Patents Examined by Arthur S Leonard
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Patent number: 11976096Abstract: The present disclosure provides recombinant AAV capsid proteins comprising a modification in the amino acid sequence and virus vectors comprising the recombinant AAV capsid proteins. The disclosure also provides methods of administering the virus vectors and vims capsids of the disclosure to a cell or to a subject in vivo.Type: GrantFiled: September 26, 2023Date of Patent: May 7, 2024Assignee: Ginkgo Bioworks, Inc.Inventors: Daniel McCoy, Garrett E. Berry
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Patent number: 11975033Abstract: Provided herein are recombinant microorganisms that express a subject polypeptide. Microorganisms can comprise an expression construct comprising a flagellin promoter operatively linked with a heterologous nucleotide sequence encoding the subject polypeptide. The flagellin promoter sequence can comprise a genetic modification that reduces CsrA inhibition of translation. Microorganisms also can comprise a genetic modification that reduces FlgM inhibition of SigD initiation of transcription. The target polypeptide can be an aldehyde dehydrogenase. Such microorganisms are useful in the treatment of alcohol hangover.Type: GrantFiled: October 26, 2020Date of Patent: May 7, 2024Assignee: ZBiotics CompanyInventor: Zachary Abbott
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Patent number: 11974553Abstract: Described herein are compositions (e.g. cells and transgenic animals) and methods relating to engineered Ig loci that permit expression of particular antibodies or antibody segments while still permitting recombination and/or maturation process for antibody optimization.Type: GrantFiled: June 25, 2018Date of Patent: May 7, 2024Assignee: CHILDREN'S MEDICAL CENTER CORPORATIONInventors: Frederick W. Alt, Hwei-Ling Cheng, Ming Tian
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Patent number: 11959079Abstract: The present disclosure provides a method of increasing the functionality of a GABAergic interneuron in the hilus of the hippocampus of an individual having at least one apolipoprotein E4 (apoE4) allele. The method generally involves reducing tau levels in the interneuron.Type: GrantFiled: December 22, 2017Date of Patent: April 16, 2024Assignee: The J. David Gladstone Institutes, a testamentary trust established under the of Will of J. David GladstoneInventors: Yadong Huang, Yaisa Andrews-Zwilling
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Patent number: 11952587Abstract: Healthy functional platelets are mass produced. A method for producing platelets, comprising: (1) a culture step of culturing megakaryocytes in a platelet producing medium in the presence of mechanical stress and platelet production promoting factors including MIF, NRDc, IGFBP2, TSP-1, PAI-1, and CCL5, and (2) a harvest step of harvesting the platelets obtained by the culture step; wherein the culture step comprises: (a) a step of promoting a release of the platelet production promoting factors from megakaryocytes by mechanical stress; and/or (b) a step of externally adding platelet production promoting factors including MIF, NRDc, and IGFBP2.Type: GrantFiled: March 5, 2018Date of Patent: April 9, 2024Assignees: KYOTO UNIVERSITY, MEGAKARYON CORPORATIONInventors: Koji Eto, Sou Nakamura, Yukitaka Ito
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Patent number: 11946048Abstract: A non-human papilloma pseudovirus or virus like particle has at least one papilloma capsid protein codon-optimized for expression in eukaryotic cells or cell lines. A pharmaceutical composition includes the non-human papilloma pseudovirus or virus like particle, and a diagnostic agent, an imaging agent, and a therapeutic agent. A non-human papilloma pseudovirus or virus like particle can be used as a medicament. A method for producing a non-human papilloma pseudovirus or virus like particle involves codon-optimizing of capsid proteins of non-human papillomaviruses for expression in eukaryotic cells, synthesizing of the sequences and cloning of the synthesized sequences into expression vectors, and producing non-human papilloma pseudovirus or virus like particles. ?-carrageenan can be used as transduction enhancer for non-human papilloma pseudovirus or virus like particles in vitro.Type: GrantFiled: November 13, 2018Date of Patent: April 2, 2024Assignees: FRAUNHOFER-GESELLSCHAFT ZUR FĂ–RDERUNG DER ANGEWANDTEN FORSCHUNG E.V., ANGEWANDTEN FORSCHUNG E.V.Inventors: Thomas Grunwald, Lea Bayer
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Patent number: 11932870Abstract: Compounds that either produced a higher proportion or greater absolute number of phenotypically identified naive, stem cell memory, central memory T cells, adaptive NK cells, and type I NKT cells are identified. Compositions and methods for modulating immune cells including T, NK, and NKT cells for adoptive cell therapies with improved efficacy are provided.Type: GrantFiled: December 4, 2017Date of Patent: March 19, 2024Inventors: Jonathan Rosen, Betsy Denise Rezner, Ian Hardy, Eigen Peralta
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Patent number: 11925664Abstract: Genetically modified compositions, such as non-viral vectors and T cells, for treating cancer are disclosed. Also disclosed are the methods of making and using the genetically modified compositions in treating cancer.Type: GrantFiled: June 12, 2020Date of Patent: March 12, 2024Assignees: INTIMA BIOSCIENCE, INC., REGENTS OF THE UNIVERSITY OF MINNESOTA, THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICESInventors: Branden Moriarity, Beau Webber, David Largaespada, Modassir Choudhry, Steven A. Rosenberg
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Patent number: 11926845Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.Type: GrantFiled: January 29, 2021Date of Patent: March 12, 2024Assignee: Hitachi, Ltd.Inventors: Yuki Niimi, Hiroko Hanzawa, Maiko Tanabe, Kunio Ohyama, Shizu Takeda
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Patent number: 11912986Abstract: Understanding the complex effects of genetic perturbations on cellular state and fitness in human pluripotent stem cells (hPSCs) has been challenging using traditional pooled screening techniques which typically rely on unidimensional phenotypic readouts. Here, Applicants use barcoded open reading frame (ORF) overexpression libraries with a coupled single-cell RNA sequencing (scRNA-seq) and fitness screening approach, a technique we call SEUSS (ScalablE fUnctional Screening by Sequencing), to establish a comprehensive assaying platform. Using this system, Applicants perturbed hPSCs with a library of developmentally critical transcription factors (TFs), and assayed the impact of TF overexpression on fitness and transcriptomic cell state across multiple media conditions. Applicants further leveraged the versatility of the ORF library approach to systematically assay mutant gene libraries and also whole gene families.Type: GrantFiled: September 22, 2020Date of Patent: February 27, 2024Assignee: The Regents of the University of CaliforniaInventors: Prashant Mali, Udit Parekh, Yan Wu, Kun Zhang
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Patent number: 11904023Abstract: The present invention relates in part to nucleic acids, including nucleic acids encoding proteins, therapeutics and cosmetics comprising nucleic acids, methods for delivering nucleic acids to cells, tissues, organs, and patients, methods for inducing cells to express proteins using nucleic acids, methods, kits and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, therapeutics, and cosmetics produced using these methods, kits, and devices.Type: GrantFiled: September 11, 2020Date of Patent: February 20, 2024Assignee: Factor Bioscience Inc.Inventors: Matthew Angel, Christopher Rohde
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Patent number: 11903966Abstract: Genetically modified compositions, such as non-viral vectors and T cells, for treating cancer are disclosed. Also disclosed are the methods of making and using the genetically modified compositions in treating cancer.Type: GrantFiled: November 5, 2018Date of Patent: February 20, 2024Assignees: REGENTS OF THE UNIVERSITY OF MINNESOTA, INTIMA BIOSCIENCE, INC., The U.S.A., as represented by the Secretary, Department f Health and Human ServicesInventors: Branden Moriarity, Beau Webber, Modassir Choudhry, Steven A. Rosenberg, Douglas C. Palmer, Nicholas P. Restifo
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Patent number: 11895992Abstract: Methods of producing non-human animal models of corneal ectatic diseases, such as corneal keratoconus, by applying an aromatic compound to the eye of a non-human animal are described. Also described are non-human animal models of corneal ectatic diseases, and methods of using the non-human animal models to screen compounds that modulate corneal ectatic diseases.Type: GrantFiled: August 12, 2019Date of Patent: February 13, 2024Inventor: Arturo Solis Herrera
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Patent number: 11883534Abstract: RNA encoding immunogen is delivered in a liposome for the purposes of immunisation. The liposome includes lipids which have a pKa in the range of 5.0 to 7.6 and, preferably, a tertiary amine. These liposomes can have essentially a neutral surface charge at physiological pH and are effective for immunisation.Type: GrantFiled: August 8, 2023Date of Patent: January 30, 2024Assignee: GLAXOSMITHKLINE BIOLOGICALS SAInventor: Andrew Geall
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Patent number: 11879133Abstract: Compositions and methods are provided for treating ocular neuropathy in a subject. In one aspect, a recombinant adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding NRF2. In another aspect, a recombinant adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding SIRT1. In desired embodiments, the subject is human, cat, dog, sheep, or non-human primate.Type: GrantFiled: April 24, 2018Date of Patent: January 23, 2024Assignee: The Trustees of the University of PennsylvaniaInventors: Jean Bennett, Junwei Sun, Kenneth Shindler, Devin McDougald, Ahmara Gibbons Ross
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Patent number: 11866729Abstract: The present invention discloses an in vitro method for the generation of a cell composition comprising or consisting of ventral midbrain dopaminergic progenitor cells from a cell composition comprising pluripotent and/or multipotent stem cells, the method comprising the steps of A) differentiating said pluripotent and/or multipotent stem cells into ventral dopaminergic progenitor cells, thereby generating a cell composition comprising ventral dopaminergic progenitor cells comprising ventral midbrain dopaminergic progenitor cells and ventral hindbrain dopaminergic progenitor cells, and B) Enriching CD117 positive cells from said cell composition comprising ventral dopaminergic progenitor cells by using an antigen binding molecule specific for the CD117 antigen, thereby generating said cell composition comprising or consisting of ventral midbrain dopaminergic progenitor cells. Cell compositions obtainable by said method are also disclosed.Type: GrantFiled: September 3, 2020Date of Patent: January 9, 2024Assignee: MILTENYI BIOTEC B.V. & CO. KGInventors: Andreas Bosio, Andrej Smiyakin
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Patent number: 11857681Abstract: RNA encoding an immunogen is delivered in a liposome for the purposes of immunisation. The liposome includes lipids which have a pKa in the range of 5.0 to 7.6 and, preferably, a tertiary amine. These liposomes can have essentially a neutral surface charge at physiological pH and are effective for immunisation.Type: GrantFiled: August 8, 2023Date of Patent: January 2, 2024Assignee: GLAXOSMITHKLINE BIOLOGICALS SAInventor: Andrew Geall
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Patent number: 11859162Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.Type: GrantFiled: August 30, 2019Date of Patent: January 2, 2024Assignee: The Charles Stark Draper Laboratory, Inc.Inventors: Vishal Tandon, Charles A. Lissandrello, Jenna L. Balestrini, Jonathan R. Coppeta, Patricia A. Swierk
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Patent number: 11851671Abstract: Compositions providing, and methods for providing and using, targeted rAAV are disclosed.Type: GrantFiled: October 23, 2018Date of Patent: December 26, 2023Assignee: Regents of the University of MinnesotaInventors: Daniel Schmidt, Wendy Gordon, Alina Catherine Zdechlik
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Patent number: 11850305Abstract: RNA encoding an immunogen is delivered in a liposome for the purposes of immunisation. The liposome includes lipids which have a pKa in the range of 5.0 to 7.6 and, preferably, a tertiary amine. These liposomes can have essentially a neutral surface charge at physiological pH and are effective for immunisation.Type: GrantFiled: August 8, 2023Date of Patent: December 26, 2023Assignee: GLAXOSMITHKLINE BIOLOGICALS SAInventor: Andrew Geall