Abstract: A method of producing microbial biomass include providing a wastewater stream and determining the concentration of micronutrients selected from the group consisting of aluminum, boron, cobalt, magnesium, manganese, and zinc, and any combination thereof, in the wastewater stream. The method also includes determining the biological oxygen demand (BOD) normalized dose of the micronutrients, and modulating the concentration of at least one micronutrient in the wastewater stream to provide a micronutrient-modulated wastewater stream. The method further includes growing microbial biomass in the micronutrient-modulated wastewater stream.

Abstract: The invention provides a process for the production of a cementitious material, comprising mixing cement starting materials, a healing agent and a fibrous reinforcing material, wherein the healing agent comprises bacterial material, and wherein the fibrous reinforcing material comprises a biodegradable polymer, having an average molecular weight selected from the range of 10-1500 kg/mol, and wherein the fibrous material comprises fibers having diameters selected from the range of 5-750 ?m, and having lengths selected from the range of 50 ?m-150 mm.

Abstract: Methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood) are described. The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).

Abstract: The disclosure relates to enzyme producing Bacillus strains that provide benefits to animals and methods of using these strains. In one embodiment, the disclosure relates compositions comprising the enzyme producing Bacillus strains. In yet another embodiment, the disclosure relates to a feed for an animal comprising enzyme producing Bacillus strains.

Abstract: Provided are a composition containing as active ingredients thereof Bacillus subtilis strain BN1001 (deposit number: NITE BP-02608 and Bacillus subtilis var. natto, a wastewater treatment system provided with a biological treatment tank that biologically treats wastewater wherein the Bacillus subtilis strain BN1001 and Bacillus subtilis var. natto are contained in the biological treatment tank, a wastewater treating method that biologically treats wastewater comprising a step for adding the Bacillus subtilis strain BN1001 and Bacillus subtilis var. natto to the wastewater, a deodorization method comprising a step for contacting the Bacillus subtilis strain BN1001 and Bacillus subtilis var. natto with a malodorous source, a support having Bacillus subtilis and Bacillus subtilis var.

Abstract: A method and device for warming biological samples utilizes a heater and an outer chamber fabricated from a thermal regulating material. The outer chamber includes an outer surface and an inner surface with the heater being in operative contact with at least a portion of the outer surface of the outer chamber. A biological sample receiving region defined by the inner surface of the outer chamber is included, the biological receiving region being configured to operatively accept a biological sample and, alternatively including a liquid that has a freezing point near to or below that of the biological sample, and, wherein one or more of the inner surface and the liquid is in close and intimate contact with the biological sample. The thermal regulating material may obtain and maintain a substantially constant predetermined temperature when heat is imparted to the outer chamber by the heater.

Abstract: The present disclosure relates to a hydrogel derived from a methacrylated or acrylated natural polymer and a synthetic polymer, and a method of preparing the same. The disclosure further relates to 3D scaffolds and implants comprising said hydrogel.

Abstract: The invention relates to a process for producing a microbial storage compound, in particular polyhydroxyalkanoate, using micro-organisms capable of accumulating such microbial storage compound, wherein such micro-organisms are selected and the microbial storage compound is accumulated by carrying out the so-called feast phase of the selection step and the accumulation of the microbial storage compound in selected micro-organisms in the same reactor and carrying out the so-called famine phase of the selection step in a separate, smaller, reactor.

Abstract: A method of identifying and preferably quantifying at least one sphingolipid, in particular the sphingolipid portion in wild-type Cordyceps or Cordyceps derivates includes preparing a test sample solution from a Cordyceps sample, subjecting the test sample solution to liquid chromatrography with a mobile phase including at least a first and second eluting solvent; and performing a mass spectrometry after the step of subjecting. The quality and safety of Cordyceps products can be determined and wild-type Cordyceps and Cordyceps derivates may be differentiated via the ratio and/or presence of certain sphingolipids especially suitable as markers.

Abstract: A method of preparing an allogenic dendritic stem cell vaccine for treating ovarian cancers is disclosed. The method comprises, (a) collecting blood sample from an allogenic donor in a sterile collection bag, (b) separating viable mesenchymal stem cells inoculums by centrifuging the blood sample, suspending said mesenchymal stem cells inoculum in proliferation medium, plating the cells in a flask and incubated, and collecting cells adherent to the flask and further harvesting and expanding said adherent cells, (c) preparing myeloid dendritic cells by isolating, harvesting and culturing said blood sample source using myeloid dendritic isolation kit, (d) mixing the adherent cells obtained. Finally, (e) the composition of the adherent cells obtained in step (d) to the myeloid dendritic cells obtained in step (c) at a ratio of 90:10 and printing the cellular vectors with patient tumor derived heterogeneous progenitors.

Abstract: Nutritional supplement compositions that may provide sub-cellular phytonutrient delivery include a carrier oil base containing at least one triglyceride and also contain at least one biologically active complex in the carrier oil base. The biologically active complex may include a non-covalent complex of a carbon-60 fullerene, phytonutrient compound, and a triglyceride from the carrier oil base. The phytonutrient compound may be chosen from compounds having the ability to form non-covalent complexes with six-membered aromatic rings of carbon-60 fullerene. Examples of such phytonutrient compound include, for example, phenolic compounds, polyphenolic compounds, phenolic acids, flavonoids, terpenoids, tannins, stilbenes, curcuminoids, coumarins, lignans, quinones, phenylethanoids, carotenoids, astaxanthin, zeaxanthin, or coenzyme Q10. The nutritional supplement compositions may include multiple biologically active complexes in which the phytonutrient compounds are derived from olives, algae, or both.

Abstract: The disclosure relates to an activated platelet preservation composition, a method for preserving activated platelet and a preserved activated platelet using the same. Here, the disclosure relates to an activated platelet preservation composition comprising a divalent cation, chloride, vitamin B, a selenium source and a solvent, a kit using the same, a method for preserving an activated platelet, and a preserved activated platelet using the same. According to the disclosure, it is possible to obtain and preserve platelets, which are positive for CD61 and CD62p and negative for PAC-1 among cell markers.

Abstract: Growth medium are disclosed for use in fermenting a marine microorganism. The medium comprise Potassium, Calcium, Strontium, Borate and Fluoride at specific concentrations. Alternatively, the growth medium comprises cobalt at specified concentrations or comprises vitamin B12 at specified concentrations. Methods of producing certain desired compound by fermentation of a marine microorganism are also disclosed.

Abstract: The present invention provides methods and apparatus for enhanced detection of a disease by among others enhancing the difference in a microscopic property of diseased cells and normal cells, thereby enhancing the detection sensitivity and specificity.

Abstract: An apparatus and method of measuring biofilm and biological activity on a surface is disclosed. The apparatus includes a biofilm, which includes one or more microorganisms, grown on a substrate. A viewing window is placed on a surface of the biofilm and a gas bubble is introduced between the viewing window and the surface of the biofilm. The space between the substrate and the viewing window may be enclosed in a casing that has an inlet and an outlet, forming a flow cell. A microscope system, such as a white light interferometer, captures data of the biofilm in situ and non-destructively. The 3D images of biofilm surface have high resolution while maintaining a large field of view. The apparatuses and methods will be useful for fundamental studies of biofilms, biomedical and environmental screening, and many other applications in biology and the life sciences.

Abstract: Methods of treating a plant exposed to a phytotoxicant are provided. Embodiments of the subject methods include identifying a plant exposed to a phytotoxicant and applying an assimilable carbon-skeleton energy component-comprising composition to the identified plant. Embodiments of the subject compositions may include one or more of a macronutrient component, micronutrient component, vitamin/cofactor component, complexing agent and microbe. Kits for use in practicing the subject invention are also provided. The subject methods find use in a variety of different applications in which a plant is phytotoxic or at least in danger of becoming phytotoxic due to exposure or potential exposure to a phytotoxicant.

Abstract: The disclosure relates in one aspect to a fast acidifying lactic acid bacterium that generates a viscosity in fermented milk greater than about 62 Pa·s after 14 days of storage at 6° C. Further, the disclosure includes method for preparing and products of food, food additive, feed, nutritional supplement, or probiotic supplement, thereof.

Abstract: A process for preserving the initial fermentable potential of an organic substrate over time is presented. The method comprises a) determining the moisture content (HM) of the organic substrate, b) comparing the determined moisture content (HM) to a predefined moisture level (HD), c) storing only the organic substrate of which the moisture content (HM) determined in step a) corresponds to the predefined moisture content (HD), d) when a fermentable potential cannot be determined based on the moisture content (HM) alone during steps a) and c), taking measurements of the methanogenic potential of the biomass, e) comparing the measurements of the methanogenic potential of the biomass obtained during step c) to that of step a), and f) adapting the storage conditions to keep the moisture content (HM) of the organic substrate collected at a value that of the predefined moisture content (HD).

Abstract: A method and system removes biological contaminants from an algal crop. A flow of an algal crop containing biological contaminants is provided to a hydrodynamic separator (HDS) system. The flow is controlled by a control mechanism, wherein the biological contaminants are concentrated into a first portion of the flow of the algal crop within the HDS system, based on the size of the biological contaminants, and the first portion contains a majority of the biological contaminants in the flow of the algal crop. A second portion of the flow contains a majority of algal in the flow of the algal crop. The flow of the algal crop is split by use of a bi-furcated output of the HDS system. The splitting is between the first portion containing the majority of the biological contaminants in the flow of the algal crop, and the second portion which contains the majority of the algal of the algal crop.

Abstract: This disclosure provides compositions formulated in the form of tablets, wafer capsules, gel capsules, sticks, sachets, vials, droppers or in injectable form. The compositions comprise a mixture of: an extract of Chrysanthellum indicum, an extract of Cynara scolymus, an extract of Vaccinium myrtillus, an extract of Olea europaea, and pipeline. This disclosure also provides methods of treating pathological disorders of carbohydrate and/or fat metabolism in a patient by administering to the patient the compositions.