Abstract: Method and apparatus are described for purifying and separating biopolymers from a homogenized biological milieu using a pipette tip. The biopolymers are complexed with first magnetic particles. A magnet is applied to the pipette tip to attract the biopolymer/magnetic particle complex to the inner surface of the pipette tip adjacent to the magnet for separation from the biological milieu. The magnet is removed and the magnetic particles dissociated and removed from the biopolymers. Second magnetic particles are bound to the biopolymers. The magnet is replaced against the exterior surface of the pipette tip, thereby attracting the bound biopolymers to the inner surface of the pipette tip adjacent to the magnet to allow separation from impurities. The biopolymers are then labeled for detection, which may include detecting the concentration and identity of the biopolymer.

Abstract: Bioavailability of peptide active agents to be administered orally is enhanced by a pharmaceutical composition providing targeted release of the peptide to the intestine by virtue of an acid-resistant protective vehicle which transports components of the invention through the stomach. The composition includes an absorption enhancer and a sufficient amount of a pH-lowering agent to lower local intestinal pH. All components are released together into the intestine with the peptide.

Abstract: The invention includes a vaccine and sera for treatment of Mystery Swine Disease (MSD), a method for producing the vaccine, methods for diagnosis of MSD, a viral agent that will mimic "mystery swine disease" and antibodies to the viral agent useful in diagnosis and treatment of MSD. The serum contains mammalian antibodies which are effective in treating MSD.

Abstract: A method for preventing canine coronavirus in dogs is disclosed which comprises administering to a dog a live or inactivated vaccine prepared from transmissible gastroenteritis virus of swine (a TGEV vaccine). An inactivated vaccine composition for use in such a method and a process for the manufacture of the inactivated vaccine composition are described.

Abstract: The recombinant live vaccine comprises, as vector, a feline herpesvirus comprising and expressing at least one nucleotide sequence encoding a polypeptide, this sequence being inserted into the ORF5 and/or ORF2 sites. Polyvalent vaccine formula and feline herpesvirus DNA fragments.

Abstract: A method for disinfecting a meat carcass by spray application of an aqueous solution containing from about 0.05-0.12% of a metal chlorite and a sufficient quantity of an acid having a pK.sub.a of from about 2.0-4.4 to adjust the pH of the aqueous solution to about 2.2-4.5 and to maintain the chlorite ion concentration in the form of chlorous acid to not more than about 35% by weight of the aqueous solution, the molar ratio of the acid to metal chlorite being at least equal to the first pK.sub.a of the acid multiplied by the grams/liter concentration of metal chlorite in the aqueous solution. In one embodiment, the meat carcass is poultry.

Abstract: This invention relates to polynucleotides encoding Glycoprotein B from the RFHV/KSHV subfamily of gamma herpes viruses, three members of which are characterized in detail. DNA extracts were obtained from Macaque nemestrina and Macaque mulatta monkeys affected with retroperitoneal fibromatosis (RF), and human AIDS patients affected with Kaposi's sarcoma (KS). The extracts were amplified using consensus-degenerate oligonucleotide probes designed from known protein and DNA sequences of gamma herpes viruses. The nucleotide sequences of a 319 base pair fragment are about 76% identical between RFHV1 and KSHV, and about 60-63% identical with the closest related gamma herpes viruses outside the RFHV/KSHV subfamily. Protein sequences encoded within these fragments are are about 91% identical between RFHV1 and KSHV, and <.about.65% identical to that of other gamma herpes viruses.

Abstract: The invention relates to treatment of aqueous streams and aqueous systems with ozone. The aqueous stream comprises a closed loop system providing transport from a production locus to a processing locus for a variety of products. The aqueous stream can contain a challenge soil load and a microbial load. A treatment zone is defined through which the aqueous stream passes. The treatment zone is contacted with a sufficient concentration of ozone to substantially reduce the microbial population and to substantially reduce the number of microorganisms that can generate slime on the interior of the system. An amount of ozone is added to the aqueous stream in the treatment zone such that once the microbial populations are reduced to a safe level, the ozone concentration is also reduced to a level substantially safe for contact with operating personnel and product. Typically, such concentration is less than one part by weight of ozone per million parts by weight of aqueous system, preferably less than 0.

Abstract: Natural products such as equisetin and derivatives are described. These compounds are useful in the inhibition of HIV integrase.

Abstract: A method for producing an improved antimicrobial material thereby producing a soft, dry, iodine/acetalized polyvinyl alcohol complex sponge material having a pleasing yellow-gold coloration which self-indicates activation of the antimicrobial elements in the complex.

Abstract: What is described is a recombinant poxvirus, such as vaccinia virus, fowlpox virus and canarypox virus, containing foreign DNA from flavivirus, such as Japanese encephalitis virus, yellow fever virus and Dengue virus. In a preferred embodiment, the recombinant poxvirus generates an extracellular particle containing flavivirus E and M proteins capable of inducing neutralizing antibodies, hemagglutination-inhibiting antibodies and protective immunity against flavivirus infection. What is also described is a vaccine containing the recombinant poxvirus for inducing an immunological response in a host animal inoculated with the vaccine.

Abstract: The present invention provides a combination vaccine for use in the protection of poultry against ND comprising an expression system, such as a virus vector, expressing a NDV immunogenic protein, and a live NDV vaccine strain. It is demonstrated that such a combination vaccine affords good local and systemic protection.

Abstract: The present invention relates to the expression of the variable region of a VP2 protein from infectious bursal virus disease by recombinant baculovirus, diagnostic assays and vaccines containing the same.

Abstract: The present invention relates to the expression of the variable region of a VP2 protein from infectious bursal virus disease by recombinant baculovirus, diagnostic assays and vaccines containing the same.