Abstract: The present invention relates to a library of particles, the library displaying a plurality of different T cell receptors (TCRs), wherein the plurality of TCRs consists essentially of TCRs comprising an alpha chain variable domain and a beta chain variable domain, wherein the alpha chain variable domain comprises a TRAV12-2 gene product and the beta chain variable domain comprises a TRBV gene product.
Type:
Grant
Filed:
August 19, 2021
Date of Patent:
August 29, 2023
Assignees:
Immunocore Limited, Adaptimune Limited
Inventors:
Bent Karsten Jakobsen, Nathaniel Ross Liddy, Peter Eamon Molloy, Annelise Brigitte Vuidepot
Abstract: Disclosed herein include systems, methods, compositions, and kits for determining the expression of highly expressed proteins and lowly expressed proteins. In some embodiments, primers allowing generation of separate libraries for abundant AbSeq protein profiling oligonucleotides and scarce AbSeq protein profiling oligonucleotides are provided.
Type:
Grant
Filed:
November 19, 2021
Date of Patent:
August 29, 2023
Assignee:
Becton, Dickinson and Company
Inventors:
Cynthia Sakofsky, Katherine Lazaruk, Margaret Nakamoto, Devon Joseph Jensen
Abstract: The present disclosure discloses an antibody fragment library, method for preparing the library and its applications. The essential steps in construction of the library is devoid of any restriction enzyme. Emulsion based PCR has been used as an important tool for the construction and validation of the library. The method as disclosed in the present disclosure leads to construction of a library comprising at least 8 billion clones.
Abstract: The present invention relates generally to the field of making novel antigen binding domains against infectious diseases. The present invention also relates to novel CARs that utilize the novel antigen binding domains as an extracellular element. The present invention also relates to use of the novel antigen binding domains as therapeutic agents.
Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.
Type:
Grant
Filed:
May 28, 2020
Date of Patent:
July 18, 2023
Assignee:
GigaGen, Inc.
Inventors:
David Scott Johnson, Adam Adler, Rena Mizrahi
Abstract: A method for creating a plasmid for use in producing a chimeric antibody, comprising (a) receiving a FAB region of the antibody; (b) receiving a fluorescent protein; (c) receiving a linker having length of at least 5 amino acids; (d) using the Gibson assembly process to join the FAB region, the fluorescent protein, and the linker into an expression plasmid.
Abstract: A visual continuous spatial directed evolution method is disclosed. The host grows and moves in a solid culture space, the host carrying a foreign target gene to be evolved and containing a gene element that assists the evolution of the target gene, the target gene being correlated with the growth and movement of the host. Depending on different spatial distribution patterns formed in the solid culture space during the growth and movement of the host, screening is performed to obtain an evolved product. This method is carried out directly in the solid culture space. Depending on images of different spatial distribution morphologies visible to the naked eye that are locally formed, selection of evolved products is performed without the need for liquid fed-batch culture equipment. In addition, the evolution effect is visually observed through the infection spots formed during evolution, so that no real-time monitoring equipment is required.
Type:
Grant
Filed:
June 17, 2020
Date of Patent:
July 18, 2023
Assignee:
Shenzhen Institutes of Advanced Technology
Abstract: [Problem] Provided are a production method for a multi-input/multi-output-type genetic switch or a transcription factor, and a multi-input/multi-output-type genetic switch or a transcription factor. [Solving Means] The inventors of the present invention have completed a production method for a multi-input/multi-output-type genetic switch or a transcription factor, essentially including the steps of “fusing two or more transcription factor genes to each other” and “introducing mutations into the fusion-type transcription factor gene,” and have further succeeded in obtaining a multi-input/multi-output-type genetic switch or a transcription factor by the method.
Type:
Grant
Filed:
March 23, 2019
Date of Patent:
July 18, 2023
Assignee:
NATIONAL UNIVERSITY CORPORATION CHIBA UNIVERSITY
Abstract: The present invention provides a vector library for yeast two-hybrid screening of a deubiquitinating enzyme that binds to a target protein and a method for identifying a deubiquitinating enzyme binding to a target protein using the same. Also, the present invention provides a method for screening an agent having anti-cancer activity targeting the deubiquitinating enzyme USP1, USP7, USP12, or USP49 identified by said identifying method.
Type:
Grant
Filed:
August 6, 2018
Date of Patent:
June 27, 2023
Assignee:
CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
Inventors:
Kwang-Hyun Baek, So-Ra Kim, Seul-Ki Kwon, Soo-Yeon Kim, Da-Hye Lee
Abstract: The invention provides singleplex and multiplex assays for screening of antigen-binding molecules for their affinity to antigens by normalizing for the concentration of the antigen-binding molecule.
Abstract: The present disclosure application relates to a construction method of a yeast display library (YSD), specifically to a construct for a yeast display library, an expression vector, a host cell and a construction method and use thereof. The yeast display library provided by the present application has high transformation efficiency and rich diversity.
Type:
Grant
Filed:
May 17, 2021
Date of Patent:
June 20, 2023
Assignee:
ADLAI NORTYE BIOPHARMA CO., LTD.
Inventors:
Nanhai He, Dandan Chen, Yang Lu, Donghui Yang
Abstract: Universal antibody libraries are described which are synthetic and derived from expressed human antibody sequences selected accordingly to certain criteria, for example, that the sequences are derived from naturally-occurring antibodies expressed in response to a certain antigen class (e.g., small molecule, polysaccharide, peptide, or protein) and having CDR regions engineered for optimal diversity. Methods for making and screening such libraries for isolating therapeutics suitable for treating disease are also disclosed.
Abstract: The invention relates to a method for selecting a sequence set from a library of expressed nucleic acid sequences, wherein cells are provided, each cell comprises an expressed nucleic acid sequence expressed as a target protein. The cells are encapsulated by treating them with a cationic polysaccharide and subsequently treating them with an anionic polysaccharide, yielding encapsulated cells, perforating the membrane of the encapsulated cells, yielding solubilized compartments, contacting them with a ligand to said target protein, the ligand bearing a detectable label, and selecting a subset of solubilized compartments as a function of detectable label and isolating the expressed nucleic acid sequences from the selection as a selected sequence set.
Abstract: There is provided herein a method for identifying and/or recovering at least one genetically encoded affinity reagent specific for a target molecule by screening using molecular display in conjunction with the sequencing of positive and negative selection pools from the screen.
Type:
Grant
Filed:
August 14, 2020
Date of Patent:
May 16, 2023
Assignee:
THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
Inventors:
Sachdev Sidhu, Jason Moffat, Helena Persson, Nish Patel, Saravanan Sundararajan, Amandeep Gakhal, Wei Ye, Jelena Tomic, Megan Mclaughlin
Abstract: The present invention addresses the problem of providing a novel method which is for preparing a DNA fragment for microbial cell transformation, and by which the combinatorial library of a long-chain DNA can be efficiently constructed and confirmation of the genotype of the obtained clone is facilitated.
Type:
Grant
Filed:
March 24, 2020
Date of Patent:
May 9, 2023
Assignee:
NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
Abstract: The present disclosure provides a method for screening cells, the method including a step of preparing a plurality of cells which are tagged with a first barcode nucleic acid associated with a test target and treated with the test target, a step of sorting the plurality of cells based on cellular phenotype using an imaging cell sorter, and a step of identifying the test target used to treat each cell using the first barcode nucleic acid as an indicator.
Type:
Grant
Filed:
January 12, 2021
Date of Patent:
May 9, 2023
Assignees:
ThinkCyte, Inc., The University of Tokyo, RIKEN
Abstract: The present invention is relevant to proteins and novel methods of protein evolution. The present invention further relates to methods of identifying and mapping mutant polypeptides formed from, or based upon, a template polypeptide.
Abstract: Provided is a method for screening an in vitro display library for binding within a cell of a small-molecule chemical compound binding entity of the library to a protein or RNA target of interest in order to identify at least one individual chemical compound binding entity of the library that is capable of binding within the cell to the protein or RNA target of interest.
Type:
Grant
Filed:
January 16, 2020
Date of Patent:
April 25, 2023
Assignee:
VIPERGEN APS
Inventors:
Nils Jakob Vest Hansen, Jacob Andersen, Ole Kristensen, Allan Beck Christensen, Lars Kolster Petersen
Abstract: The purpose of the present invention is to provide a method for identifying antibody CDR3 clusters using high speed in vitro screening a library selected from the group consisting of cDNA library and nucleic acid aptamer library comprising: (i) preparing a positive spherical shaped structure by binding a target molecule to a spherical shaped molecule, wherein the target molecule is immobilized on the positive spherical shaped structure, wherein the positive spherical shaped structure may contain a fluorescent label; (ii) preparing a negative spherical shaped structure, wherein the target molecule is not immobilized on the negative spherical shaped structure, wherein the negative spherical shaped structure may contain a fluorescent label; (iii) forming a positive spherical shaped conjugate or a negative spherical shaped conjugate by binding a target detecting molecule capable of binding to the target molecule to the positive spherical shaped structure or to the negative spherical structure, wherein the target
Type:
Grant
Filed:
September 18, 2020
Date of Patent:
April 25, 2023
Assignee:
EPSILON MOLECULAR ENGINEERING INC.
Inventors:
Naoto Nemoto, Toshiki Miyajima, Yuta Matsukawa