Abstract: This disclosure provides, among other things, a 5? adapter of the formula 3?*—X—(5?5?)—Y—3?, where: 3?* is a blocked 3? end, X is a synthetic sequence, (5?5?) is an internal 5?-5? linkage, Y is an adapter sequence, and 3? is a hydroxylated 3? end. In use, sequence X hybridizes to sequence X? in a population of RNA molecules of formula R—X?, which increases the efficiency of ligation of the 5? adapter to the nucleic acid molecules.
Abstract: The present invention relates to a nucleic acid complex pair for detecting a target nucleic acid in a sample, and more particularly to a nucleic acid complex pair used for detecting a target DNA in a sample, wherein the nucleic acid complex pair includes a first nucleic acid complex including a first determination region, a first pairing region, and a first detection region; and a second nucleic acid complex including a second determination region, a second paring region, and a second detection region, wherein the first determination region includes at least a partial domain that complementarily binds to a first target nucleic acid sequence, and the second determination region includes at least a partial domain that complementarily binds to a second target nucleic acid sequence, wherein the first pairing region and the second pairing region a domain where they can complementarily hybridize to each other.
Abstract: Methods of, and compositions for, analysis using digital amplification assays with unconventional/inverse changes in photoluminescence to indicate the presence of one or more targets. In an exemplary method, isolated volumes may be formed, only a subset of which contain a target. Each volume may include a probe having a label, a sink having a quencher and configured to hybridize with the probe to quench the label, and a separator configured to hybridize with the probe and/or the sink to block hybridization of the sink with the probe. An amplification reaction may be performed in the volumes to generate an amplicon corresponding to the target. The separator may hybridize with the amplicon, and may be extended or degraded by the amplification reaction. Photoluminescence of the label may be detected from the volumes, and the photoluminescence of target-positive volumes may be less than that of target-negative volumes.
Abstract: Methods and reagents for blocking non-specific interactions with nucleic acids are disclosed. In particular, the invention relates to multi-valent blockers comprising multiple negatively charged polymers or materials attached to a common scaffold and their use in blocking non-specific interactions with nucleic acids.
Abstract: The disclosure is directed to methods, kits, and compositions, for amplifying and detecting a human hepatitis B virus (HBV) in a sample, which comprises a variety of combinations of forward oligonucleotide primers, reverse oligonucleotide primers, and oligonucleotide probes.
Type:
Grant
Filed:
September 27, 2018
Date of Patent:
June 8, 2021
Assignee:
ABBOTT MOLECULAR INC.
Inventors:
Dominik Duelli, Wai-Bing Mak, Brian Erickson, Amanda Goldston
Abstract: A method for carrying out nucleic acid amplification, includes providing a reaction chamber, accommodating an array of nucleic acid probes at respective locations, for hybridizing to respective target nucleic acids; and introducing a solution into the reaction chamber, wherein the solution contains primers, capable of binding to target nucleic acids, nucleotides, nucleic acid extending enzymes and a sample including nucleic acids. The a structure of the nucleic acid probes and of the primers so that a hybridization temperature of the probes is higher than an annealing temperature of the primers, whereby hybridization and annealing take place in respective separate temperature ranges.
Abstract: An apparatus suitable for single molecule sequencing. The apparatus includes at least one nanowell, a plurality of nucleic acid immobilization moieties, and a plurality of types of nucleic acid fragments. The nanowell has an observation zone. The nucleic acid immobilization moieties are disposed in or proximate to the observation zone. The nucleic acid fragments are immobilized to the nucleic acid immobilization moieties, respectively. At least one polymerase is disposed in the observation zone. A method of sequencing nucleic acid molecules using the above-mentioned apparatus is provided.
Abstract: A high-throughput and rapid nucleic acids detection method based on capillary microarrays comprises the steps that firstly, microarray containing a number of hydrophilic and vertical micro-channels is fabricated by capillary assembling, casting and machining, and the outer surface of the capillary array is coated with super-hydrophobic Ultra-Ever Dry paint; secondly, different primer sets are individually loaded into the micro-channels and air-dried to adhere them on the inner surface, and then the microarray is anchored into a reaction tube; thirdly, the reaction mixture is introduced into every microchannel at once through capillary force by a special designed sample-loading adaptor, and then the amplification reaction is performed in the temperature control device; and finally, the fluorescence can either be measured continually during the reaction for real-time detection or be recorded once in the end for endpoint detection. Moreover, the products can also be recovered for other use later.