Patents Examined by Gary Benzion
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Patent number: 11840725Abstract: The present disclosure provides methods for detecting a single-stranded target RNA. The present disclosure provides methods of cleaving a precursor C2c2 guide RNA array into two or more C2c2 guide RNAs. The present disclosure provides a kit for detecting a target RNA in a sample.Type: GrantFiled: February 21, 2019Date of Patent: December 12, 2023Assignee: The Regents of the University of CaliforniaInventors: Jennifer A. Doudna, Mitchell Ray O′Connell, Alexandra East-Seletsky, Spencer Charles Knight, James Harrison Doudna Cate
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Patent number: 11789018Abstract: Methods for rapidly concentrating a food sample for efficient detection of bacteria are disclosed. A microfiltration approach followed by centrifugation was used to concentrate the cells with an enzyme (e.g., a protease) added at the beginning of the process to facilitate more efficient micro-filtering. The enzyme was found to have no significant effect on cell viability.Type: GrantFiled: July 22, 2016Date of Patent: October 17, 2023Assignee: Purdue Research FoundationInventors: Michael Ralph Ladisch, Eduardo de Aquino Ximenes, Seockmo Ku, Kirk Solon Foster, Thomas Richard Kreke, Xingya (Linda) Liu, James Thomas Jones, Amanda Deering, Jaycey Hardenstein, Alisha Tungare
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Patent number: 11634783Abstract: The present invention provides a method for detecting fungal DNA in a turf grass sample with a loop-mediated isothermal amplification (LAMP) assay which contains primers for fungal DNA of at least one turf pathogenic fungi selected from Sclerotinia homoeocarpa, Rhizoctonia solani spp., Pythium ashanidematum, Gaeumannomyces graminis spp., Microdochium nivale spp., Magnaporthe poae, Colletotrihum graminicola, Colletotrichum cereale and Pytium ultimum var. ultimum, comprising: subjecting the turf sample to a LAMP reaction wherein the LAMP reaction uses a primer set of four or more nucleic acid sequences with each primer in the set having from 15 to 50 nucleic acids The primers useful in the present method are selected from specifically selected internal transcribed spacer regions or genes of the target fungi to provide improved assay results.Type: GrantFiled: February 5, 2019Date of Patent: April 25, 2023Assignee: SYNGENTA PARTICIPATIONS AGInventors: Marilena Palmisano, Christian Wohler
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Patent number: 11612894Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.Type: GrantFiled: January 26, 2017Date of Patent: March 28, 2023Assignee: ANDE CORPORATIONInventors: Richard F. Selden, Eugene Tan
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Patent number: 11612893Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.Type: GrantFiled: December 2, 2016Date of Patent: March 28, 2023Assignee: ANDE CORPORATIONInventors: Richard F. Selden, Eugene Tan
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Patent number: 11602750Abstract: A sample processing tubule is provided including, from a proximate to a distal end, an opening through which a sample is introducible, at least three segments, and an reagent introduction port operatively connected to a distal segment of the at least three segments. The reagent introduction port enables the addition of a reagent in the distal segment of the tubule, enabling the user to create a customizable assay tubule.Type: GrantFiled: May 25, 2018Date of Patent: March 14, 2023Assignee: Roche Molecular Systems, Inc.Inventors: Thomas Drechsler, Nancy Schoenbrunner
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Patent number: 11542562Abstract: The invention relates to a single nucleotide polymorphism (SNP) marker related to a Chinese horse short stature trait. The SNP molecular marker is located at the 501th position of a sequence shown in SEQ ID NO.1, polymorphism is G/A, and the SNP marker corresponds to base pair 18,205,998 on chromosome 8 in a horse. The SNP marker related to the Chinese horse short stature trait and use thereof provided by the present invention have the following advantages that: (1) the molecular marker is not restricted by the age, sex and the like of Chinese horses, is used in early breeding of the Chinese horses, performs accurate screening immediately at birth, and significantly promotes the breeding process of dominant pony varieties of the Chinese horse; (2) a method for detecting SNP of a Chinese horse TBX3 gene is accurate, reliable, and easy to operate.Type: GrantFiled: September 2, 2020Date of Patent: January 3, 2023Assignee: INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Yuehui Ma, Lin Jiang, Xuexue Liu, Yabin Pu, Yanli Zhang
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Patent number: 11459598Abstract: This invention is directed to a method for detecting near and distant interactions between cells, organelles and other large structures. This method can also be used for specific detection and quantification of Antibody or other binding moiety protein-protein and macro-molecular interactions, as well as quantitative analysis of multiple proteins and their modifications.Type: GrantFiled: October 20, 2016Date of Patent: October 4, 2022Assignee: Quateris LLCInventors: Malcolm MacKenzie, Ilya Alexandrov, Michael Sherman
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Patent number: 11326202Abstract: The present invention provides methods, compositions and kits for enriching and determining nucleotide sequences of a plurality of target loci from a sample comprising nucleic acids. The methods comprise one or more cycles of primer extension followed by PCR amplification of target sequences using nested target-specific primers.Type: GrantFiled: November 23, 2018Date of Patent: May 10, 2022Assignee: HELITEC LIMITEDInventor: Zongli Zheng
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Patent number: 11286522Abstract: The present disclosure relates to systems and methods for high efficiency electronic sequencing of nucleic acids and molecular detection.Type: GrantFiled: August 20, 2018Date of Patent: March 29, 2022Assignee: GENAPSYS, INC.Inventor: Hesaam Esfandyarpour
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Patent number: 11155864Abstract: A method including steps of (a) providing an array of sites, wherein each site comprises a mixture of different nucleic acid templates; (b) extending primers hybridized to the different nucleic acid templates at each of the sites with different nucleotide analogs having different reversible blocking moieties, respectively, thereby producing different primer extension products at each site; (c) detecting the different primer extension products to distinguish the different nucleotide analogs at each site; and (d) removing the different reversible blocking moieties from the primer extension products at each of the sites using a first treatment that is selective for a first of the different reversible blocking moieties and a second treatment that is selective for a second of the different reversible blocking moieties.Type: GrantFiled: July 8, 2016Date of Patent: October 26, 2021Assignee: Illumina, Inc.Inventors: Eliane H Trepagnier, Tarun Khurana
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Patent number: 11001882Abstract: Provided are methods of producing a product nucleic acid. The methods include combining a template deoxyribonucleic acid (DNA), a polymerase, a template switch oligonucleotide, and dNTPs into a reaction mixture. The components are combined into the reaction mixture under conditions sufficient to produce a product nucleic acid that includes the template DNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.Type: GrantFiled: June 27, 2016Date of Patent: May 11, 2021Assignee: Takara Bio USA, Inc.Inventors: Craig Betts, Andrew Alan Farmer, Nathalie Bolduc
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Patent number: 10995361Abstract: The present invention relates to a method for amplifying at least one target RNA in a fixed and, optionally, expanded biological sample. In an embodiment of the invention, the method comprises incubating the fixed biological sample with a pair of polynucleotides complementary to non-overlapping and proximal sequences of a target RNA, wherein the polynucleotide pair hybridizes to the target RNA; ligating the polynucleotide pair using a ligase; and amplifying the ligation product. The invention further provides methods for detecting and optionally quantifying and/or sequencing the amplification product. As the method comprises hybridizing polynucleotide pairs to a target RNA in a fixed biological sample, the target RNA can be hybridized in situ.Type: GrantFiled: January 22, 2018Date of Patent: May 4, 2021Assignee: Massachusetts Institute of TechnologyInventors: Fei Chen, Asmamaw T. Wassie, Shahar Alon, Adam Henry Marblestone, Anubhav Sinha, Andrew Payne, Edward Stuart Boyden
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Patent number: 10801058Abstract: Disclosed is a method of quality control of nucleic acid amplification using quality control oligonucleotide. The method comprises a nucleic acid detection step and a determination step. The nucleic acid detection step comprises the steps of: preparing a nucleic acid sample containing a target nucleic acid and a quality control polynucleotide; preparing a compartment containing one molecule of the target nucleic acid and a compartment containing one molecule of the quality control polynucleotide; and carrying out nucleic acid amplification of the target nucleic acid and the quality control polynucleotide, in the compartments, and carrying out signal detection using a detection probe to detect a signal originated from the detection probe. In the determination step, it is determined as to whether or not the nucleic acid detection step is proper on the basis of the result obtained in the signal detection step.Type: GrantFiled: January 13, 2017Date of Patent: October 13, 2020Assignee: SYSMEX CORPORATIONInventors: Tsuyoshi Nakano, Yuichiro Yoshida
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Patent number: 10793922Abstract: The present invention provides methods for rapidly identifying an RNA viral infection using an isothermal nucleic acid amplification reaction that can be carried out extracted RNA in the context of a crude biological sample.Type: GrantFiled: October 20, 2015Date of Patent: October 6, 2020Assignee: ENVIROLOGIX INC.Inventors: Lars Peters, Stephen A. Judice, Daniel Shaffer, Breck Parker
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Patent number: 10787703Abstract: Genomic DNA contains embedded ribonucleotides (rNMPs) that are incorporated during DNA replication and repair, or formed during DNA damage. The modifications have been linked to genome instability and disease, but no method currently exists to profile their locations genome-wide. rNMP incorporation has been extensively studied in recent years; however, locating sites of rNMP incorporation in genomic DNA has not yet been possible. Disclosed herein is a unique method for mapping rNMPs in genomic DNA that exploits the unique ligation mechanism of Arabidopsis thaliana tRNA ligase (AtRNL), normally involved in tRNA maturation. As disclosed herein AtRNL captures 2?,3?-cyclic phosphate or 2?-phosphate termini of DNA derived from alkaline cleavage of a DNA oligonucleotide (oligo) at an embedded rNMP, ligating the 2?-phosphate end to the 5?-phosphate terminus of the same DNA molecule and producing a ssDNA circle containing an embedded rNMP.Type: GrantFiled: January 9, 2017Date of Patent: September 29, 2020Assignee: Georgia Tech Research CorporationInventors: Francesca Storici, Jay Hesselberth, Kyung Duk Koh
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Patent number: 10760074Abstract: A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.Type: GrantFiled: August 31, 2009Date of Patent: September 1, 2020Assignee: TAKARA BIO INC.Inventors: Kanako Usui, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
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Patent number: 10758908Abstract: A self-metering reaction device has a sample reservoir, configured to accept a varying amount of fluid; a metering reservoir, configured to be a subportion of the sample reservoir and to hold a reaction amount of the fluid; a reaction chamber fluidly connected to the metering reservoir; and a plunger comprising a tip configured to make a seal with the metering reservoir so that the reaction amount of the fluid is sealed within the metering reservoir when the plunger is in contact with the metering reservoir. The plunger can be configured to plunge the sealed reaction amount of the fluid from the metering reservoir into the reaction chamber.Type: GrantFiled: September 2, 2016Date of Patent: September 1, 2020Assignee: Tetracore, Inc.Inventors: William M. Nelson, Aymeric Randanne de Vazeille, Kyle Armantrout
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Patent number: 10745711Abstract: Compositions and methods related to transgenic high oleic acid/ALS inhibitor-tolerant soybean plants are provided. Specifically, the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean DP-305423-1 events are provided.Type: GrantFiled: October 18, 2017Date of Patent: August 18, 2020Assignee: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Anthony J Kinney, Kent Brink, Robert F. Cressman, Jr., Knut Meyer, Kevin L. Stecca, Natalie N. Weber, Cathy Xiaoyan Zhong
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Patent number: 10731227Abstract: Provided herein include compositions comprising a primer having the nucleic acid sequence of ACeIN-F3_c, a primer having the nucleic acid sequence of ACeIN-B3_a, a primer having the nucleic acid sequence of ACeIN-B3_b, a primer having the nucleic acid sequence of ACeIN-FIP_e, a primer having the nucleic acid sequence of ACeIN-FIP_f, a primer having the nucleic acid sequence of ACeIN-BIP (or ACeIN-BIP-song), a primer having the nucleic acid sequence of ACeIN-LF; and a primer having the nucleic acid sequence of ACeIN-LB. Also provided are methods of detecting human immunodeficiency virus (HIV) nucleic acids in a sample comprising performing reverse transcription-based loop mediated isothermal amplification (RT-LAMP) on a sample using the previously disclosed compositions.Type: GrantFiled: December 11, 2015Date of Patent: August 4, 2020Assignee: The Trustees of the University of PennsylvaniaInventors: Changchun Liu, Scott Sherrill-Mix, Haim H. Bau, Frederic D. Bushman, Karen E. Ocwieja, Jinzhao Song