antibacterial agent and its salts as crude concentrates, as purified solids and in pure crystalline forms. This antibiotic of the Formula I is effective in inhibiting the growth of gram positive bacteria. The compound of the Formula I is prepared by cultivating a strain of Streptomyces sp. X-1092 in an aqueous carbohydrate solution containing a nitrogenous nutrient under submerged aerobic conditions until substantial activity versus Gram-positive bacteria is imparted to said solution and then recovering said compound of the Formula I from said solution.
Abstract: In a method for producing sucrose from beets or beet molasses by the deionization process using ion-exchange resins or the saccharate process or in a method for producing sucrose from beets without resorting to either of the two processes mentioned above, .alpha.-galactosidase is allowed to act upon the sugar solution, while in process, so as to hydrolyze the raffinose contained therein into sucrose and galactose. The raffinose-hydrolyzed sugar solution is returned to the process following the stage at which the sugar solution is withdrawn. Thereafter, the sugar solution is treated in the fixed sequence to effect the recovery of sucrose contained in the sugar solution.
Type:
Grant
Filed:
May 22, 1974
Date of Patent:
November 16, 1976
Assignee:
Agency of Industrial Science & Technology
Abstract: A new antibiotic, designated as antibiotic 1745A/X, is produced by the fermentation of the known microorganism Streptomyces antibioticus, ATCC 11891, using the novel compound erythronolide A oxime as the substrate. This new antibiotic is useful as an antibacterial agent.
Type:
Grant
Filed:
July 23, 1975
Date of Patent:
November 16, 1976
Assignee:
Hoffmann-La Roche Inc.
Inventors:
Richard Wightman Kierstead, Ronald Andrew Le Mahieu, David Pruess
Abstract: This disclosure relates to an improved medium for growing organisms (preferably Actinoplanes missouriensis) which produce glucose isomerase. Use of the medium results in increased yields of enzyme in shorter fermentation times. The improved medium contains molasses, corn steep liquor, and an inorganic nitrogen salt.
Abstract: A microorganism which simultaneously produces .beta.-amylase and dextrin .alpha.-1,6-glucosidase is cultured. By use of the enzymes thus produced, starch is directly hydrolyzed into maltose in a high yield.
Type:
Grant
Filed:
January 6, 1975
Date of Patent:
November 16, 1976
Assignee:
Agency of Industrial Science & Technology
Abstract: A process for the enzymatic isomerization of glucose to fructose where the glucose isomerase is combined with magnesium or magnesium and cobalt ions which have been fixed to a cation exchange resin. The glucose isomerase remains combined with the metal ions during the isomerization. The use of the instant complex prolongs the working life of the enzyme and minimizes the need for conventional pH control.
Abstract: Alcohol is manufactured from cellulosic materials by a one-step process involving the simultaneous reaction of a cellulosic material, a cellulase and an alcohol-producing microorganism.
Type:
Grant
Filed:
September 8, 1975
Date of Patent:
November 9, 1976
Assignee:
Bio Research Center Company Limited
Inventors:
William Frederick Gauss, Shuzo Suzuki, Motoyoshi Takagi
Abstract: An improved composition and method for detecting fibrinogen, fibrinogen split products and/or fibrin split products in blood comprises utilizing killed, dyed Staphylococcus aureus cells which can be prepared by either of two methods. In the first method, Staphylococcus aureus organisms are incubated in a nutrient medium containing a polyalkylene glycol having a molecular weight of from 1000 to 5000 to which is added triphenyltetrazolium chloride; the growing organisms reduce the triphenyltetrazolium chloride to triphenylformazan which imparts coloration to the cells; the dyed Staphylococcus aureus cells are killed and substantially all untrapped dye is removed. In an alternate method, a suspension of Fast Black Salt K is added to a suspension of killed Staphylococcus aureus cells, and the dyed cells which result are washed to remove substantially all unfixed dye. The killed, dyed Staphylococcus aureus cells prepared by either method are suspended in an imidazole buffer to maintain a pH of 7.4.
Type:
Grant
Filed:
March 7, 1975
Date of Patent:
November 9, 1976
Assignee:
Warner-Lambert Company
Inventors:
James R. Butler, Walter E. Jacobson, Donald Paul Kronish, James E. Turner, Lee S. Zuriff
Abstract: In the enzymatic hydrolysis of cellulose to obtain water-soluble sugars, the enzyme source is the aqueous culture mass obtained in an enzyme preparation step by cultivating in an aqueous nutrient medium in the presence of a cellulosic material a cellulolytic microorganism capable of elaborating a cellulolytic enzyme complex which can degrade native cellulose. No separation is made of any component of the culture mass. Such use of the culture mass as the enzyme source not only eliminates processing steps to separate the enzyme, but also results in increased hydrolysis rates and yields of the desired water-soluble sugars in the hydrolysis of cellulose.
Abstract: This application involves a process of reducing nucleic acid (RNA) in yeast products by heating the protein containing portion of the yeast to over 100.degree. C. for 10 seconds to 60 minutes at a pH of about 6 to about 8. A novel yeast cell wall low RNA protein product is disclosed as is a low RNA protein isolate.