Abstract: A single use lancet assembly having a housing, with an open interior and a piercing aperture defined therein, a lancet, with a body and a piercing tip positioned in the open interior of the housing and movable between a cocked orientation and a piercing orientation, a driving assembly structured to move the lancet into the piercing orientation, and a single use trigger assembly structured to actuate an actuating mechanism thereby allowing the lancet to move, at least temporarily, from its cocked orientation to its piercing orientation. The single use trigger assembly is structured such that it may not be positioned to actuate the actuating mechanism more than one time, thereby preventing inadvertent or intentional re-use of a contaminated piercing tip.