Patents Examined by David Thomas
-
Patent number: 10030023Abstract: The invention provides a mercaptoalkylglycoluril represented by the general formula (I): wherein R1 and R2 each independently represent a hydrogen atom, a lower alkyl group, or a phenyl group; R3, R4, and R5 each independently represent a hydrogen atom or a mercaptoalkyl group selected from a mercaptomethyl group, a 2-mercaptoethyl group, and a 3-mercaptopropyl group; and n is 0, 1, or 2. The invention further provides an epoxy resin composition comprising an epoxy resin and the mercaptoalkylglycoluril, and a method for producing a laminate or a multilayer printed circuit board using the same.Type: GrantFiled: November 28, 2014Date of Patent: July 24, 2018Assignee: SHIKOKU CHEMICALS CORPORATIONInventors: Takeshi Kumano, Takuma Takeda, Noboru Mizobe
-
Patent number: 9885075Abstract: The present invention concerns a method of detecting macroions in a liquid medium contained in a space, the method including: a) submitting the liquid medium to a stimulating electrical field to induce formation of aggregates of macroions, the formed aggregates of macroions preferably not including any additional labeling agent, and b) measuring, in a detection zone of the space, spatial and/or temporal fluctuations within the liquid medium of at least one variable depending on the concentration of the macroions in the liquid medium, and c) determining, based on these fluctuations, the presence of the macroions, step c) preferably including processing by a time-dependent or space dependent analysis, more preferably by wavelet analysis, or by autocorrelation the fluctuations measured at step b).Type: GrantFiled: July 2, 2013Date of Patent: February 6, 2018Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, INSTITUT CURIE, UNIVERSITE PIERRE ET MARIE CURIEInventors: Jean-Louis Viovy, François Amblard, Laurent Malaquin, Bastien Venzac, Mohamed Lemine Diakite, Stephanie Descroix, Ismaïl Cisse, Ulrich Bockelmann
-
Patent number: 9869682Abstract: The invention relates to a method for detecting the presence of a gynaecological growth, in particular for the diagnosis of endometriosis. The invention also relates to a method of identifying a biomarker for detecting the presence of a gynaecological growth and to biomarkers identified by said method.Type: GrantFiled: September 1, 2015Date of Patent: January 16, 2018Assignee: BELGIAN VOLITION SPRLInventors: Jacob Vincent Micallef, Mark Edward Eccleston
-
Patent number: 9862983Abstract: This invention covers methods for isothermal amplification of DNA, based on the unexpected discovery that primers having, at some positions, adenine substituted by 2-aminopurine or diaminopurine, guanine substituted by inosine, thymine substituted by 2-thiothymine, and cytosine substituted by N4-ethylcytosine are accepted by enzymes used in standard recombinase polymerase assays (RPA). Further unexpected was the discovery that target nucleotides are efficiently amplified in an RPA-like process (hereinafter abbreviated as simply RPA) using substituted primers. The invention also covers RPA-like processes that use substituted primers tagged with oligonucleotides incorporating nucleotides from artificially expanded genetic information systems (AEGIS).Type: GrantFiled: June 22, 2015Date of Patent: January 9, 2018Inventors: Steven Benner, Nilesh Karalkar
-
Patent number: 9863010Abstract: The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Adenovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers.Type: GrantFiled: February 1, 2016Date of Patent: January 9, 2018Inventors: Emily Ziegler, Jessica Townsend
-
Patent number: 9850526Abstract: The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer.Type: GrantFiled: May 26, 2015Date of Patent: December 26, 2017Assignee: President and Fellows of Harvard CollegeInventors: Jeremy Agresti, Liang-Yin Chu, David A. Weitz, Jin-Woong Kim, Amy Rowat, Morten Sommer, Gautam Dantas, George Church
-
Patent number: 9834815Abstract: The present teachings generally relate to methods and kits incorporating a discriminating positive control for determining whether a particular microorganism or group of microorganisms are present in a sample, for example but not limited to a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample. According to certain methods, at least part of a starting material, for example but not limited to, a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample can be combined with a culture medium and incubated under conditions suitable for microbial growth followed by extracting microorganism and added control nucleic acids for analysis.Type: GrantFiled: March 25, 2010Date of Patent: December 5, 2017Assignee: Life Technologies CorporationInventors: Michael Brewer, Olga Petrauskene, Jen-Kuei Liu, Craig Cummings, Sueh-Ning Liew
-
Patent number: 9822405Abstract: The present invention provides methods, reagents and kits for carrying out a variety of assays suitable for analyzing polynucleotides or samples that include an amplification step performed in a multiplex fashion. Also provided are methods for analyzing and improving the efficiency of amplification and for carrying out gene expression analysis.Type: GrantFiled: June 26, 2015Date of Patent: November 21, 2017Assignee: APPLIED BIOSYSTEMS, LLCInventors: Mark Andersen, David Ruff
-
Patent number: 9822401Abstract: The disclosure provides methods and systems for nucleic acid amplification including isothermal nucleic acid amplification.Type: GrantFiled: April 16, 2015Date of Patent: November 21, 2017Assignee: GENAPSYS, INC.Inventor: Florian Oberstrass
-
Patent number: 9822403Abstract: A non-contact system for sorting monodisperse water-in-oil emulsion droplets in a microfluidic device based on the droplet's contents and their interaction with an applied electromagnetic field or by identification and sorting.Type: GrantFiled: May 19, 2014Date of Patent: November 21, 2017Assignees: Lawrence Livermore National Security, LLC, The Regents of the University of CaliforniaInventors: Neil Reginald Beer, Abraham Lee, Andrew Hatch
-
Patent number: 9822396Abstract: Methods compositions and kits are provided for performing a chromatin or chromosome conformation capture assay in partitions.Type: GrantFiled: February 13, 2015Date of Patent: November 21, 2017Inventors: Claudia Litterst, Svilen Tzonev, Jeremy Agresti
-
Patent number: 9816121Abstract: The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer.Type: GrantFiled: March 3, 2017Date of Patent: November 14, 2017Assignee: President and Fellows of Harvard CollegeInventors: Jeremy Agresti, Liang-Yin Chu, David A. Weitz, Jin-Woong Kim, Amy Rowat, Morten Sommer, Gautam Dantas, George Church
-
Patent number: 9809841Abstract: The present invention provides a method of detecting nucleotide sequence differences between two nucleic acid samples. The method employs a comparative genomic hybridization (CGH) technique to analyze the sequence differences between the samples. This method permits the identification of small sequence differences (e.g., sequence divergence of 1% or less) in nucleic acid samples of high complexity (e.g., an entire genome).Type: GrantFiled: April 20, 2016Date of Patent: November 7, 2017Assignee: The Regents of the University of CaliforniaInventors: Donna G. Albertson, Daniel Pinkel, Yevgeniya Fridlyand, Bing Huey, Antoine Snijders
-
Patent number: 9809846Abstract: The invention provides compositions comprising rolling circle amplification sequences and hairpin sequences specifically designed for the accurate and highly sensitive detection of one or more analyte sequences. The invention further provides kits comprising them and methods for their use.Type: GrantFiled: December 30, 2014Date of Patent: November 7, 2017Assignee: Yissum Research Development Company of The Hebrew University Of Jerusalem Ltd.Inventors: Itamar Willner, Fuan Wang, Chun-Hua Lu, Xiaoqing Liu, Lina Freage
-
Patent number: 9803188Abstract: The present disclosure relates to systems and methods for nucleic acid isolation. In particular, the present disclosure provides systems and methods for isolating nucleic acids from aqueous samples (e.g., blood or urine).Type: GrantFiled: December 21, 2012Date of Patent: October 31, 2017Assignee: IBIS BIOSCIENCES, INC.Inventors: Mark W. Eshoo, Christopher Crowder
-
Patent number: 9797008Abstract: In relation to an automated nucleic acid processor and an automated nucleic acid processing method using a multi function dispensing unit, processing involving extraction and amplification of the nucleic acid, can be consistently, quickly and efficiently conducted at a low cost with the use of a multi function dispensing unit, while saving user's trouble without expanding the scale of the device.Type: GrantFiled: May 8, 2015Date of Patent: October 24, 2017Assignee: UNIVERSAL BIO RESEARCH CO., LTD.Inventor: Hideji Tajima
-
Patent number: 9791409Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.Type: GrantFiled: May 11, 2015Date of Patent: October 17, 2017Assignee: Intelligent BioSystems, Inc.Inventors: Steven Gordon, Thomas Hagerott, Edmund Golaski, Jerzy Olejnik
-
Patent number: 9790540Abstract: The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5?-activated, 3?-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides.Type: GrantFiled: September 13, 2013Date of Patent: October 17, 2017Assignee: EPICENTRE TECHNOLOGIES CORPORATIONInventors: Ramesh Vaidyanathan, Scott Kuersten, Ken Doyle
-
Patent number: 9777315Abstract: The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a component comprising 3? exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3? exonuclease activity; (d) extending the 3? end of at least one nucleic acid domain of said duplex to generate an extension product, whereinType: GrantFiled: January 30, 2012Date of Patent: October 3, 2017Assignee: OLINK PROTEOMICS ABInventors: Simon Fredriksson, Martin Lundberg, Anna Eriksson, Emma Rennel-Dickens
-
Patent number: 9771615Abstract: Disclosed is a method for sequencing a polynucleotide analyte comprising: •a. generating a stream of droplets containing a single nucleotide wherein the order of single nucleotides in the droplet stream corresponds to the sequence of nucleotides in the analyte; •b. introducing into each droplet a plurality of biological probe types each type comprising a different label in an undetectable state and being adapted to capture a different single nucleotide; •c. causing the single nucleotide contained in the droplet to bind to its complementary probe and •d. causing the label to be released from the probe that has bound the nucleotide in a detectable state. The probe is a dumbbell shaped probe comprising fluorescent donor and quencher labels and a single nucleotide gap. After gap repair by a polymerase and a ligase, a restriction enzyme recognition site is cleaved by a restriction enzyme, followed by exonuclease digestion to release the labels.Type: GrantFiled: October 4, 2013Date of Patent: September 26, 2017Assignee: BASE4 INNOVATION LTDInventors: Cameron Alexander Frayling, Barnaby Balmforth, Bruno Flavio Nogueira de Sousa Soares, Thomas Henry Isaac, Boris Breiner, Alessandra Natale, Michele Amasio