Patents Examined by Christian Fronda
  • Engineered cleavage half-domains
    Patent number: 9376689
    Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
    Type: Grant
    Filed: February 20, 2015
    Date of Patent: June 28, 2016
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Yannick Doyon, Jeffrey C. Miller
  • Useful halophilic, thermostable and ionic liquids tolerant cellulases
    Patent number: 9376728
    Abstract: The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.
    Type: Grant
    Filed: June 11, 2012
    Date of Patent: June 28, 2016
    Assignees: The Regents of the University of California, Sandia Corporation
    Inventors: Tao Zhang, Supratim Datta, Blake A. Simmons, Edward M. Rubin
  • Flocculation method
    Patent number: 9371554
    Abstract: The present invention relates to a method for harvesting recombinant proteins from mammalian cell culture fluid. The method makes use of cationic polymers, non-ionic polymers and non-ionic surfactants.
    Type: Grant
    Filed: December 14, 2012
    Date of Patent: June 21, 2016
    Assignee: AMGEN INC.
    Inventors: Thomas M McNerney, Krista Petty, Anne C Thomas, Xiaoyang Zhao
  • Fermentive production of four carbon alcohols
    Patent number: 9365872
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: May 19, 2015
    Date of Patent: June 14, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
    Patent number: 9365843
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: May 8, 2015
    Date of Patent: June 14, 2016
    Assignee: Novozymes, Inc.
    Inventors: Yu Zhang, Junxin Duan, Lan Tang, Wenping Wu
  • Biotransformation using genetically modified
    Patent number: 9359581
    Abstract: A substantially pure Candida host cell is provided for the biotransformation of a substrate to a product wherein the host cell is characterized by a first genetic modification class that comprises one or more genetic modifications that collectively or individually disrupt at least one alcohol dehydrogenase gene in the substantially pure Candida host cell.
    Type: Grant
    Filed: November 19, 2013
    Date of Patent: June 7, 2016
    Assignee: Synthezyme LLC
    Inventors: Jon E. Ness, Jeremy Minshull
  • Processes of producing fermentation products
    Patent number: 9359621
    Abstract: The invention relates to a process of fermenting plant material in a fermentation medium into a fermentation product using a fermenting organism, wherein one or more carbonic anhydrases are present in the fermentation medium.
    Type: Grant
    Filed: April 7, 2014
    Date of Patent: June 7, 2016
    Assignee: NOVAZYMES NORTH AMERICA, INC.
    Inventors: Chee-Leong Soong, Jiyin Liu, Guillermo Coward-Kelly
  • Compositions and methods of PGL for the increased production of isoprene
    Patent number: 9353375
    Abstract: Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: May 31, 2016
    Assignees: DANISCO US INC., THE GOODYEAR TIRE & RUBBER COMPANY
    Inventors: Zachary Q. Beck, Marguerite A. Cervin, Alex T. Nielsen, Caroline M. Peres
  • Sclareol and labdenediol diphosphate synthase polypeptides, encoding nucleic acid molecules and uses thereof
    Patent number: 9353385
    Abstract: Provided are labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides, nucleic acid molecules encoding the labdenediol diphosphate synthase polypeptides and sclareol synthase polypeptides, and methods of using the labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides. Also provided are methods for producing labdenediol diphosphate, sclareol and (?)-ambroxide.
    Type: Grant
    Filed: July 30, 2013
    Date of Patent: May 31, 2016
    Assignee: Evolva, Inc.
    Inventors: Grace Eunyoung Park, Bryan N. Julien, Richard Burlingame
  • Bacterial iodoperoxidases from , methods of preparation and uses thereof
    Patent number: 9353354
    Abstract: The present invention concerns iodoperoxidases from Zobellia galactanivorans, isolated nucleic acids encoding same, as well as methods for preparing these enzymes. Moreover, the invention is also directed to the use of such iodoperoxidases in a wide range of industrial, pharmaceutical, medical, cosmetics, and ecological applications, as well as in the food industry.
    Type: Grant
    Filed: July 5, 2012
    Date of Patent: May 31, 2016
    Assignee: Centre National de la Recherche Scientifique
    Inventors: Catherine Leblanc, Ludovic Delage, Gurvan Michel, Etienne Rebuffet, Mirjam Czjzek, Philippe Potin
  • Method for the fermentative production of L-cysteine and derivatives of said amino acid
    Patent number: 9347078
    Abstract: The invention relates to a method for producing L-cysteine and its derivatives L-cystine and thiazolidine by fermenting a cysteine-producing microorganism strain in a fermentation medium. The invention is characterized in that L-methionine, L-isoleucine, or L-threonine in respective concentrations ranging from 0.1 to 10 g/L are added to the fermentation medium in the main culture.
    Type: Grant
    Filed: September 9, 2013
    Date of Patent: May 24, 2016
    Assignee: Wacker Chemie AG
    Inventors: Tobias Dassler, Walfred Leinfelder, Guenter Wich
  • Over-expression of a putative oxidoreductase () for increasing furfural or 5-hydroxymethylfurfural tolerance
    Patent number: 9347077
    Abstract: The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.
    Type: Grant
    Filed: July 9, 2012
    Date of Patent: May 24, 2016
    Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED
    Inventors: Xuan Wang, Elliot N. Miller, Lorraine P. Yomano, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
  • FAD-conjugated glucose dehydrogenase gene
    Patent number: 9340816
    Abstract: Disclosed herein are a gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase which can be characterized by reactivity to glucose, thermal stability, substrate-recognition performance, and low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; a method for the determination of glucose; a reagent composition for use in the determination of glucose; and a biosensor for use in the determination of glucose. An embodiment is a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide whose amino acid sequence comprises X1-X2-X3-X4-X5-X6, wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue.
    Type: Grant
    Filed: October 8, 2014
    Date of Patent: May 17, 2016
    Assignee: Ikeda Food Research Co., Ltd.
    Inventors: Takako Yada, Koji Miyamoto, Michinari Honda
  • Method for producing phosphoserine incorporated proteins by using SepRS mutants and EF-Tu mutants
    Patent number: 9322044
    Abstract: The present invention relates to a method of producing a phosphorylated protein using a SepRS (O-phosphoseryl-tRNA synthetase) mutant and an EF-Tu mutant, which have increased activity. More specifically, the invention relates to a method of producing a phosphorylated protein by incorporating phosphoserine into the specific position of a target protein or polypeptide using tRNASep serving to recognize at least one codon in the mRNA of the target protein or polypeptide, an O-phosphoseryl-tRNA synthetase (SepRS) mutant selected by a molecular evolution technique and serving to aminoacylate tRNASep with phosphoserine (Sep), and an EF-Tu mutant serving to bind and deliver Sep-tRNASep to the ribosome. According to the invention, a phosphorylated protein can be produced in an amount of mg per liter using the SepRS and EF-Tu mutants.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: April 26, 2016
    Assignee: Korea Advanced Institute of Science and Technology
    Inventors: Hee Sung Park, Sangsik Lee
  • Cloning, expression and use of acid phospholipases
    Patent number: 9322003
    Abstract: The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for by the plasmid pPL3940-Topo2.5 with the restriction map according to FIG.
    Type: Grant
    Filed: April 21, 2015
    Date of Patent: April 26, 2016
    Assignee: AB ENZYMES GMBH
    Inventors: Khanh Quoc Nguyen, Kornelia Titze, Tatiana Schwarz, Silvia Paladino, Volker Marschner, Patrick Lorenz
  • Thermostable cellulases, and mutants thereof, capable of hydrolyzing cellulose in ionic liquid
    Patent number: 9322042
    Abstract: The present invention provides for a composition comprising an ionic liquid and a thermostable cellulose, and a method of hydrolyzing a cellulose, comprising: (a) providing a composition comprising a solution comprising an ionic liquid and a cellulose, and (b) introducing a thermostable cellulase to the solution, such that the cellulose is hydrolyzed by the cellulase. The present invention also provides for a Thermatoga maritima thermostable cellulase mutant with increased cellulase activity.
    Type: Grant
    Filed: April 23, 2010
    Date of Patent: April 26, 2016
    Assignees: The Regents of the University of California, Sandia Corporation
    Inventors: Rajat Sapra, Supratim Datta, Zhiwei Chen, Bradley M. Holmes, Blake A. Simmons, Harvey W. Blanch
  • Method for the production of isobutanol by recombinant yeast
    Patent number: 9303225
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: July 23, 2014
    Date of Patent: April 5, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Michael Charles Grady, Mehmedalija Jahic, Lori Ann Maggio-Hall, Vasantha Nagarajan, Ranjan Patnaik
  • Metabolic engineering of arabinose-fermenting yeast cells
    Patent number: 9303253
    Abstract: The invention relates to an eukaryotic cell expressing nucleotide sequences encoding the ara A, ara B and ara D enzymes whereby the expression of these nucleotide sequences confers on the cell the ability to use L-arabinose and/or convert L-arabinose into L-ribulose, and/or xylulose 5-phosphate and/or into a desired fermentation product such as ethanol. Optionally, the eukaryotic cell is also able to convert xylose into ethanol.
    Type: Grant
    Filed: January 6, 2012
    Date of Patent: April 5, 2016
    Assignee: DSM IP ASSETS B.V.
    Inventors: Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Hendrik Wouter Wisselink, Johannes Pieter Van Dijken, Aaron Adriaan Winkler, Johannes Hendrik Winde
  • Fermentive production of four carbon alcohols
    Patent number: 9297029
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: December 30, 2014
    Date of Patent: March 29, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Modified Cry1Ca insecticial Cry proteins
    Patent number: 9284573
    Abstract: The present invention includes modified, insecticidal B.t. Cry1Ca proteins, including the proteins designated herein as DIG-109 and DIG-152, as well as variants of DIG-109 and DIG-152, nucleic acids encoding these proteins, methods of controlling pests using the proteins, methods of producing the proteins in transgenic host cells, and transgenic plants that produce the proteins. The DIG-109 and DIG-152 proteins comprise chimeric peptides composed of a core toxin segment of B.t. Cry1Ca and a Cry1Ab protoxin segment. Insecticidally active variants of the DIG-109 and DIG-152 proteins are also described.
    Type: Grant
    Filed: December 16, 2010
    Date of Patent: March 15, 2016
    Assignee: Dow AgroSciences LLC
    Inventors: Thomas Meade, Stephanie L. Burton, Kenneth Narva, Aaron T. Woosley, Timothy D. Hey, Ignacio M. Larrinua