Abstract: Methods for preparing cDNA libraries from single and low quantities of cells are disclosed. The methods are based on the principles of multi-strand displacement amplification or semi-random primed polymerase chain reaction. The methods typically include a step of reverse transcription and subsequent amplification of cDNA. The methods can be adapted for preparation of cDNA libraries that are representative of mRNA or whole RNA expressed by the cell or cells. The cDNA is suitable for sequencing or microarray analysis.
Abstract: A device for detecting chemical or biochemical substances in fluids for use in an electrochemical camera. The device includes a first carrier having a sensor array with a plurality of electrochemical sensors. A second carrier includes a porous layer having at least one functional region, in which specifically binding capturing molecules are immobilized. The at least one functional region is arranged directly adjacent to at least one non-functionalized region. Assigned to the at least one functional region and the at least one non-functionalized region are several sensors of the sensor array, for use as the electrochemical camera.
Type:
Grant
Filed:
March 2, 2010
Date of Patent:
June 12, 2018
Assignee:
Boehringer Ingelheim Vetmedica GmbH
Inventors:
Alexander Frey, Walter Gumbrecht, Peter Paulicka, Meinrad Schienle, Manfred Stanzel
Abstract: Lipid multilayer structures are formed by evaporating a solvent from each of a plurality of lipid solutions thereby form the lipid multilayer structures. Each lipid solution comprises the solvent and one or more lipids. Each lipid multilayer structure is a microstructure comprising one or more lipids.
Type:
Grant
Filed:
February 12, 2014
Date of Patent:
June 12, 2018
Assignee:
FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
Abstract: A fungicidal composition containing a fungicidally effective amount of a compound of Formula I-V and at least one fungicide selected from the group consisting of epoxiconazole, prothioconazole, azoxystrobin, pyraclostrobin, penthiopyrad, isopyrazam, bixafen, boscalid, prochloraz, and chlorothalonil provides synergistic control of selected fungi.
Abstract: Clinical management of human cancer is dependent on the accurate monitoring of residual and recurrent tumors. We have developed a method, called personalized analysis of rearranged ends (PARE), which can identify translocations in solid tumors. Analysis of four colorectal and two breast cancers revealed an average of nine rearranged sequences (range 4 to 15) per tumor. Polymerase chain reaction with primers spanning the breakpoints were able to detect mutant DNA molecules present at levels lower than 0.001% and readily identified mutated circulating DNA in patient plasma samples. This approach provides an exquisitely sensitive and broadly applicable approach for the development of personalized biomarkers to enhance the clinical management of cancer patients.
Type:
Grant
Filed:
July 2, 2015
Date of Patent:
May 1, 2018
Assignee:
The Johns Hopkins University
Inventors:
Bert Vogelstein, Kenneth W. Kinzler, Victor Velculescu, Luis Diaz, Rebecca J. Leary
Abstract: Disclosed herein are biomarkers related to gastric cancer. The presently identified salivary biomarkers create the basis for a gastric cancer detection bioassay with sensitivity and specificity. Means and methods for evaluating the data generated using multiple biomarkers in order to validate findings and further use of the multiplexed gastric cancer assay in clinical, diagnostic and therapeutic uses is also included.
Type:
Grant
Filed:
September 7, 2012
Date of Patent:
April 10, 2018
Assignee:
The Regents of the University of California
Abstract: Methods for improved quality control of nucleic acid containing biological samples utilized in high-throughput situations such as biorepositories engaged in the collection, processing, storage, distribution and analysis of such samples are disclosed.
Type:
Grant
Filed:
August 8, 2011
Date of Patent:
April 10, 2018
Assignee:
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
Abstract: A graphene nanomesh based charge sensor and method for producing a graphene nanomesh based charge sensor. The method includes generating multiple holes in graphene to create a graphene nanomesh with a patterned array of multiple holes; passivating an edge of each of the multiple holes of the graphene nanomesh to allow for functionalization of the graphene nanomesh; and functionalizing the passivated edge of each of the multiple holes of the graphene nanomesh with a chemical compound that facilitates chemical binding of a receptor of a target molecule to the edge of one or more of the multiple holes, wherein the receptor is a molecule that chemically binds to the target molecule, irrespective of the size of the target molecule.
Type:
Grant
Filed:
April 29, 2015
Date of Patent:
April 3, 2018
Assignees:
International Business Machines Corporation, Egypt Nanotechnology Center
Inventors:
Ali Afzali-Ardakani, Shu-jen Han, Amal Kasry, Ahmed Maarouf, Glenn J. Martyna, Razvan Nistor, Hsinyu Tsai
Abstract: Provided are methods for determining skeletal muscle mass in subject. Also provided are methods for diagnosing a neurological condition or disease or a condition or disease associated with reduced skeletal muscle mass in a subject. Further provided are purified antibodies that bind specifically to a BIN1 polypeptide that is expressed specifically in skeletal muscle.
Abstract: The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, by compartmentalizing the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsules; and identifying the compound which binds to or modulates the activity of the target. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.
Type:
Grant
Filed:
August 12, 2011
Date of Patent:
January 2, 2018
Assignee:
Medical Research Council
Inventors:
Andrew David Griffiths, Chris Abell, Florian Hollfelder, Enrico Mastrobattista
Abstract: Methods and an apparatus according to the present invention, can be used in capture/enrichment, gene expression profiling and targeted sequencing. Provided are methods for improving accuracy and stringency of microarrays and/or other genomic analysis methods relying on nucleic acid hybridization and melting curve analysis by controlling surface chemistry. This method comprises producing a positively charged surface or surface coating, on the surface of microarray slides or other types of surfaces similarly purposed, such as micro beads, which enhances melting curve analysis to the point of allowing detection or differentiation of small changes in sequences between nucleic acid binding partners. Also provided is an improved microarray reader machine, to collect melting curve data on microarray slides.
Abstract: Concentrations of certain miRNA, mRNA and/or protein markers in the biological fluids and/or tissues of a subject are used to determine the probability that the subject does or does not have Posttraumatic Stress Disorder (PTSD). The concentrations of these markers in fluids and/or tissues are different in subjects with PTSD as compared to subjects who do not suffer from this disorder.
Type:
Grant
Filed:
March 14, 2013
Date of Patent:
January 2, 2018
Assignee:
INSTITUTE FOR SYSTEMS BIOLOGY
Inventors:
Leroy Hood, Kai Wang, Inyoul Lee, Yong Zhou, Ji Hoon Cho, Dhimankrishna Ghosh
Abstract: The invention provides methods, devices, compositions and kits for diagnosing or predicting transplant status or outcome in a subject who has received a transplant.
Type:
Grant
Filed:
February 24, 2014
Date of Patent:
December 19, 2017
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Inventors:
Stephen R. Quake, Thomas M. Snyder, Hannah Valantine
Abstract: The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control, The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.
Type:
Grant
Filed:
October 12, 2005
Date of Patent:
December 12, 2017
Assignees:
National Science Foundation, Medical Research Council, President and Fellows of Harvard College
Inventors:
Andrew Griffiths, David Weitz, Darren Link, Keunho Ahn, Jerome Bibette
Abstract: Methods are disclosed herein for detecting a genetic predisposition to focal segmental glomerulosclerosis (FSGS) or hypertensive end-stage kidney disease (ESKD) or both in a human subject, e.g., by detecting the presence of at least one single nucleotide polymorphism (SNP) in an APOL1 gene, such as the C-terminal exon of an APOL1 gene. In a further embodiment, methods are disclosed for detecting resistance of a subject to a disease associated with Trypanosoma infection, e.g., by detecting at least one single nucleotide polymorphism (SNP) in an APOL1 gene, such as the C-terminal exon of an APOL1 gene. Also disclosed are methods for treating a subject infected with T. brucei. The methods include administering a therapeutically effective amount of an APOL1 protein including a S342G substitution, an I384M substitution, and/or a deletion of N388 and Y389 to the subject.
Type:
Grant
Filed:
April 18, 2011
Date of Patent:
November 28, 2017
Assignees:
Wake Forest University Health Sciences, Beth Israel Deaconess Medical Center, Inc.
Inventors:
Giulio Genovese, David J. Friedman, Martin R. Pollak, Barry I. Freedman
Abstract: The present invention provides a rapid, sensitive method for forensic drug testing in a post-mortem subject using oral fluid collected from the post-mortem subject. The method comprises collecting a sample of oral fluid from a post-mortem subject, analyzing the oral fluid sample qualitatively to detect the presence of one or more non-naturally occurring drugs, analyzing the oral fluid sample quantitatively to determine concentration of the one or more non-naturally occurring drugs in the post-mortem subject, and identifying the one or more non-naturally occurring drugs in the post-mortem subject. The detection and quantification in oral fluid is more sensitive and faster than detection and quantification of the non-naturally occurring drugs in blood, urine, bile, and liver tissue collected from the same post-mortem subject. Further, the qualitative and quantitative results are obtained in as little as three hours.
Abstract: Device for use in a biosensor comprising a multisite array of test sites, the device being useful for modulating the binding interactions between a (biomolecular) probe or detection agent and an analyte of interest from a biological by modulating the pH or ionic gradient near the electrodes in such biosensor. The device provides a biosensor which is more accurate, reliable and the results of which are more reproducible. Analytic methods for more accurately measuring an analyte of interest in a biological sample are also provided.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
November 7, 2017
Assignee:
ROBERT BOSCH GMBH
Inventors:
Nadezda Fomina, Christopher Johnson, Sam Kavusi, Habib Ahmad
Abstract: This invention provides methods and kits for diagnosing, ascertaining the clinical course of minimal residual disease associated with acute lymphoblastic leukemia (ALL). Specifically the invention provides methods and kits useful in the diagnosis and determination of clinical parameters associated with diseases associated with ALL based on patterns of surface marker expression unique to ALL.
Abstract: A graphene nanomesh based charge sensor and method for producing a graphene nanomesh based charge sensor. A graphene nanomesh based charge sensor includes a graphene nanomesh with a patterned array of multiple holes created by generating multiple holes in graphene in a periodic way, wherein: an edge of each of the multiple holes of the graphene nanomesh is passivated; and the passivated edge of each of the multiple holes of the graphene nanomesh is functionalized with a chemical compound that facilitates chemical binding of a receptor of a target molecule to the edge of one or more of the multiple holes, allowing the target molecule to bind to the receptor, causing a charge to be transferred to the graphene nanomesh to produce a graphene nanomesh based charge sensor for the target molecule.
Type:
Grant
Filed:
April 29, 2015
Date of Patent:
September 19, 2017
Assignees:
International Business Machines Corporation, Egypt Nanotechnology Center
Inventors:
Ali Afzali-Ardakani, Shu-jen Han, Amal Kasry, Ahmed Maarouf, Glenn J. Martyna, Razvan Nistor, Hsinyu Tsai