Patents Examined by Anoop K. Singh
  • Adeno-associated virus vector delivery of micro-dystrophin to treat muscular dystrophy
    Patent number: 11723986
    Abstract: The invention provides for recombinant AAV vectors comprising a miniaturized human micro-dystrophin gene and methods of using the recombinant vectors to reduce or prevent fibrosis in subjects suffering from muscular dystrophy.
    Type: Grant
    Filed: April 14, 2017
    Date of Patent: August 15, 2023
    Assignee: RESEARCH INSTITUTE AT NATIONWIDE CHILDREN'S HOSPITAL
    Inventors: Louise Rodino-Klapac, Jerry R. Mendell
  • Maintenance culture of induced pluripotent stem cell-derived intestinal stem cells
    Patent number: 11725189
    Abstract: It is an object to provide a culture method which is capable of maintaining and/or culturing iPS cell-derived intestinal stem cells, while maintaining the properties of intestinal stem cells. The induced pluripotent stem cell-derived intestinal stem cell-like cells are cultured in the presence of a GSK-3? inhibitor, a histone deacetylation inhibitor, and a serum replacement, or in the presence of a GSK-3? inhibitor and a serum replacement. Preferably, the culture is carried out under conditions in which one or more compounds selected from the group consisting of an epidermal growth factor, a TGF? receptor inhibitor and a fibroblast growth factor are further present.
    Type: Grant
    Filed: August 20, 2019
    Date of Patent: August 15, 2023
    Assignees: PUBLIC UNIVERSITY CORPORATION NAGOYA CITY UNIVERSITY, FUJIFILM Corporation
    Inventors: Takahiro Iwao, Tamihide Matsunaga, Satoshi Kondo, Shota Mizuno
  • Human liver chimeric mouse with deficient P450 oxidoreductase
    Patent number: 11716974
    Abstract: The present disclosure provides a chimeric non-human animal comprising human hepatocytes, methods for preparing the chimeric non-human animal comprising human hepatocytes and methods of utilizing the chimeric non-human animal comprising human hepatocytes to screening and identifying metabolites for any type of drugs, typically small molecule drugs, which might affect human liver functions and any other bodily function.
    Type: Grant
    Filed: June 27, 2017
    Date of Patent: August 8, 2023
    Assignee: Baylor College of Medicine
    Inventors: Karl-Dimiter Bissig, Maria de las Mercedes Barzi Dieguez, Peter Francis Pankowicz
  • Method and facility for breeding insects
    Patent number: 11712027
    Abstract: The present invention relates to a method and facility for breeding insects. According to the invention, the insect breeding facility comprises a spawning area comprising spawning containers which are adapted to receive adult insects and insect food, wherein at least one spawn structure is provided in each spawning container, in which spawn structures the mother insects will spawn their eggs. Furthermore a hatch area is provided in which the eggs will hatch and which allows periodical harvesting of baby larvae from the hatch area. The hatch area comprises a plurality of hatching chambers corresponding in number with the number of batches of spawning containers.
    Type: Grant
    Filed: March 10, 2016
    Date of Patent: August 1, 2023
    Assignee: YNSECT NL R&D B.V.
    Inventors: Hans Calis, Pieter Johannes Antonius Franken
  • Differentiation of human embryonic stem cells
    Patent number: 11708562
    Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides an improved method for the formation of pancreatic endoderm, pancreatic hormone expressing cells and pancreatic hormone secreting cells. The present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer.
    Type: Grant
    Filed: October 10, 2019
    Date of Patent: July 25, 2023
    Assignee: Janssen Biotech, Inc.
    Inventors: Alireza Rezania, Jean Xu
  • In vitro methods of differentiating stem cells into neurons and neurons generated using the same
    Patent number: 11702630
    Abstract: Methods of generating spinal cord glutamatergic interneurons (V2a interneurons) from human pluripotent stem cells (hPSCs) are provided. A method of the present disclosure may include culturing a first population of hPSCs in vitro in a neural induction medium that includes: a retinoic acid signaling pathway activator; a sonic hedgehog (Shh) signaling pathway activator; and a Notch signaling pathway inhibitor, wherein the culturing results in generation of a second population of cultured cells containing CHX10+ V2a interneurons. Also provided are non-human animal models that include the hPSC-derived spinal cord glutamatergic interneurons, and methods of producing the non-human animal models.
    Type: Grant
    Filed: May 26, 2017
    Date of Patent: July 18, 2023
    Assignees: The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone, The Regents of the University of California, Georgia Tech Research Corporation
    Inventors: Jessica Butts, Todd C. McDevitt
  • Acceleration of stem cell differentiation
    Patent number: 11702629
    Abstract: Provide herein are compositions, methods and kits to accelerate pluripotent stem cell differentiation.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: July 18, 2023
    Assignee: Regents of the University of Minnesota
    Inventors: Patrick Joseph Walsh, James Robert Dutton, Ann Margaret Parr
  • Importation of mitochondrial protein by an enhanced allotopic approach
    Patent number: 11702671
    Abstract: An expression vector containing appropriate mitochondrion-targeting sequences (MTS) and appropriate 3?UTR sequences provides efficient and stable delivery of a mRNA encoding a protein (CDS) to the mitochondrion of a mammalian cell. The MTS and 3?UTR sequences guide the CDS mRNA from the nuclear compartment of the cell to mitochondrion-bound polysomes, where the CDS is translated. This provides an efficient translocation of a mature functional protein into the mitochondria. A method of targeting mRNA expressed in the nuclear compartment of a mammalian cell to the mitochondrion is also provided. The vector and methods can be used to treat defects in mitochondrial function.
    Type: Grant
    Filed: May 26, 2021
    Date of Patent: July 18, 2023
    Assignee: INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)
    Inventors: Marisol Corral-Debrinski, Jose-Alain Sahel, Valerie Kaltimbacher, Crystel Bonnet
  • AAV-mediated delivery of therapeutic antibodies to the inner ear
    Patent number: 11697801
    Abstract: Provided herein are methods that include introducing into an inner ear of a mammal a therapeutically effective amount of an adeno-associated virus (AAV) vector that includes a nucleotide sequence encoding (a) a polypeptide including an antibody heavy chain variable domain operably linked to a signal peptide and a polypeptide including an antibody light chain variable domain operably linked to a signal peptide; (b) a polypeptide including an antigen-binding antibody fragment operably linked to a signal peptide; or (c) a soluble vascular endothelial growth factor receptor operably linked to a signal peptide.
    Type: Grant
    Filed: August 3, 2021
    Date of Patent: July 11, 2023
    Assignee: Akouos, Inc.
    Inventors: Emmanuel John Simons, Robert Ng, Michael McKenna
  • Media and methods for enhancing the survival and proliferation of stem cells
    Patent number: 11697796
    Abstract: The present disclosure relates to improved supplements, culture media and methods for enhancing the survival or proliferation of mammalian stem cells. In particular, adding a lipid supplement, such as a lipid-enriched carrier (e.g. a lipid-enriched albumin), to the culture medium may enhance the survival and/or proliferation of the stem cells by at least 5% to 65% as compared to a culture medium that does not contain the lipid supplement.
    Type: Grant
    Filed: January 23, 2018
    Date of Patent: July 11, 2023
    Assignee: STEMCELL TECHNOLOGIES CANADA INC.
    Inventors: Adam Hirst, Arwen Hunter, Melanie Kardel, Wing Chang
  • Methods for expanding and activating ?? T cells for the treatment of cancer and related malignancies
    Patent number: 11690872
    Abstract: The present disclosure relates to expansion and activation of T cells. In an aspect, the present disclosure relates to expansion and activation of ?? T cells that may be used for transgene expression. In another aspect, the disclosure relates to expansion and activation of ?? T cells while depleting ?- and/or ?-TCR positive cells. T cell populations comprising expanded ?? T cell and depleted or reduced ?- and/or ?-TCR positive cells are also provided for by the instant disclosure. The disclosure further provides for methods of using the disclosed T cell populations.
    Type: Grant
    Filed: November 26, 2018
    Date of Patent: July 4, 2023
    Assignee: IMMATICS US, INC.
    Inventors: Monique Dao, Steffen Walter, Melinda Mata, Aleksandra Nowicka, Yannick Bulliard, Sarah Missell, Sabrina Kuttruff-Coqui, Norbert Hilf
  • Compositions and methods for organoid generation and disease modeling
    Patent number: 11685901
    Abstract: The invention features pancreatic islet and pancreatic organoids, and cell cultures and methods that are useful for the rapid and reliable generation of pancreatic islet and pancreatic islet organoids. The invention also features methods of treating pancreatic diseases and methods of identifying agents that are useful for treatment of pancreatic diseases, such as type 2 diabetes and pancreatic cancer, using the pancreatic islet and pancreatic organoids of the invention.
    Type: Grant
    Filed: March 28, 2022
    Date of Patent: June 27, 2023
    Assignee: Salk Institute for Biological Studies
    Inventors: Ronald Evans, Michael Downes, Annette Atkins, Eiji Yoshihara, Ruth Yu
  • Methods of making human primitive ectoderm cells
    Patent number: 11667889
    Abstract: This disclosure relates to compositions comprising human preprimitive streak cells and/or human mesendoderm cells as well as methods for their production. Additionally, disclosed herein are methods of identifying factors useful in the further differentiation of preprimitive streak and mesendoderm cell types.
    Type: Grant
    Filed: December 17, 2019
    Date of Patent: June 6, 2023
    Assignee: ViaCyte, Inc.
    Inventors: Kevin Allen D'Amour, Alan D. Agulnick, Susan Eliazer, Evert Kroon, Emmanuel E. Baetge
  • Barrier function measurements
    Patent number: 11662342
    Abstract: The current invention relates to an improved method for measuring modulating effects of compounds on epithelial cell barrier function. The methods allows for high throughput screening of test compounds individually or in combination. Compounds improving the epithelial barrier function or compounds negatively influencing epithelial barrier function can be analyzed with the method described herein.
    Type: Grant
    Filed: July 8, 2016
    Date of Patent: May 30, 2023
    Assignee: MIMETAS B.V.
    Inventors: Paul Vulto, Sebastiaan Johannes Trietsch, Henriëtte Leonore Lanz, Marianne Katharina Vormann
  • Placenta-derived matrix and methods of preparing and use thereof
    Patent number: 11655448
    Abstract: The invention relates to placenta-derived matrix, methods of preparing, and methods of use thereof. The invention also relates to methods of culturing cells, delivering cells, promoting differentiation of stem cells or tissue-specific progenitor cells, and repairing, replacing, regenerating, filling, reducing or inhibiting scarring of defects using the same. The invention further relates to methods of coating placenta-derived matrix on a surface or injecting the placenta-derived matrix into a site of interest.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: May 23, 2023
    Assignee: LifeNet Health
    Inventors: Michael Francis, Silvia Chen, Erick Breathwaite, Rudy Rodriguez, Alan Smith, Alexander Huber, Jung Bok Lee
  • Simultaneous spatio-temporal measurement of gene expression and cellular activity
    Patent number: 11624086
    Abstract: Provided herein are methods for simultaneous spatio-temporal measurement of gene expression and cellular activity.
    Type: Grant
    Filed: December 3, 2021
    Date of Patent: April 11, 2023
    Assignee: 10x Genomics, Inc.
    Inventors: Cedric Uytingco, Layla Katiraee, Kristen Pham
  • Method for treating Huntingtons's disease
    Patent number: 11612641
    Abstract: The present disclosure provides methods for treating or preventing Huntington's disease (HD) in a subject in need thereof, comprising administering a therapeutic agent that activates mTORC 1 function and/or increases Ras Homolog Enriched in Striatum (Rhes) level in the subject's brain as compared to the function or level in the subject prior to treatment, and methods for modulating mHTT-associated metabolic phenotypes and/or reversal of striatal atrophy by administering a therapeutic agent that activates mTORC1 function and/or increases Ras Homolog Enriched in Striatum (Rhes) level in the subject's brain as compared to the function or level in the subject prior to treatment.
    Type: Grant
    Filed: December 30, 2015
    Date of Patent: March 28, 2023
    Assignee: UNIVERSITY OF IOWA RESEARCH FOUNDATION
    Inventors: Beverly L. Davidson, John H. Lee
  • Nucleic acid construct
    Patent number: 11613559
    Abstract: The present invention provides a nucleic acid construct comprising the following structure: A-X-B in which A is nucleic acid sequence encoding a first polypeptide which comprises a first signal peptide; B is nucleic acid sequence encoding a second polypeptide which comprises a second signal peptide and X is a nucleic acid sequence which encodes a cleavage site, wherein the first signal peptide or the second signal peptide comprises one or more mutation(s) such that it has fewer hydrophobic amino acids.
    Type: Grant
    Filed: April 26, 2016
    Date of Patent: March 28, 2023
    Assignee: AUTOLUS LIMITED
    Inventors: Martin Pulé, Shaun Cordoba
  • Method for establishing modified host cell
    Patent number: 11608508
    Abstract: An object of the present invention is to establish a cell line that is useful as a host cell for use in recombinant protein production, highly expresses transgenes stably, and grows stably. The present invention provides a method for establishing a cell line for recombinant protein production capable of stably expressing two or more foreign genes, comprising transferring a gene of a non-coding RNA suppressing the expression of NfkBia to a cell.
    Type: Grant
    Filed: October 10, 2013
    Date of Patent: March 21, 2023
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventor: Hisahiro Tabuchi
  • Methods for modifying genomic DNA
    Patent number: 11608511
    Abstract: Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: contacting the cells with an activating composition; transfecting the cells with a transfection composition comprising (a) donor DNA and (b) a DNA digesting agent; wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region; and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.
    Type: Grant
    Filed: April 13, 2016
    Date of Patent: March 21, 2023
    Assignee: MaxCyte, Inc.
    Inventors: Linhong Li, Madhusudan Peshwa