Abstract: An isolated hTFIIIA gene having a nucleotide sequence coding for the amino acid sequence of SEQ ID NO:1, as disclosed, and in particular the hTFIIIA gene having the nucleotide sequence of SEQ ID NO:2. The gene can be used to express a corresponding hTFIIIA protein. The gene and protein serve as transcription regulating factors, and are useful in the diagnosis or identification of hereditary disease, such as cancer or other diseases resulting from abnormal transcriptional control and, further, in analyzing the mechanisms of action thereof.
Abstract: Methods are disclosed for the production and purification of hydrophobic fusion proteins production and purification of said hydrophobic polypeptides, proteins or peptides. Homogeneous monomeric .beta.-amyloid peptide and tests for screening amyloid toxicity-inhibiting drugs using this monomeric .beta.-amyloid peptide relate to these fusion proteins.
Type:
Grant
Filed:
June 29, 1994
Date of Patent:
May 12, 1998
Assignee:
Hoffmann-La Roche Inc.
Inventors:
Heinz Dobeli, Nicholas Draeger, Gerda Huber Trottman, Peter Jakob, Dietrich Stuber
Abstract: DNA encoding triol polyketide synthase (TPKS) has been isolated, purified and sequenced. Expression vectors comprising TPKS, cells transformed with the expression vectors, and processes employing the transformed cells are provided.
Type:
Grant
Filed:
May 25, 1995
Date of Patent:
April 28, 1998
Assignee:
Merck & Co., Inc.
Inventors:
Victor A. Vinci, Michael J. Conder, Phyllis C. McAda, Christopher D. Reeves, John Rambosek, Charles Ray Davis, Lee E. Hendrickson
Abstract: The present invention relates generally to identification of proteins, designated TIH proteins, that interact with casein kinase I isoforms and to isolation of polynucleotides encoding the same.
Abstract: A method for producing the derivatives of the invention by preparing a DNA fragment comprising at least the part of the coding sequence of staphylokinase that provides for its biological activity; performing in vitro site-directed mutagenesis on the DNA fragment to replace one or more codons for wild-type amino acids by a codon for another amino acid; cloning the mutated DNA fragment in a suitable vector; transforming or transfecting a suitable host cell with the vector; and culturing the host cell under conditions suitable for expressing the DNA fragment. Preferably the DNA fragment is a 466 bp EcoRI-HINDIII fragment of the plasmid pMEX602SAK, the in vitro site-directed mutagenesis is performed by an oligonucleotide-directed mutagenesis system using the plasmid pMa/c and the repair deficient E. coli strain WK6MutS, and the mutated DNA fragment is cloned in E. coli strain-WK6.
Type:
Grant
Filed:
January 11, 1995
Date of Patent:
December 9, 1997
Assignees:
Leuven Research & Development VZW, Desire Collen
Abstract: A protein and gene encoding it are disclosed which confer sensitivity to B. maydis T toxin and the insecticide methomyl, in cells carrying the gene and expressing the protein. Toxin sensitivity domains of the protein have been identified wherein a modification yields a toxin-insensitive product.
Type:
Grant
Filed:
April 21, 1995
Date of Patent:
January 28, 1997
Assignee:
Mycogen Plant Science, Inc.
Inventors:
Charles S. Levings, III, Ralph E. Dewey, Carl J. Braun
Abstract: A protein and gene encoding it are disclosed which confer sensitivity to B. maydis T toxin and the insecticide methomyl, in cells carrying the gene and expressing the protein. Toxin sensitivity domains of the protein have been identified wherein a modification yields a toxin-insensitive product.
Type:
Grant
Filed:
April 21, 1995
Date of Patent:
November 19, 1996
Assignees:
Mycogen Plant Sciences, Inc., North Carolina State University
Inventors:
Charles S. Levings, III, Ralph E. Dewey, Carl J. Braun