Patents Examined by Angela Bertagna
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Patent number: 8980562Abstract: The invention relates to a method of detecting HPV and determining HPV type.Type: GrantFiled: June 12, 2008Date of Patent: March 17, 2015Assignee: Physicians Reference LaboratoryInventor: Pradip Manna
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Patent number: 8043807Abstract: Methods and kits for (i) determining a risk of a subject to develop cancer; (ii) evaluating an effectiveness and dosage of cancer therapy administered to a cancer patient; and (iii) determining a presence of correlation or non-correlation between an activity of at least one DNA repair enzyme and at least one cancer, are disclosed.Type: GrantFiled: March 21, 2002Date of Patent: October 25, 2011Assignee: Yeda Research and Development Co. Ltd.Inventors: Zvi Livneh, Tamar Paz-Elizur, Sara Blumenstein
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Patent number: 8017357Abstract: The present invention provides a nucleic acid synthesis method which involves the step of incubating a double-stranded nucleic acid template under conditions that ensure a complementary strand synthesis reaction using a primer as an origin. This method involves the step of placing a region, to which a primer capable of isothermally amplifying the template nucleic acid will anneal, in a condition that ensures base pairing, using an arbitrary primer. The arbitrary primer initiates the complementary strand synthesis reaction, using the double-stranded nucleic acid as a template and DNA polymerases catalyzing the complementary strand synthesis reaction which comprises the destabilization of the double-stranded nucleic acid and strand displacement, thereby providing a region that can undergo base pairing.Type: GrantFiled: March 30, 2001Date of Patent: September 13, 2011Assignee: Eiken Kagaku Kabushiki KaishaInventors: Tsugunori Notomi, Kentaro Nagamine
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Patent number: 8017360Abstract: Methods for detecting and optionally quantitating one or more target nucleic acids are provided, in which a surrogate nucleic acid is captured to each target nucleic acid, amplified, and detected. Compositions, kit, and systems related to the methods are also described.Type: GrantFiled: November 9, 2006Date of Patent: September 13, 2011Assignee: Panomics, Inc.Inventors: Yuling Luo, Son Bui
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Patent number: 7993881Abstract: A method for detecting pathogens, particularly organisms associated with sexually transmitted diseases, especially Human papilloma virus genotypes is described. The method involves the use of real-time PCR using specially designed probes. The probes, kits for carrying out the method, and methods for designing primers suitable for use in the method of the invention are also described.Type: GrantFiled: November 15, 2006Date of Patent: August 9, 2011Assignee: Genoid KftInventors: Csaba Jeney, Tibor Takacs
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Patent number: 7993840Abstract: The present invention relates to a method and device for detecting and/or quantifying one or multiple target molecules present in a solution by quantifying online their binding on specific capture molecules immobilized at different locations (spots) of a surface of an optically transparent solid support without substantial detection of target molecules present in solution. The present invention allows multiple target assays to be performed in a simultaneous detection. More particularly, the invention comprises detecting in real-time the hybridization between capture DNA molecules present on a micro-array and target polynucleotides present in solution. The invention is also related to real-time PCR of multiple targets on a micro-array.Type: GrantFiled: January 21, 2009Date of Patent: August 9, 2011Assignee: Eppendorf AGInventors: Isabelle Alexandre, Heinz Koehn, Jose Remacle, Sven De Roeck
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Patent number: 7989164Abstract: Method and apparatus which uses harmonic cantilevers, such as used in atomic force microscopy, to detect variations in the attractive and repulsive forces on a solid surface as a result of macromolecular binding, for example, hybridization of a single stranded DNA molecule attached to the surface with another DNA molecule. The complexed macromolecule is less flexible than an uncomplexed molecule. It will typically have more negative charge due to amino acids or DNA monomers. Both stiffness of the surface and the attractive capillary forces will change after binding and may be detected. By scanning the harmonic cantilever across a surface with macromolecules attached in tapping-mode and by recording the signals at the high frequency vibrations provided by harmonic cantilever, complexed molecules on a surface may be identified and quantified.Type: GrantFiled: April 13, 2006Date of Patent: August 2, 2011Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Ozgur Sahin, Calvin F. Quate, Olav Solgaard
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Patent number: 7985553Abstract: The invention provides methods and compositions for isolation and assessment of riboprotein complexes.Type: GrantFiled: October 29, 2002Date of Patent: July 26, 2011Inventors: Nathaniel Heintz, Tito A. Serafini, Andrew W. Shyjan
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Patent number: 7781162Abstract: The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples.Type: GrantFiled: September 11, 2003Date of Patent: August 24, 2010Assignee: Ibis Biosciences, Inc.Inventors: David J. Ecker, Richard H. Griffey, Rangarajan Sampath, Steven A. Hofstadler, John McNeil, Stanley T. Crooke
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Patent number: 7632642Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.Type: GrantFiled: October 17, 2005Date of Patent: December 15, 2009Assignee: Brandeis UniversityInventors: Lawrence J. Wangh, John Rice, J. Aquiles Sanchez, Kenneth Pierce, Jesse Salk, Arthur Reis, Cristina Hartshorn
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Patent number: 7618773Abstract: A method for the selective amplification of a target nucleic acid in a sample comprising the target nucleic acid and at least one non-target nucleic acid, the method comprising amplifying the nucleic acids by means of at least one oligonucleotide primer comprising: a primer region that can prime and extend on the target and non-target nucleic acids; and a region that is an inverted repeat of an internal sequence of an amplicon of the at least one non-target nucleic acid but which contains at least one mismatch to the corresponding internal sequence, if present, of an amplicon of the target nucleic acid.Type: GrantFiled: February 26, 2003Date of Patent: November 17, 2009Assignee: Commonwealth Scientific and Industrial Research OrganisationInventors: Keith Rand, Peter Laurence Molloy
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Patent number: 7615621Abstract: Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.Type: GrantFiled: October 3, 2006Date of Patent: November 10, 2009Assignee: Monsanto Technology LLCInventors: Mark A. Dizigan, Rebecca A. Kelly, Dale A. Voyles, Michael Hans Luethy, Thomas M. Malvar, Kathleen P. Malloy
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Patent number: 7601499Abstract: The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.Type: GrantFiled: June 6, 2006Date of Patent: October 13, 2009Assignee: 454 Life Sciences CorporationInventors: Jan Berka, Zhoutao Chen, Michael Egholm, Brian C. Godwin, Stephen Kyle Hutchison, John Harris Leamon, Gary James Sarkis, Jan Fredrik Simons
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Patent number: 7579151Abstract: Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.Type: GrantFiled: July 27, 2005Date of Patent: August 25, 2009Assignee: Samsung Electronics Co., Ltd.Inventors: Young-sun Lee, Jung-im Han, Hyo-yeon Lee, Kak Namkoong, Kwang-wook Oh
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Patent number: 7563574Abstract: Methods of monitoring enzyme mediated reactions, and particularly nucleic acid synthesis reactions such as pyrosequencing methods that employ enzymatic reporter systems. The methods and systems provide elevated signal levels as compared to conventional pyrosequencing processes, and/or mediate de-phasing of sequencing analyses employing pyrosequencing or other “sequencing by synthesis” methods.Type: GrantFiled: March 31, 2006Date of Patent: July 21, 2009Assignee: Pacific Biosciences of California, Inc.Inventors: Jonas Korlach, Stephen Turner
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Patent number: 7553626Abstract: Provided are a primer set for amplifying a target sequence specific to Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Stapylococcus aureus, and Streptococcus pneumoniae, the primer set including at least one oligonucleotide of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 1 and at least one oligonucleotides of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 2; and probes specific to a specific species of the 10 bacterial species.Type: GrantFiled: December 23, 2005Date of Patent: June 30, 2009Assignee: Samsung Electronics Co., Ltd.Inventors: Ji-young Oh, Nam Huh, Sang-hyun Peak, Sung-young Jeong
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Patent number: 7547510Abstract: The present invention relates to thermostable proteinases from thermophilic Bacillus species and their uses in the preparation of nucleic acid samples. The enzymes of the invention are stable and active at 65-80° C., but are readily autolysed or denatured above 90° C.Type: GrantFiled: May 14, 2002Date of Patent: June 16, 2009Assignee: Zygem Corporation LimitedInventors: Roy McIver Daniel, David James Saul
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Patent number: 7465562Abstract: Biological samples containing nucleic acids, RNA and DNA, are freed from bound proteins by incubation with a chaotropic agent such as a guanidium salt, and the mixture is readied for further processing by dilution of such agent to a concentration below 0.05 M without physical isolation of RNA and DNA from one another or from other components of the reaction mixture. Methods include such preparation and further processing, such as amplification and detection, which may be performed in a single container. Chaotropic agent may be supplied as a dried reagent adhered to a container. Kits may include such reagents, alone or with amplification reagents.Type: GrantFiled: May 18, 2004Date of Patent: December 16, 2008Assignee: Brandeis UniversityInventors: Lawrence J. Wangh, Cristina Hartshorn
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Patent number: 7462452Abstract: The present invention provides novel compositions, methods and apparatus for DNA sequencing that can be performed, e.g., in a two-electrode chamber. The present invention also provides a method for sequencing a nucleic acid comprising immobilizing a plurality of complexes comprising a target nucleic acid, a primer nucleic acid, and a polymerase onto a surface, contacting the surface with a plurality of charged particles comprising a nucleotide phosphate by applying an electric field, reversing the electric field to transport unbound charged particles away from the surface, and detecting the incorporation of a nucleotide phosphate into a single molecule of the primer nucleic acid.Type: GrantFiled: April 29, 2005Date of Patent: December 9, 2008Assignee: Pacific Biosciences of California, Inc.Inventors: John G. K. Williams, Jon P. Anderson
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Patent number: 7455971Abstract: The invention relates to compositions and methods for multiplex decoding of microsphere array sensors.Type: GrantFiled: November 22, 2005Date of Patent: November 25, 2008Assignee: Illumina, Inc.Inventors: Mark S. Chee, John R. Stuelpnagel, Anthony W. Czarnik